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Alpha 2 giardin is an assemblage A-specific protein of human infective Giardia duodenalis

  • R. F. L. STEUART (a1), R. O'HANDLEY (a1), R. J. LIPSCOMBE (a2), R. A. LOCK (a2) and R. C. A. THOMPSON (a1)...

Of the 7 genetic assemblages of the parasite Giardia duodenalis only 2 (A and B) are known to cause infections in humans. These assemblages have been characterized in detail at the genomic level but few studies have examined differences in the proteins expressed. Employing one and two-dimensional PAGE we have identified an assemblage A-specific protein of human infective G. duodenalis; alpha 2 giardin. The protein difference was evident using both electrophoretic techniques. Alpha 2 giardin is known to be a structural protein and associates with the caudal flagella and the plasma membrane; however, its exact function is unknown. Although several proteins unique to assemblage B were also observed, we were unable to identify these proteins due to a lack of genomic data available for assemblage B isolates. Together, these proteins represent distinct phenotypic differences between the human infective assemblages of G. duodenalis and support the need to revise the taxonomy of this parasite.

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*Corresponding author: School of Veterinary and Biomedical Science, Murdoch University, Murdoch, 6150, Western Australia. Tel: +61 8 9360 2690. E-mail:
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G. Candiano , M. Bruschi , L. Musante , L. Santucci , G. M. Ghiggeri , B. Carnemolla , P. Orecchia , P. Zardi and P. G. Righetti (2004). Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 13271333.

A. G. Capon , J. A. Upcroft , P. F. L. Boreham , L. E. Cottis and P. G. Bundesen (1989). Similarities of Giardia antigens derived from human and animal sources. International Journal for Parasitology 19, 9198.

A. Gorg , C. Obermaier , G. Boguth , A. Harder , B. Scheibe , R. Wildgruber and W. Weiss (2000). The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21, 10371053.

A. Gorg , W. Weiss and M. J. Dunn (2004). Current two-dimensional electrophoresis technology for proteomics. Proteomics 4, 36653685.

S. P. Gygi , Y. Rochon , B. R. Franza and R. Aebersold (1999). Correlation between protein and mRNA abundance in yeast. Molecular and Cellular Biology 19, 17201730.

R. Haque , S. Roy , M. Kabir , S. E. Stroup , D. Mondal and E. R. Houpt (2005). Giardia assembalge A infection and diarrhoea in Bangladesh. Journal of Infectious Diseases 192, 21712173.

W. L. Homan and T. G. Mank (2001). Human giardiasis: genotype linked differences in clinical symptomatology. International Journal for Parasitology 31, 822826.

R. M. Hopkins , C. C. Constantine , D. A. Groth , J. D. Wetherall , J. A. Reynoldson and R. C. Thompson (1999). PCR-based DNA fingerprinting of Giardia duodenalis isolates using the intergenic rDNA spacer. Parasitology 118, 531539.

D. B. Keister (1983). Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 487488.

M. Lalle , A. Salzano , M. Crescenzi and E. Pozio (2006). The Giardia duodenalis 14-3-3 protein is post-translationally modified by phosphorylation and polglycylation of the C-terminal tail. Journal of Biological Chemistry 281, 51375148.

P. Monis , R. Andrews , G. Mayrhofer and P. Ey (1999). Molecular systematics of the parasitic protozoan Giardia intestinalis. Molecular Biology and Evolution 16, 11351144.

G. W. Moore , F. Sogandares-Bernal , M. V. Dennis , D. M. Root , D. Beckwith and D. Van Voorhis (1982). Characterization of Giardia lamblia trophozoite antigens using polyacrylamide gel electrophoresis, high-performance liquid chromatography, and enzyme-labelled immunosorbent assay. Veterinary Parasitology 10, 229237.

H. G. Morrison , A. G. McArthur , F. D. Gillin , S. B. Aley , R. D. Adam , G. J. Olsen , A. A. Best , W. Z. Cande , F. Chen , M. J. Cipriano , B. J. Davids , S. C. Dawson , H. G. Elmendorf , A. B. Hehl , M. E. Holder , S. M. Huse , U. U. Kim , E. Lasek-Nesselquist , G. Manning , A. Nigam , J. E. J. Nixon , D. Palm , N. E. Passamaneck , A. Prabhu , C. I. Reich , D. S. Reiner , J. Samuelson , S. Svard and M. L. Sogin (2007). Genomic minimialism in the early diverging intestinal parasite Giardia lamblia. Science 317, 19211926.

D. M. Moss , G. S. Visvesvara , H. M. Mathews and D. A. Ware (1992). Isoenzyme comparison of axenic Giardia lamblia strains. Journal of Protozoology 39, 559564.

T. E. Nash and D. B. Keister (1985). Differences in excretory-secretory products and surface antigens among 19 isolates of Giardia. Journal of Infectious Diseases 152, 11661171.

J. E. Palm , M. E. Weiland , W. J. Griffiths , I. Ljungstrom and S. G. Svard (2003). Identification of immunoreactive proteins during acute human giardiasis. Journal of Infections Disease 187, 18491859.

D. N. Perkins , D. J. C. Pappin , D. M. Creasy and J. S. Cottrell (1999). Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 35513567.

C. Read , J. Walters , I. D. Robertson and R. C. A. Thompson (2002). Correlation between genotype of Giardia duodenalis and diarrhoea. International Journal for Parasitology 32, 229231.

J. Sahaqun , A. Cavel , P. Goni , C. Seral , M. T. Llorente , F. J. Castillo , S. Capilla , A. Arias and R. Gomez-Lus (2008). Correlation between the presence of symptoms and the Giardia duodenalis genotype. European Journal of Clinical Microbiology and Infectious Diseases 27, 8183.

M. D. M. Siles-Lucas and B. Gottstein (2003). The 14-3-3 protein: a key molecule in parasites as in other organisms. Trends in Parasitology 19, 575581.

R. C. A. Thompson (2004). The zoonotic significance and molecular epidemiology of Giardia and giardiasis. Veterinary Parasitology 126, 1535.

M. E. Weiland , A. G. McCarthur , H. G. Morrison , M. L. Sogin and S. G. Svard (2005). Annexin-like alpha giardins: a new cytoskeletal gene family in Giardia lamblia. International Journal for Parasitology 35, 617626.

W. M. Wenman , R. U. Meuser and P. M. Wallis (1986). Antigenic analysis of Giardia duodenalis strains isolated from Alberta. Canadian Journal of Microbiology 32, 926929.

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