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Degenerating exo-erythrocytic forms of Plasmodium berghei in rat liver: an ultrastructural and cytochemical study

Published online by Cambridge University Press:  06 April 2009

P. H. K. Jap ,
J. F. G. M. Meis ,
J. P. Verhave  and
J. H. E. Th. Meuwissen
P. H. K. Jap
Affiliation:
Medical Parasitology, Geert Grooteplein Z 24University of Nijmegen, 6500 HB Nijmegen, The Netherlands
J. F. G. M. Meis
Affiliation:
Department of Cytology and Histology, Geert Grooteplein N 21University of Nijmegen, 6500 HB Nijmegen, The Netherlands
J. P. Verhave
Affiliation:
Department of Cytology and Histology, Geert Grooteplein N 21University of Nijmegen, 6500 HB Nijmegen, The Netherlands
J. H. E. Th. Meuwissen
Affiliation:
Department of Cytology and Histology, Geert Grooteplein N 21University of Nijmegen, 6500 HB Nijmegen, The Netherlands
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Summary

Morphological and cytochemical aspects of the host response to almost mature exo-erythrocytic forms (EEF) of Plasmodium berghei in rat hepatocytes were studied by electron microscopy. Young stages (< 47 h) never evoked a local reaction. Two types of nearly mature EEF (47–51 h) could be distinguished, normal (EEF type I) and those that were the target of infiltrating cells (EEF type II). The latter were in a stage of early or advanced degeneration and generally exhibited increased electron density, especially in the contents of their peripheral vacuoles. Neither type of EEF exhibited detectable enzyme activity, although host cell enzymes, such as peroxidase and 5-nucleotidase, were demonstrable. However, the infected liver cell appeared permeable to ruthenium red whereas non-infected hepatocytes were not. When signs of degeneration were present, as shown by the increasing density of the cytoplasm, loss of enzyme activities occurred. The encompassing mononuclear cells were identified as true monocytes, non-monocyte-derived and monocyte-derived macrophages by their endogenous peroxidase activity. Macrophage filopodia penetrated and cleaved both hepatocyte and parasite cytoplasms. Subsequently, digestion and clearing of the remnants took place. This study clearly demonstrated that a proportion of the intra-hepatocytic EEF was destroyed by macrophages before they were able to mature completely and release their merozoites.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 2 , October 1982 , pp. 263 - 269
Copyright
Copyright © Cambridge University Press 1982

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References

Bainton, D. F. & Farquhab, M. G. (1968). Difference in enzyme content of azurophil and specific granules of polymorphonuclear leucocytes. II. Cytochemistry and electron microscopy of bone marrow cells. Journal of Cell Biology 39, 299–317.Google Scholar
Bentfeld, M. E., Nichols, B. A. & Bainton, D. F. (1977). Ultrastructural localization of peroxidase in leucocytes of rat bone marrow and blood. Anatomical Record 187, 219CrossRefGoogle Scholar
Daems, W. Th. & Brbderoo, P. (1973). Electron microscopical studies on the structure, phagocytic properties and peroxidase activity of resident and exudate peritoneal macrophages in the guinea pig. Zeitschrift für Zellforschung und Mikroskopische Anatomie 144, 247–97.CrossRefGoogle ScholarPubMed
Daems, W. Th., Wisse, E., Brederook, P. & Emeis, J.J.J. (1975). Peroxidatic activity in monocytes and macrophages. In Mononuclear Phagocytes in Immunity, Infection and Pathology, (ed. Van Furth, R.). Oxford: Blackwell Scientific Publications.Google Scholar
Garnham, P. C. C, Bird, R. G., Baker, J. C. & Killick-Kendrick, R. (1969). Electronmicroscope studies on the motile stages of malaria parasites. VII. The fine structure of the merozoites of exo-erythrocytic schizonts of Plasmodium berghei yoelii. Transactions of the Royal Society of Tropical Medicine and Hygiene 63, 328–32.CrossRefGoogle Scholar
Garnham, P. C. C. & Bray, R. S. (1956). The influence of immunity upon the stages (including late exo-erythrocytic schizonts) of mammalian malaria parasites. Revista Brasileira de Malariologia e Doencas Tropicais 8, 152–60.Google Scholar
Golenser, J., Heeren, J., Verhave, J. P., Van Der Kaay, H. J. & Meuwissen, J. H. E. Th. (1977). Crossreactivity with sporozoites, exo-erythrocytic forms and blood schizonts of Plasmodium berghei in indirect fluorescent antibody tests with sera of rats immunized with sporozoites or infected blood. Clinical and Experimental Immunology 29, 43–51.Google ScholarPubMed
Homewood, C. A. (1978). Biochemistry. In Rodent Malaria (ed. R., Killick-Kendrick and W., Peters) pp. 169–211. London: Academic Press.Google Scholar
Luft, J. H. (1964). Electron microscopy of cell extraneous coats as revealed by ruthenium red staining. Journal of Cell Biology 23, 54A–55A.Google Scholar
Meis, J. F. G. M., Verhave, J. P., Jap, P. H. K., Hess, F. & Meuwissen, J. H. E. Th. (1981). An ultrastructural study of developing stages of exoerythrocytic Plasmodium berghei in rat hepatocytes. Parasitology 82, 195–204.CrossRefGoogle ScholarPubMed
Robbins, D., Cotran, R. S. & Fahimi, H. D. (1971). Fine structural cytochemical localization of peroxidase activity in rat peritoneal cells: mononuclear cells, eosinophils and mast cells. Journal of Histochemistry and Cytochemistry 19, 571–5.CrossRefGoogle ScholarPubMed
Shortt, H. E. & Garnham, P. C. C. (1948). The pre-erythrocytic development of Plasmodium cynomolgi and Plasmodium vivax. Transactions of the Royal Society of Tropical Medicine and Hygiene 41, 785–95.CrossRefGoogle ScholarPubMed
Terzakis, J. A., Vanderberg, J. P., Foley, D. & Shustak, S. (1979). Exo-erythrocytic merozoites of Plasmodium berghei in rat hepatic Kupffer cells. Journal of Protozoology 26, 385–9.CrossRefGoogle Scholar
Vanderberg, J. P., Nussenzweig, R. S. & Most, H. (1968). Further studies on the Plasmodium berghei–Anopheles stephensi-Rodent system of mammalian malaria. Journal of Parasitology 54, 1009–16.Google Scholar
Vanderberg, J. P., Nussenzweig, R. S., Most, H. & Orton, C. G. (1968). Protective immunity produced by the injection of X-irradiated sporozoites of Plasmodium berghei. II. Effects of radiation on sporozoites. Journal of Parasitology 54, 1175–80.CrossRefGoogle ScholarPubMed
Wisse, E., Van 'T Noordende, J. M., Van Der Meulen, J. & Daems, W. Th. (1976). The pit cell: Description of a new type of cell occurring in rat liver sinusoids and peripheral blood. Cell and Tissue Research 173, 423–35.Google Scholar
Yoeli, M. & Most, H. (1965). Studies on sporozoite-induced infections of rodent malaria. I. The pre-erythrocytic tissue stage of Plasmodium berghei. The American Journal of Tropical Medicine and Hygiene 14, 700–14.CrossRefGoogle ScholarPubMed