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Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. established through micromanipulation

Published online by Cambridge University Press:  20 July 2006

M. HESS ,
T. KOLBE ,
E. GRABENSTEINER  and
H. PROSL
M. HESS
Affiliation:
Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine, Vienna, Veterinärplatz 1, 1210 Vienna, Austria
T. KOLBE
Affiliation:
Department Biotechnology in Animal Production, Institute for Agrobiotechnology, Konrad Lorenz Straße 20, 3430 Tulln, Austria
E. GRABENSTEINER
Affiliation:
Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine, Vienna, Veterinärplatz 1, 1210 Vienna, Austria
H. PROSL
Affiliation:
Institute of Parasitology and Zoology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210, Vienna, Austria
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Abstract

Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. were established for the first time. Single microbes were successfully isolated from a mixture of micro-organisms obtained from caecal contents of turkeys, using a micromanipulation approach. The cloned parasites were propagated in vitro and maintained through continuous passages multiplying to high numbers. Identification of the protists was done by morphological investigation identifying various forms of each parasite. PCR and partial sequencing of the small subunit rRNA were used to confirm clonality and to determine the relationship of the cloned parasites with known protozoan parasites. The clonal cultures established by this technique will be useful to gain more insight into the biological repertoire of the organisms. In addition, refined infection experiments in different poultry species can now be performed to elucidate the pathological pathways of the respective protozoa.

Keywords

micromanipulationHistomonas meleagridisTetratrichomonas gallinarumBlastocystisclonal culturesribosomal RNA
Type
Research Article
Information
Parasitology , Volume 133 , Issue 5 , November 2006 , pp. 547 - 554
Copyright
2006 Cambridge University Press

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