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Differential polyadenylation of ribosomal RNA during post-transcriptional processing in Leishmania

  • S. DECUYPERE (a1) (a2), J. VANDESOMPELE (a3), V. YARDLEY (a4), S. DE DONCKER (a1), T. LAURENT (a1), S. RIJAL (a5), A. LLANOS-CUENTAS (a6), F. CHAPPUIS (a7), J. AREVALO (a6) and J.-C. DUJARDIN (a1)...

Abstract

The protozoan parasite Leishmania belongs to the most ancient eukaryotic lineages and this is reflected in several distinctive biological features, such as eukaryotic polycistronic transcription and RNA trans-splicing. The disclosure of this organism's unusual characteristics leads to a better understanding of the origin and nature of fundamental biological processes in eukaryotes. Here we report another unusual phenomenon as we demonstrate that precursor ribosomal RNA can be extensively polyadenylated during post-transcriptional processing[dagger]. Furthermore, we demonstrate that the degree of precursor rRNA polyadenylation is variable in different strains and in the different life-stages of a strain.

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‘Prins Leopold’ Instituut voor Tropische Geneeskunde, Unit of Molecular Parasitology, Nationalestraat 155, B-2000 Antwerpen, Belgium. Tel: +32 3 2476358. Fax: +32 3 2476359. E-mail: jcdujard@itg.be

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Parasitology
  • ISSN: 0031-1820
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