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Excretion of fluorescent substrates of mammalian multidrug resistance-associated protein (MRP) in the Schistosoma mansoni excretory system

Published online by Cambridge University Press:  19 January 2004

H. SATO ,
J. R. KUSEL  and
J. THORNHILL
H. SATO
Affiliation:
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, Scotland present address: Department of Parasitology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan. Fax: +81 172 39 5045. E-mail: sato7dp4@cc.hirosaki-u.ac.jp
J. R. KUSEL
Affiliation:
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, Scotland
J. THORNHILL
Affiliation:
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, Scotland
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Abstract

The protonephridium of platyhelminths including Schistosoma mansoni plays a pivotal role in their survival by excretion of metabolic wastes as well as xenobiotics, and can be revealed in the living adult parasite by certain fluorescent compounds which are concentrated in excretory tubules and collecting ducts. To determine the presence of the multidrug resistance-associated protein (MRP) as a possible transporter in protonephridial epithelium, adult schistosomes were exposed to a fluorescent Ca2+ indicator, fluo-3 acetyloxymethyl ester, which is a potential substrate of mammalian MRP. Specific fluorescence related to fluo-3/Ca2+ chelate delineated the whole length of the protonephridial system. Simultaneously, a fluorescent substance was accumulated in the posterior part of collecting ducts and the excretory bladder. Similarly, when other fluorogenic substrates for mammalian MRP such as monoclorobimane, fluorescein diacetate, and 5(6)-carboxyfluorescein diacetate were applied to adult schistosomes, these fluorescent markers were observed in the excretory tubules through to the excretory bladder. The excretory system of mechanically-transformed schistosomula was not labelled with any of these 4 fluorescent markers. These findings suggest that the protonephridial epithelium of adult schistosomes, but not schistosomula, might express the homologue of the mammalian MRP transporting organic anionic conjugates with glutathione, glucuronate or sulphate as well as unconjugated amphiphilic organic anions.

Keywords

Schistosoma mansoniplatyhelminthprotonephridiummultidrug-resistance-associated protein (MRP)fluo-3Ca2+
Type
Research Article
Information
Parasitology , Volume 128 , Issue 1 , January 2004 , pp. 43 - 52
Copyright
2004 Cambridge University Press

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