Skip to main content

Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei

  • G. A. M. Cross (a1)

Soluble glycoproteins have been purified from a series of clones of Trypanosoma brucei 427. Each clone yielded a characteristic predominant glycoprotein which induced clone-specific immunity to trypanosome infection in mice. These glycoproteins were shown by specific labelling and enzyme digestion of cells to be the major components of the trypanosome surface coat. Each glycoprotein consisted of a single polypeptide chain having an apparent molecular weight of 65000 (as measured by SDS-polyacrylamide gel electrophoresis) and containing around 600 amino acid and 20 monosaccharide residues. Preliminary structural studies indicated large changes in amino acid sequence dispersed over a considerable length of the polypeptide chain. Proteolytic activity was demonstrated in semi-purified trypanosome extracts, providing one reason for the heterogeneity sometimes observed in surface glycoprotein antigen preparations.

Hide All
Allsopp B. A. & Njogu A. R. (1974). Monosaccharide composition of the surface glyco protein antigens of Trypanosoma brucei. Parasitology 69, 271–81.
Allsopp B. A., Njogu A. R. & Humphryes K. C. (1971). Nature and location of Trypanosoma brucei subgroup exoantigen and its relationship to 4s antigen. Experimental Parasitology 29, 271–84.
Bretscher M. S. (1971 a). Human erythrocyte membranes: specific labelling of surface proteins. Journal of Molecular Biology 58, 775–81.
Bretscher M. S. (1971 b). A major protein which spans the human erythrocyte membrane. Journal of Molecular Biology 59, 351–7.
Bretscher M. S. (1971 c). Major human erythrocyte glycoprotein spans the cell membrane. Nature, New Biology 231, 229–32.
Bretscher M. S. (1973 a). On labelling membranes. Nature, New Biology 245, 116–7.
Bretscher M. S. (1973 b). Membrane structure: some general principles. Science 181, 622–9.
Cross G. A. M. & Manning J. C. (1973). Cultivation of Trypanosoma brucei sspp. in semidefined and defined media. Parasitology 67, 315–31.
De Raadt P. (1974). Immunity and antigenic variation: clinical observations suggestive of immune phenomena in African trypanosomiasis. In Ciba Foundation Symposium 20 (new series): Trypanosomiasis and Leishmaniasis with special reference to Chagas' disease, pp.199224. Amsterdam: Associated Scientific Publishers.
Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A. & Smith F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–6.
Gray A. R. (1965). Antigenic variation in a strain of Trypanosoma brucei transmitted by Glossina morsitans and G. palpalis. Journal of General Microbiology 41, 195214.
Jullano R. L. & Behar-Bannelier M. (1975). An evaluation of techniques for labelling the surface proteins of cultured mammalian cells. Biochemica et Biophysica Acta 375, 249–67.
Lanham S. M. (1968). Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers. Nature, London 218, 1273–4.
Lanham S. M. & Godfrey D. G. (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Experimental Parasitology 28, 521–34.
Lanham S. M. & Taylor A. E. R. (1972). Some properties of the immunogens (protective antigens) of a single variant of Trypanosoma brucei brucei. Journal of General Microbiology 72, 101–16.
Le Page R. W. F. (1967). Studies on the variable antigens of Trypanosoma brucei. Transactions of the Royal Society of Tropical Medicine and Hygiene 61, 139–40.
Le Page R. W. F. (1968 a). Further studies on the variable antigens of T. brucei. Transactions of the Royal Society of Tropical Medicine and Hygiene 62, 131.
Le Page R. W. F. (1968 b). Antigenic variation in Trypanosoma brucei. Thesis submitted for the degree of Ph.D., University of Cambridge.
Lowry O. H., Rosebrough N. J., Farr A. L. & Randall R. J. (1951). Protein measure ment with the folin phenol reagent. Journal of Biological Chemistry 193, 265–75.
Luckins A. G. (1972). Effects of X-irradiation and cortisone treatment of albino rats on infections with Brucei-complex trypanosomes. Transactions of the Royal Society of Tropical Medicine and Hygiene 66, 130–9.
Morgan J. F., Morton H. J. & Parker R. C. (1950). Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proceedings of the Society for Experi mental Medicine 73, 18.
Schmidt-Ullrich R., Knüfermann H. & Wallach D. F. H. (1973). The reaction of 1-dimethylaminonaphthalene-5-sulphonyl chloride (DANSCI) with erythrocyte mem branes. Biochimica et Biophysica Acta 307, 353–65.
Seed J. R. (1972). Trypanosoma gambiense and T. equiperdum: characterization of variant specific antigens. Experimental Parasitology 31, 98108.
Seed J. R. (1974). Antigens and antigenic variability of the African trypanosomes. Journal of Protozoology 21, 639–46.
Vickernan K. (1969). On the surface coat and flagellar adhesion in trypanosomes. Journal of Cell Science 5, 163–93.
Vickerman K. (1971). Morphological and physiological considerations of extracellular blood protozoa. In Ecology and Physiology of Parasites: a Symposium (ed. Fallis A. M.), pp. 5891. Toronto: University Press.
Vickerman K. (1974). Antigenic variation in African trypariosomes. In Ciba Foundation Symposium 25 (new series): Parasites in the immunized host: mechanisms of survival, pp. 5380. Amsterdam: Associated Scientific Publishers.
Vickerman K. & Luckins A. G. (1969). Localization of variable antigens in the surface coat of Trypanosoma brucei using ferritin-conjugated antibody. Nature, London 224, 1125–7.
Wallach D. F. H. (1972). The dispositions of proteins in the plasma membranes of animal cells: analytical approaches using controlled peptidolysis and protein labels. Biochimica et Biophysica Acta 265, 6183.
Weber K. & Osborn M. (1969). The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244, 4406–12.
Wright K. A. & Hales H. (1970). Cytochemistry of the pellicle of bloodstream forms of Trypanosoma (trypanozoon) brucei. Journal of Parasitology 56, 671–83.
Zwaal R. F. A., Roelofson B. & Colley C. M. (1973). Localization of red cell membrane constituents. Biochimica et Biophysica Acta 300, 159–82.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 49 *
Loading metrics...

Abstract views

Total abstract views: 861 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 12th December 2017. This data will be updated every 24 hours.