Skip to main content
    • Aa
    • Aa

Hypoxanthine: a low molecular weight factor essential for growth of erythrocytic Plasmodium falciparum in a serum-free medium

  • H. Asahi (a1), T. Kanazawa (a1), Y. Kajihara (a2), K. Takahashi (a2) and T. Takahashi (a3)...

A low molecular weight factor in a basal medium essential for erythrocytic Plasmodium falciparum development in a serum-free medium using a cell growth-promoting factor derived from adult bovine serum was detected. The factor was hypoxanthine. The optimal hypoxanthine concentration for parasite growth was between 15 and 120 μM. The contribution of hypoxanthine to increased parasite growth was clearly evident in cultures on day 4. Among various low molecular weight supplements tested, adenine, adenosine, AMP, ATP, cyclic AMP, guanine, guanosine, inosine, inosine mono-phosphate, xanthine, NAD, NADH, NADP, NADPH and deoxyguanosine triphosphate showed a similar effect to that of hypoxanthine in the serum-free culture system. On the other hand, the addition of uric acid, FAD, thymidine, uridine, orotic acid, deoxythymidine triphosphate, deoxycytidine triphosphate, deoxyadenosine triphosphate, ribose-1-phosphate, or ethanolamine was not beneficial to the parasite growth. The results presented here will not only be of practical value, but will provide important information about the developmental requirements of the parasite.

Corresponding author
*Corresponding author. Tel: +3 5285 1111. Fax: +3 5285 1150.
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

A. A. Divo , Waa J. A. Vande , J. R. Campbell & J. B. Jensen (1985). Isolation and cultivation of Plasmodium falciparum using adult bovine serum. Journal of Parasitology 71, 504–9.

N. Elabbadi , M. L. Ancelin & H. J. Vial (1992). Use of radioactive ethanolamine incorporation into phospholipids to assess in vitro antimalarial activity by the semiautomated microdilution technique. Antimicrobial Agents and Chemotherapy 36, 50–5.

P. Grellier , D. Rigomier , V. Clavey , J.-C. Fruchart & J. Schrevel (1991). Lipid traffic between high density lipoproteins and Plasmodium falciparum-infected red blood cells. Journal of Cell Biology 112, 267–77.

W. E. Gutteridge & P. I. Trigg (1970). Incorporation of radioactive precursors into DNA and RNA of Plasmodium knowlesi in vitro. Journal of Protozoology 17, 8996.

T. Kudo , R. Morishita , (Seno) R. Suzuki & T. Tachibana (1987). A great improvement of fusion efficiency in mouse B cell hybridoma production by use of the new culture medium, GIT. Tohoku Journal of Experimental Medicine 153, 5566.

G. Kugler (1978). A column chromatographic method for determination of plasma and erythrocyte levels of inosine and hypoxanthine. Analytical Biochemistry 90, 204–10.

A. Lingnau , G. Margos , W. A. Maier & H. M. Seitz (1993). Serum-free cultivation of Plasmodium falciparum gametocytes in vitro. Parasitology Research 79, 378–84.

P. Reyes , P. K. Rathod , D. J. Sanchez , J. E. K. Mrema , K. H. Rieckmann & H-G. Heidrich (1982). Enzymes of purine and pyrimidine metabolism from the human malaria parasite, Plasmodium falciparum. Molecular and Biochemical Parasitology 5, 275–90.

W. Trager & J. B. Jensen (1976). Human malaria parasites in continuous culture. Science 193, 673–5.

J. W. Zolg , A. J. Macleod , I.H. Dickson & J. G. Scaife (1982). Plasmodium falciparum: modifications of the in vitro culture conditions improving parasite yields. Journal of Parasitology 68, 1072–80.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 7 *
Loading metrics...

Abstract views

Total abstract views: 67 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 28th May 2017. This data will be updated every 24 hours.