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Commitment of the malaria parasite Plasmodium falciparum to sexual and asexual development

  • M. C. Bruce (a1), P. Alano (a1), S. Duthie (a1) and R. Carter (a1)


Blood-stage malaria parasites in the vertebrate host can develop either into the asexual, multiplying forms, called schizonts, or into gametocytes, the sexual stages of the parasite. In the present work we studied the differentiation into asexual parasites or gametocytes of the progeny of single, isolated schizonts of the clone 3D7A of Plasinodium falciparum, using monoclonal antibodies specific for the sexual or asexual stages of the parasite. We observed that schizonts obtained from a continuous culture undergoing serial cycles of growth and dilution with fresh red blood cells produced either only gametocytes or only asexual parasites, showing a high degree of commitment to one or the other developmental pathway.

The relative proportion of schizonts which produced gametocytes was very low at low parasite densities in culture, while at high parasite densities a much greater proportion of schizonts produced gametocytes. Nevertheless, at both low and high parasite densities individual schizonts were almost always fully committed to producing only gametocytes or only asexual parasites.



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Carter, R. & Graves, P. M. (1988). Gametocytes. In Malaria – Principles and Practice of Malariology, Vol. 1 (ed. Wernsdorfer, W. H. & McGregor, I.), pp. 253305. Edinburgh: Churchill Livingstone.
Carter, R., Graves, P. M., Creasey, A., Byrne, K., Read, D., Alano, P. & Fenton, B. (1989). Plasmodium falciparum: an abundant stage-specific protein expressed during early gametocyte development. Experimental Parasitology 69, 140–9.
Carter, R. & Miller, L. H. (1979). Evidence for environmental modulation of gametocytogenesis in Plasmodium falciparum in continuous culture. Bulletin of the World Health Organization 57 (Suppl. 1), 3752.
Cornelissen, A. W. C. A. & Walliker, D. (1985). Gametocyte development of Plasmodium chabaudi in mice and rats: evidence for host induction of gametocytogenesis. Zeitschrift für Parasitenkunde 7, 297303.
Fenton, B., Clark, J. T., Wilson, C. F., McBride, J. S. & Walliker, D. (1989). Polymorphism of a 35–48 kDa Plasmodium falciparum merozoite surface antigen. Molecular and Biochemical Parasitology 34, 7986.
Garnham, P. C. C. (1966). Malaria Parasites and Other Haemosporidia. Oxford: Blackwell Scientific Publications.
Inselburg, J. (1983). Gametocyte formation by the progeny of single Plasmodium falciparum schizonts. Journal of Parasitology 69, 584–91.
Mons, B. (1986). Intraerythrocytic Differentiation of Plasmodium berghei. Dordrecht: ICG.
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–7.
Walliker, D., Quakyi, I. A., Wellems, T. E., McCutchan, T. F., Szarfman, A., London, W. T., Corcoran, L. M., Burkot, T. R. & Carter, R. (1987). Genetic analysis of the human malaria parasite, Plasmodium falciparum. Science 236, 1661–6.


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Commitment of the malaria parasite Plasmodium falciparum to sexual and asexual development

  • M. C. Bruce (a1), P. Alano (a1), S. Duthie (a1) and R. Carter (a1)


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