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Asparaginyl endopeptidase activity in adult Schistosoma mansoni

  • J. P. Dalton (a1) (a2), L. Hola-Jamriska (a1) and P. J. Brindley (a1)


Sequence comparisons have recently shown that the Schistosoma mansoni protein Sm32 is similar to asparaginyl endo-proteinases, a novel family of cysteine proteinases, of which the legumains rom legumes are the best characterized. By synthesizing and employing fluorogenic peptide substrates for the specific detection of asparaginyl endopeptidases, we have identified this type of activity in extracts of adult S. mansoni. The S. mansoni activity is similar to that of the legumains in its substrate specificity and sensitivity to thiol inhibitors, but differs in its pH and temperature optima for activity. In contrast, unlike the legumains, the schistosome asparaginyl endopeptidase activity is not activated by the reducing agent dithiothreitol. As suggested for legumains, Sm32 may function in the post-translational modification processes that regulate the activity of other molecules.



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Abe, Y., Shirane, K., Yokasawa, H., Matsushita, H., Mitta, M., Kato, I. & Ishii, S.-I. (1993). Asparaginyl endopeptidase of jack bean seeds: purification, characterization, and high utility in protein sequence analysis. Journal of Biological Chemistry 268, 3525–9.
Bogitsh, B. J., Kirschner, K. F. & Rotmans, J. P. (1992). Schistosoma japonicum: immunoinhibitory studies on hemoglobin digestion using heterologous antiserum to bovine cathepsin D. Journal of Parasitology 78, 454–9.
Davis, A. H., Nanduri, J. & Watson, D. C. (1987). Cloning and gene expression of Schistosoma mansoni protease. Journal of Biological Chemistry 262, 12851–5.
Dalton, J. P., Smith, A. M., Clough, K. A. & Brindley, P. J. (1995). Digestion of haemoglobin by schistosomes: 35 years on. Parasitology Today (11, 273314)
Gotz, B. & Klinkert, M.-O. (1993). Expression and partial characterization of a cathepsin B-like enzyme (Sm31) and a proposed ‘haemoglobinase’ (Sm32) from Schistosoma mansoni. The Biochemical Journal 290, 801–6.
Kembhavi, A. A., Buttle, D. J., Knight, C. G. & Barrett, A. J. (1993). The two cysteine endopeptidases of legume seeds: purification and characterisation by use of specific fluorometric assays. Archives of Biochemistry and Biophysics 303, 208–13.
Klinkert, M.-O., Felleisen, R., Link, G., Ruppel, A. & Beck, E. (1989) Primary structure of Sm31/32 diagnostic proteins of Schistosoma mansoni and their identification as proteases. Molecular and Biochemical Parasitology 33, 113–22.
Klinkert, M.-Q., Ruppel, A. & Beck, E. (1987). Cloning of the diagnostic 31/32 kilodalton antigens of Schistosoma mansoni. Molecular and Biochemical Parasitology 25, 247–55.
Ruppel, A., Diesfeld, H. J. & Rothers, U. (1985). Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide. Clinical and Experimental Immunology 62, 499506.
Scott, M. P., Jung, R., Muntz, K. & Nielson, N. C. (1992). A protease responsible for post-translational cleavage of a conserved Asn-Gly linkage in glycinin, the major seed storage protein of soybean. Proceedings of the National Academy of Sciences, USA 89, 658662.
Smith, A. M., Dalton, J. P., Clough, K. A., Kilbane, C. L., Harrop, S. A., Hole, N. & Brindley, P. J. (1994). Adult Schistosoma mansoni express cathepsin L proteinase activity. Molecular and Biochemical Parasitology 67, 1119.
Takeda, O., Miura, Y., Mitta, M., Matsushita, H., Kato, I., Abe, Y., Yokosawa, H. & Ishi, S.-I. (1994). Isolation and analysis of cDNA encoding a precursor of Canavalia ensiformis asparaginyl endopeptidase (legumain). Journal of Biochemistry 116, 541–6.
Timms, A. R. & Bueding, E. (1959). Studies of a proteolytic enzyme from Schistosoma mansoni. British Journal of Pharmacology 14, 6873.


Asparaginyl endopeptidase activity in adult Schistosoma mansoni

  • J. P. Dalton (a1) (a2), L. Hola-Jamriska (a1) and P. J. Brindley (a1)


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