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An ultrastructural study on the role of Kupffer cells in the process of infection by Plasmodium berghei sporozoites in rats

Published online by Cambridge University Press:  06 April 2009

J. F. G. M. Meis ,
J. P. Verhave ,
P. H. K. Jap  and
J. H. E. Th. Meuwissen
J. F. G. M. Meis
Affiliation:
Department of Medical Parasitology, Geert Grooteplein Zuid 24, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
J. P. Verhave
Affiliation:
Department of Medical Parasitology, Geert Grooteplein Zuid 24, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
P. H. K. Jap
Affiliation:
Department of Cytology and Histology, Geert Grooteplein Noord 21, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
J. H. E. Th. Meuwissen
Affiliation:
Department of Medical Parasitology, Geert Grooteplein Zuid 24, University of Nijmegen, 6500 HB Nijmegen, The Netherlands
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Summary

The interactions in vivo between Plasmodium berghei sporozoites and Kupffer cells in rat livers were studied by transmission electron microscopy. By 10 and 15 min after inoculation, sporozoites were both free in the liver sinusoids and inside endocytotic vacuoles of the Kupffer cells. The latter cells were very active in phagocytosing sporozoites, bacteria and red blood cells. The sporozoites retained their integrity inside the endocytotic vacuoles and no signs of lysosomal digestion were observed. Sporozoites seen within endocytotic vacuoles 1 h after inoculation were still morphologically intact, although bristle-coated vesicles fused with the vacuole membrane. Evidence is presented which suggests that Kupffer cells transport sporozoites towards the space of Disse and adjacent hepatocytes. No sporozoites were seen to penetrate an endothelial cell or its narrow fenestrae. It is proposed that Kupffer cell passage, rather than gaps in the sinusoidal lining, represents the normal route that sporozoites take to circumvent the endothelial barrier. The localization of exo-erythrocytic forms was made easier by the use of Brown Norway rats in which many more parasites develop than in the Wistar rats. The distribution pattern of the parasites was found to be mainly around the ‘periportal’ zones of the acini of liver tissue.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 2 , April 1983 , pp. 231 - 242
Copyright
Copyright © Cambridge University Press 1983

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