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The excretory system of nematodes: structure and ultrastructure of the excretory system of Panagrellus redivivus, Ditylenchus myceliophagus with some observations on D. dipsaci and Heterodera rostochiensis

Published online by Cambridge University Press:  06 April 2009

H. K. Narang
Affiliation:
Department of Zoology, University of Newcastle upon Tyne*

Extract

The structure and ultrastructure of the excretory system of Panagrellus redivivus, Ditylenchus myceliophagus, D. dipsaci and the second-stage larvae of the Heterodera rostochiensis are described. Panagrellus has two lateral ducts, a median duct and a gland. The two lateral ducts are joined over the anterior part of the oesophageal bulb to form a loop. Anteriorly this loop continues as a median duct, which opens to the exterior in an excretory pit, part of the system lies free in the pseudocoelom. The lumen of the lateral excretory duct and the greater part of the median duct is surrounded by capitate tubules, which open into the lumen of the excretory duct. Capitate tubules develop by the invagination of the hypodermal membranes and lie embedded in thick cytoplasm surrounded by mitochondria. Ensheathed in cell membranes the excretory duct forms an anastomosis in the loop and at three other places along the length of each lateral excretory duct. At these regions there are nerve endings. The excretory gland opens posteriorly into the loop and is full of Golgi complexes, endoplasmic reticulum and secretory granules.

In the system of Ditylenchus and the second-stage larva of Heterodera the right lateral duct is absent while the left lateral excretory duct continues anteriorly past the median excretory duct; a true loop is not formed. The median excretory duct lies embedded almost throughout its length in the excretory gland. Capitate tubules are present along the length of the lateral excretory duct, but, unlike Panagrellus, are not present around the median excretory duct. Anastomosis is not formed in the excretory ducts. In other respects the system of the species studied is similar to that of Panagrellus.

The capitate tubules form a system of fine microtubules. Their ultrastructure and, particularly, their association with large numbers of mitochondria, is very reminiscent of structures thought to be concerned with an active transport role, related to ionic and osmoregulation in other phyla.

The excretory system is almost certainly hypodermal in origin. Differences between the morphology and relations of the median duct of Enoplus (Narang, 1970), on the one hand, and that of the other forms studied suggest that these ducts may have different origins: the presence of separate nuclei in the lateral duct and gland portions of the system would be consistent with this view. It is considered that the two parts may have originated separately and have become joined during evolution. It is also shown that the excretory system of Ditylenchus cannot be derived either from an H-shaped system or from a primitive gland system.

I wish to thank Professor C. Ellenby and Mr L. Smith for their helpful criticism.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Alicata, J. E. (1935). Early developmental stages of nematodes occurring in swine. U.S. Department of Agricultural Technical Bulletins, no. 489, pp. 196.Google Scholar
Allen, M. W. (1960). Alimentary canal, excretory and nervous systems. In Nematology (ed. Sasser, J. N. and Jenkins, W. R.), pp. 136–9. Chapel Hill: The University of North Carolina Press.Google Scholar
Beam, H. W. & Anderson, E. (1956). Ultrastructure of Golgi apparatus, mitochondria and endoplasmic reticulum. Proceedings of Iowa Academy of Science 63, 686–92.Google Scholar
Chitwood, M. B. & Chitwood, B. G. (1950). An Introduction to Nematology, section I, chap. II and IX. Babylon Press, Babylon, New York.Google Scholar
Cobb, N. A. (1890). Oxyuris-larvae hatched in the human stomach under normal conditions. Proceedings of Linnean Society of New South Wales 5, 168–85.CrossRefGoogle Scholar
Dalton, A. J. (1951). Structural detail of some of the epithelial cell types in kidney of the mouse as revealed by the electron microscope. Journal of National Cancer Institute 11, 1163–85.Google ScholarPubMed
Davey, K. G. & Kan, S. P. (1968). Moulting in parasitic nematode Phocanema decipiens. IV. Ecdysis and its control. Canadian Journal of Zoology 46, 893–9.CrossRefGoogle ScholarPubMed
Evans, A. A. F. & Fisher, J. M. (1970). The excretory system of three Ditylenchus spp. Journal of Nematology 2, 260–4.Google ScholarPubMed
Fawcett, D. W. (1966). The Cell: Its Organelles and Inclusions. Philadelphia and London: W. B. Saunders Co.Google Scholar
Golovin, E. P. (1902). Observations on Nematodes. II. Excretory apparatus. [Russian text.] Uchenye/Zapiski Imperatorskago Kazanskago Universiteta, pp. 1120.Google Scholar
Goodey, T. (1951). Soil and Freshwater Nematodes. London: Methuen and Co. Ltd.CrossRefGoogle Scholar
Lee, D. L. (1970). The fine structure of the excretory system in adult Nippostrongylus brasiliensis (Nematoda) and a suggested function for the excretory gland. Tissue and Cell 2, 225–31.CrossRefGoogle Scholar
Mueller, J. F. (1927). The excretory system of Anisakis simplex. Zeitschrift für Zellforschung und mikroscopische Anatomie 5, 495504.CrossRefGoogle Scholar
Narang, H. K. (1970). The excretory system of nematodes: Structure and ultrastructure of the excretory system of Enoplus brevis (Bastian). Nematologica 16, 517–22.CrossRefGoogle Scholar
Pantin, C. F. A. (1962). Notes on Microscopical Techniques for Zoologists. Cambridge University Press.Google Scholar
Pease, D. C. & Baker, R. F. (1950). Electron microscopy of the kidney. American Journal of Anatomy 87, 349–90.CrossRefGoogle ScholarPubMed
Rhodin, J. (1958). Anatomy of kidney tubules. International Review of Cytology 7, 485534.CrossRefGoogle Scholar
Roggen, D. R., Raski, D. J. & Jones, N. O. (1966). Cilia in nematode sensory organs. Science 152, 515–16.CrossRefGoogle ScholarPubMed
Sjostrand, F. S. & Rhodin, J. (1953). The ultrastructure of the provisional convoluted tubules of the mouse kidney as revealed by high resolution electron microscopy. Experimental Cell Research 4, 436–56.CrossRefGoogle Scholar
Smith, L. (1965). The excretory system of Panagrellus redivivus (T. Goodey, 1945). Comparative Biochemistry and Physiology 15, 8992.CrossRefGoogle ScholarPubMed
Steiner, G. (1919). Untersuchungen über den allgemeinen Bauplan de Nematodenkörpers, pp. 196.Google Scholar
Steiner, G. (1920). Betrachtungen zur Frage des Verwandschaftsverhaltnisses der Rotatorien und Nematoden. Festschrift Zshokke, pp. 116.Google Scholar
Stewart, F. H. (1916). On the life history of Ascaris lumbricoides. British Medical Journal 2, 57.CrossRefGoogle ScholarPubMed
Waddell, A. H. (1968). The excretory system of the kidney worm Stephanurus dentatus (Nematoda). Parasitology 58, 907–19.CrossRefGoogle Scholar
Wigglesworth, V. B. & Salpeter, M. M. (1962). Histology of the malpighian tubules in Rhodium prolixus Stal (Hemiptera). Journal of Insect Physiology 8, 299307.CrossRefGoogle Scholar
Yuen, P. H. (1968). Electron microscopical studies on the anterior end of Panagrellus silusiae (Rhabditidae). Nematologica 14, 554–64.CrossRefGoogle Scholar