Skip to main content Accessibility help
×
Home

Characterization of Trichomonas vaginalis haemolysis

  • D. C. Dailey (a1), C. Te-Hung (a1) and J. F. Alderete (a1)

Extract

The haemolytic activity of live Trichomonas vaginalis organisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 μm filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37 °C. Treatment of trichomonads with metronidazole reduced levels of haemolysis by > 50%. Maximal haemolysis occurred at the pH range of the vagina during trichomoniasis. N-μ-tosyl-L-lysyl-chloromethyl ketone and iodoacetamide, inhibitors of trichomonad cysteine proteinases, reduced the haemolytic activity of live parasites.

Copyright

References

Hide All
Alderete, J. F., Demeš, P., Gombošova, A., Valent, M., Fabušova, M., Janoška, A., Štefanović, J. & Arroyo, R. (1988). Specific parasitism of purified vaginal epithelial cells by Trichomonas vaginalis. Infection and Immunity 56, 2558–62.
Alderete, J. F., Demeš, P., Gombošova, A., Valent, M., Janoška, A., Fabušova, H., Kasmala, L., Garza, G. E. & Metcalfe, E. C. (1987). Phenotypes and protein/epitope phenotypic variation among fresh isolates of Trichomonas vaginalis. Infection and Immunity 55, 1037–41.
Alderete, J. F. & Garza, G. E. (1985). Specific nature of Trichomonas vaginalis parasitism of host cell surfaces. Infection and Immunity 50, 701–8.
Alderete, J. F. & Garza, G. E. (1988). Identification and properties of Trichomonas vaginalis proteins involved in cytadherence. Infection and Immunity 56, 2833.
Alderete, J. F., Kasmala, L., Metcalfe, E. & Garza, G. E. (1986 a). Phenotypic variation and diversity among Trichomonas vaginalis and correlation of phenotype with trichomonal virulence determinants. Infection and Immunity 53, 285–93.
Alderete, J. F. & Pearlman, E. (1984). Pathogenic Trichomonas vaginalis cytotoxicity to cell culture monolayers. British Journal of Venereal Disease 60, 99105.
Alderete, J. F., Suprun-Brown, L. & Kasmala, L. (1986 b). Monoclonal antibody to a major surface glycoprotein immunogen differentiates isolates and subpopulations of Trichomonas vaginalis. Infection and Immunity 52, 70–5.
Arroyo, R. & Alderete, J. F. (1989). Trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. Infection and Immunity 57, 2991–7.
Brown, M. T. (1972). Trichomoniasis. Practitioner 209, 639–44.
Cohen, M. S., Black, J. R., Proter, R. A. & Sparling, D. F. (1984). Host defenses and vaginal mucosa. In Bacterial Vaginosis, (ed. Mardh, P. & Taylor Robinson, D.) pp. 1322. Stockholm: Almquist and Wiksell.
Coombs, G. H. & North, M. J. (1983). An analysis of the proteinases of Trichomonas vaginalis by acrylamide gel electrophoresis. Parasitology 86, 16.
Diamond, L. S. (1957). The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43, 488–90.
Honigberg, B. M. (1978). Trichomonads of importance in human medicine. In Parasitic Protozoa, vol. 2 (ed. Kreier, J. P.) pp. 275454. New York: Academic Press.
Jirovec, O. & Pertru, M. (1968). Trichomonas vaginalis and Trichomoniasis. Advances in Parasitology 6, 117–88.
Krieger, J. N., Poisson, M. A. & Rein, M. F. (1983). Beta-hemolytic activity of Trichomonas vaginalis correlates with virulence. Infection and Immunity 41, 1291–5.
Krieger, J. N., Ravdin, J. I. & Rein, M. F. (1985). Contact-dependent cytopathogenic mechanisms of Trichomonas vaginalis. Infection and Immunity 50, 778–86.
Nelson, G. L. (1967). Lipid composition of erythrocytes in various mammalian species. Biochemica et Biophysica Acta 44, 221–32.
Peterson, K. M. & Alderete, J. F. (1982). Host plasma proteins on the surface of pathogenic Trichomonas vaginalis. Infection and Immunity 37, 755–62.
Peterson, K. M. & Alderete, J. F. (1984 a). Trichomonas vaginalis is dependent on uptake and degradation of human low density lipoproteins. Journal of Experimental Medicine 160, 1261–72.
Peterson, K. M. & Alderete, J. F. (1984 b). Iron uptake and increased intracellular enzyme activity follow lactoferrin binding by Trichomonas vaginalis receptors. Journal of Experimental Medicine 160, 398410.
Pindak, F., Gardner, J. W. A. & Pindak, M. M. (1986). Growth and cytopathogenicity of Trichomonas vaginalis in tissue cultures. Journal of Clinical Microbiology 23, 672–8.
Rennie, R. P., Freer, J. H. & Arbuthnott, J. P. (1974). The kinetics of erythrocyte lysis by Escherichia coli haemolysin. Journal of Medical Microbiology 7, 189–95.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed