Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-16T18:04:54.885Z Has data issue: false hasContentIssue false

Acid phosphatase activity demonstrated by intact Angiostrongylus cantonensis with special reference to its function

Published online by Cambridge University Press:  06 April 2009

Jun Maki
Affiliation:
Department of Parasitology, School of Medicine, Kitasato University, Sagamihara, Kanagawa Prefecture, Japan
Toshio Yanagisawa
Affiliation:
Department of Parasitology, School of Medicine, Kitasato University, Sagamihara, Kanagawa Prefecture, Japan

Summary

Intact Angiostrongylus cantonensis is able to hydrolyse glucose-phosphate esters, mononucleotides and p-nitrophenyl phosphate as well as β-glycerophosphate in vitro. Reciprocal inhibition studies suggest that the hydrolysis of such substrates is due to a non-specific phosphomonoesterase. Molybdate ions, which exert no effect on either the uptake of glucose or the production of lactate, inhibit the hydrolysis of glucose-1- phosphate in the external medium and simultaneously lower the production of lactate by the intact worms in vitro.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arme, C. & Read, C. P. (1970). A surface enzyme in Hymenolepis diminuta (Cestoda). Journal of Parasitology 56, 514–16.CrossRefGoogle ScholarPubMed
Barker, S. B. & Summerson, W. H. (1941). Colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry 138, 535–54.CrossRefGoogle Scholar
Bhaibulaya, M. (1968). A new species of Angiostrongylus in an Australian rat, Rattus fuscipes. Parasitology 58, 789–99.CrossRefGoogle Scholar
Bergmeyer, H. U. (1963). Methods of Enzymatic Analysis. New York: Academic Press.Google Scholar
Dike, S. C. & Read, C. P. (1971). Relation of tegumentary phosphohydrolase and sugar transport in Hymenolepis diminuta. Journal of Parasitology 57, 1251–5.CrossRefGoogle ScholarPubMed
Fiske, C. H. & Subbarow, Y. (1925). The colorimetric determination of phosphorus. Journal of Biological Chemistry 66, 375400.CrossRefGoogle Scholar
Inoue, I. (1959). Distribution of acid and alkaline phosphatases in the hairworm, Chordodes japonensis. Annotationes Zoologicae Japonenses 32, 209–13.Google Scholar
Levy, M. G. & Read, C. P. (1975). Relation of tegumentary phosphohydrolase to purine and pyrimidine transport in Schistosoma mansoni. Journal of Parasitology 61, 648–56.CrossRefGoogle ScholarPubMed
Lumsden, R. D. (1975). Parasitological review: surface ultrastructure and cytochemistry of parasitic helminths. Experimental Parasitology 37, 267339.CrossRefGoogle Scholar
Pappas, P. W. & Read, C. P. (1974). Relation of nucleotide transport and surface phosphohydrolase activity in Hymenolepis diminuta. Journal of Parasitology 60, 447–52.CrossRefGoogle ScholarPubMed
Phifer, K. (1960). Permeation and membrane transport in animal parasites: on the mechanism of glucose uptake by Hymenolepis diminuta. Journal of Parasitology 46, 145–53.CrossRefGoogle ScholarPubMed
Probert, A. J. (1969). Morphological and histochemical studies on the larval stages of Metastrongylus spp. (lungworms of swine) in the earthworm intermediate host, Eisenia foetida, Savigny 1826. Parasitology 59, 269–77.CrossRefGoogle Scholar
Starling, J. A. & Fisher, F. M. Jr (1975). Carbohydrate transport in Moniliformis dubius (Acanthocephala). 1. The kinetics and specificity of hexose absorption. Journal of Parasitology 61, 977–90.CrossRefGoogle Scholar
Yanagisawa, T. & Koyama, T. (1970). Glucose utilization, lactic acid production and glycogen content of ligated and non-ligated Dirofilaria immitis and distribution of acid phosphatase activity. The Joint Conference of Parasitic Diseases.The United States–Japan Cooperative Medical Science Program, 2223.Google Scholar
Yanagisawa, T., Koyama, T. & Futaba, M. (1970). Carbohydrate metabolism of Angiostrongylus cantonensis. (7) Glucose absorption by ligated worms and their phosphatase distribution. Japanese Journal of Parasitology 19, 992–3.Google Scholar
Yanagisawa, T., Koyama, T. & Ayai, S. (1973). Studies on glucose absorption through the tegument of adult Angiostrongylus cantonensis. Japanese Journal of Parasitology 22, 24.Google Scholar