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The effect of temperature on the development and survival of the infective larvae of Strongyloides ratti Sandground, 1925

Published online by Cambridge University Press:  06 April 2009

J. Barrett
J. Barrett
Affiliation:
The Molteno Institute, University of Cambridge
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Extract

The development of the free-living infective larvae of a homogonic strain Strongyloides ratti is described.

The larvae develop only between 15 and 34 °C. Transfer experiments show the temperature block to be in the preparation for the second moult.

Within the temperature range 15–34 °C, increasing the temperature speeds up the rate of development of all the larval stages equally, the Q10 for development being 2·5.

The maximum percentage development occurs at 20 °C. The percentage development is highest in faeces–peat culture (95% development at 20 °C), whilst the percentage development in charcoal and vermiculite cultures is about the same (75% development at 20 °C.).

Larvae grown on charcoal cultures are larger than those grown on vermiculite, which are larger than those grown on peat. No significant difference was found in the length:oesophagus and length:width ratios or in the variability of larvae grown at different temperatures or on different culture media.

Different worm densities in the cultures of from 2000 to 10000 larvae per g of culture did not affect either the size of the infective larve or the percentage development.

The optimum temperature for survival is 15 °C. Worms grown at 20 °C lived longer than worms grown at any other temperature. There was no evidence of temperature adaptation by the larvae.

The infective larvae are positively thermotactic, and show maximum activity at 37 °C.

I should like to thank my supervisor, Dr Tate, for his advice and encouragement. The work was carried out during the tenure of a Medical Research Council Scholarship.

Type
Research Article
Information
Parasitology , Volume 58 , Issue 3 , August 1968 , pp. 641 - 651
Copyright
Copyright © Cambridge University Press 1968

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References

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