Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 2
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Wu, Jian and Norton, Pamela A. 1996. Animal Models of Liver Fibrosis. Scandinavian Journal of Gastroenterology, Vol. 31, Issue. 12, p. 1137.

    Kresina, Thomas F He, Qing Esposti, Silvia Degli and Zern, Mark A 1994. Gene expression of transforming growth factor β1 and extracellular matrix proteins in murine Schistosoma mansoni infection. Gastroenterology, Vol. 107, Issue. 3, p. 773.


Hepatic fibrosis and gene expression changes induced by praziquantel treatment during immune modulation of Schistosoma japonicum infection

  • T. F. Kresina (a1), Qing He (a1), S. Degli Esposti (a2) and M. A. Zern (a3)
  • DOI:
  • Published online: 01 April 2009

In the present study fibrogenic gene expression was determined in murine Schistosoma japonicum infection during the progression of immune modulation of infection and following chemotherapy during the course of immune modulation. Histomorphometric analysis of granuloma size and collagen deposition revealed peak granuloma size in acute infection (5 weeks) and peak hepatic collagen content at 16 weeks of infection. Peak Type I collagen gene expression was concomitant with TGF-β1 gene expression at 8–11 weeks. Chemotherapy during either acute (9 weeks) or chronic (24, 28 weeks) infection resulted in increased collagen deposition and increased gene expression of Type I collagen and TGF-β1. However, chemotherapy at 14–16 weeks resulted in decreased levels of TGF-β1 gene expression and essentially minimal change in Type I collagen deposition and gene expression. These data indicate that chemotherapy of schistosomiasis japonica does not reverse hepatic fibrogenesis when administered in acute infection – when granuloma size is maximal – or in chronic infection. However, a beneficial effect on hepatic fibrogenesis is seen when chemotherapy is administered at 14–16 weeks post-infection, a time of decreasing granuloma size and maximal hepatic collagen content. Thus the ability to reverse schistosomal-induced hepatic fibrogenesis by chemotherapy may depend on disease stage.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

M. Barral-Netto , A. Barral , C. E. Brownell , Y. A. W. Skeiky , L. Ellingsworth , D. R. Twardzik & S. Reed (1992). Transforming growth factor B production in Leishmanial infection: a parasite escape mechanism. Science 257, 545–8.

J. M. Chirgwin , A. E. Przybyla , R. J. Macdonald & W. J. Rutter (1979). Isolation of biologically active ribonucleic acid from sources enriched with ribonuclease. Biochemistry 18, 5294–9.

M. J. Czaja , F. R. Weiner , K. C. Flanders , M. A. Giambrone , R. Wind , L. Biempica & M. A. Zern (1989). In vitro and in vivo association of transforming growth factor-B1 with hepatic fibrosis. Journal of Cell Biology 108, 2477–82.

R. Derynck , J. A. Jarrett , E. Y. Chey , D. H. Eaton , J. R. Bell , R. K. Assoian , A. B. Roberts , M. B. Sporn & D. V. Goelddel (1985). Human transforming growth factor-beta cDNA sequence and expression in tumor cell lines. Nature, London316, 701–5.

M. A. Dunn & R. Kamel (1981). Hepatic schistosomiasis. Hepatology 1, 653–61.

C. Genovese , D. Rowe & B. Kream (1984). Construction of DNA sequences complementary to rat a2 mRNA and their use in studying the regulation of type I collagen synthesis by 1–25 dihydroxy vitamin D. Biochemistry 23, 6210–16.

C. C. Howe , R. Kath , M. L. Mancianti , M. Herlyn , S. Mueller & V. Cristofalo (1990). Expression and structure of human SPARC transcripts. Experimental Cell Research 188, 185–91.

J. G. Kehrl , A. Taylor , S. J. Kim & A. S. Fauci (1991). Transforming growth factor-beta is a potent negative regulator of human lymphocytes. Annals of the New York Academy of Sciences 628, 345–50.

T. F. Lane , E. H. Sage (1990). Functional mapping of SPARC: peptides from two distinct Ca++ binding sites modulate cell shape. Journal of Cell Biology 111, 3065–76.

G. R. Olds , A. Griffin & T. F. Kresina (1985). Dynamics of collagen accumulation and polymorphism in murine Schistosoma japonicum. Gastroenterology 89, 617–24.

G. R. Olds & T. F. Kresina (1985). Network interactions in Schistosoma japonicum infection. Identification and characterization of a serologically distinct immunoregulatory auto-antiidiotypic antibody population. Journal of Clinical Investigation 76, 2338–47.

G. R. Olds & T. F. Kresina (1989). Immunoregulation of hepatic fibrosis in murine schistosomiasis japonica. Journal of Infectious Diseases 159, 798801.

J. E. Schwarzbauer , J. W. Tamkun , I. R. Lemischka & R. O. Hynes (1983). Three different fibronectin mRNAs arise by alternative splicing within the coding region. Cell 35, 421–31.

A. Sher , R. T. Gazzinelli , I. P. Oswals , M. Clerici , M. Kullberg , E. J. Pearce , J. A. Berzofsky , T. R. Mosmann , S. L. James , H. C. Morse & G. M. Shearer (1992). Role of T-cell derived cytokines in the down regulation of immune responses in parasitic and retroviral infection. Immunological Reviews 127, 183204.

S. L. Swain , L. M. Bradley , M. Croft , S. Tonkongy , F. Atking , A. Weinberg , D. D. Duncan , S. M. Hedrick , R. W. Dutton & G. Juston (1991). Helper T-cell subsets: phenotype, function and the role of lymphokines in regulating their development. Immunological Reviews 123, 115–29.

S. M. Wahl , N. McCartney-Francis & S. E. Mergenhagen (1989). Inflammatory and immunomodulatory roles of TGF-β. Immunology Today 10, 258–61.

S.-Y. Wang & L. J. Gudas (1983). Isolation of cDNA clones specific for collagen IV and laminin from mouse teratocarcinoma cells. Proceedings of the National Academy of Sciences, USA80, 5880–8.

F. R. Wiener , M. J. Czaja , M. A. Giambrone , S. Takahash , L. Biempica & M. A. Zern (1987). Transcriptional and post-transcriptional effects of dexamethasone on albumin and procollagen messenger RNAs in murine schistosomiasis. Biochemistry 26, 1557–62.

G. C. Wong , P. A. Temple , A. C. Leary , J. S. Witekgiannolti , Y. Yu-Chung , A. B. Ciarlette , M. Chung , R. Murthop Kriz , R. J. Kaufman , R. M. Henick , S. C. Clark , N. Yanau , H. Yokota , M. Yamaha , M. Saito , K. Motoyoski & E. Takaka (1987). Human CSF-1 molecular cloning and expression of 4-lab cDNA encoding the human urinary protein. Science 235, 1504–8.

K. M. Yamada (1991). Fibronectin and other cell interactive glycoproteins. In Cell Biology of the Extracellular Matrix (ed. E. D. Hay ). 2nd Edn, pp. 111146. New York: Plenum Press.

M. A. Zern , P. R. Chakraborty , N. Ruiz-Opazo , S. H. Yap & D. A. Shakritz (1983). Development and use of a rat albumin cDNA clone to evaluate the effect of chronic ethanol administration of hepatic protein synthesis. Hepatology 3, 317–22.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *