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Effects of temperature on Paramoeba perurans growth in culture and the associated microbial community

Published online by Cambridge University Press:  25 October 2018

O. Benedicenti Open the ORCID record for O. Benedicenti [Opens in a new window] ,
C. J. Secombes  and
C. Collins
O. Benedicenti*
Affiliation:
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, UK Marine Scotland Science Marine Laboratory, 375 Victoria Rd, Aberdeen AB11 9DB, UK
C. J. Secombes
Affiliation:
Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, UK
C. Collins*
Affiliation:
Marine Scotland Science Marine Laboratory, 375 Victoria Rd, Aberdeen AB11 9DB, UK
*
Author for correspondence: O. Benedicenti, E-mail: ottavia.benedicenti@gmail.com and C. Collins, E-mail: Catherine.Collins@gov.scot
Author for correspondence: O. Benedicenti, E-mail: ottavia.benedicenti@gmail.com and C. Collins, E-mail: Catherine.Collins@gov.scot
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Abstract

Population growth, in vitro, of three Paramoeba perurans cultures, one polyclonal (G) and two clonal (B8, CE6, derived from G), previously shown to differ in virulence (B8 > G > CE6), was compared at 10 and 15 °C. B8 showed a significantly higher increase in attached and in suspended amoebae over time at 15 and 10 °C, respectively. CE6 and G also had significantly higher numbers of suspended amoebae at 10 °C compared with 15 °C at experiment termination. However, in contrast to B8, numbers of attached amoebae were significantly higher at 10 °C in CE6 but showed a similar trend in G at the end of the experiment. Numbers of both suspended and attached amoebae were lower in B8 compared with CE6 and G. Significant differences in bacterial community composition and/or relative abundances were found, between cultures, between temperatures and between the same culture with and without amoebae, based on 16S rRNA Illumina MiSeq sequencing. Bacterial diversity was lower in B8 and CE6 compared with G, possibly reflecting selection during clonal isolation. The results indicate that polyclonal P. perurans populations may contain amoebae displaying different growth dynamics. Further studies are required to determine if these differences are linked to differences seen in the bacterial communities.

Keywords

Amoebic gill diseaseParamoeba perurans16S rRNAtemperature
Type
Research Article
Information
Parasitology , Volume 146 , Issue 4 , April 2019 , pp. 533 - 542
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Copyright
Copyright © Cambridge University Press 2018 

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