Skip to main content
×
Home
    • Aa
    • Aa

Extraction of microsporidial DNA from modified trichrome-stained clinical slides and subsequent species identification using PCR sequencing

  • K. S. CHAN (a1) and T. H. KOH (a1)
Abstract
SUMMARY

Molecular techniques involving polymerase chain reaction (PCR) and sequencing provide a relatively simple and objective means of identifying microsporidia to species level. We modified previously described methods of DNA extraction and PCR conditions for identification of microsporidia from museum slides, clinical specimens and environmental samples and successfully identified Vittaforma corneae in 11 out of 13 cases of microsporidial infection from used trichrome-stained slides of corneal scrapings from HIV-negative patients with keratoconjunctivitis.

Copyright
Corresponding author
*Corresponding author. Tel: +65 91804878/+65 63214505. Fax: +65 6222 6826. E-mail address: chankiansing@yahoo.com.sg
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

M. Hyliš , J. Weiser , M. Obornik and J. Vavra (2005). DNA isolation from museum and type collection slides of microsporidia. Journal of Invertebrate Pathology 88, 257260.

J. Joseph , S. Sharma , S. I. Murthy , P. V. Krishna , P. Garg , R. Nutheti , J. Kenneth and D. Balasubramanian (2006). Microsporidial keratitis in India: 16S rRNA gene-based PCR assay for diagnosis and species identification of microsporidia in clinical samples. Investigative Ophthalmology Visual Science 47, 44684473.

R. Weber , D. A. Schwartz and P. Deplazes (1999). Laboratory diagnosis of microsporidiosis. In The Microsporidia and Microsporidiosis (ed. M. Wittner and L. M. Weiss ), pp. 315362. ASM Press, Washington, DC.

L. M. Weiss and C. R. Vossbrinck (1999). Molecular biology, molecular phylogeny and molecular diagnostic approaches to the microsporidia. In The Microsporidia and Microsporidiosis (ed. M. Wittner and L. M. Weiss ), pp. 129171. ASM Press, Washington, DC.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 1
Total number of PDF views: 3 *
Loading metrics...

Abstract views

Total abstract views: 58 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 23rd May 2017. This data will be updated every 24 hours.