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Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice

Published online by Cambridge University Press:  05 October 2004

V. R. A. PEREIRA ,
V. M. B. LORENA ,
A. P. GALVAO DA SILVA ,
E. M. COUTINHO ,
E. D. SILVA ,
A. G. P. FERREIRA ,
P. MIRANDA ,
M. A. KRIEGER ,
S. GOLDENBERG  and
M. B. P. SOARES
...Show all authors
V. R. A. PEREIRA
Affiliation:
Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil
V. M. B. LORENA
Affiliation:
Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil
A. P. GALVAO DA SILVA
Affiliation:
Universidade Federal de Pernambuco, Recife, PE, Brazil
E. M. COUTINHO
Affiliation:
Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil
E. D. SILVA
Affiliation:
Bio-Manguinhos/FIOCRUZ, Salvador, BA, Brazil
A. G. P. FERREIRA
Affiliation:
Bio-Manguinhos/FIOCRUZ, Salvador, BA, Brazil
P. MIRANDA
Affiliation:
Universidade Federal de Pernambuco, Recife, PE, Brazil
M. A. KRIEGER
Affiliation:
Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ, Brazil Instituto de Biologia Molecular do Paraná/IBMP, Curitiba, PR
S. GOLDENBERG
Affiliation:
Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ, Brazil Instituto de Biologia Molecular do Paraná/IBMP, Curitiba, PR
M. B. P. SOARES
Affiliation:
Centro de Pesquisas Gonçalo Moniz/FIOCRUZ, Salvador, BA, Brazil
R. CORREA-OLIVEIRA
Affiliation:
Centro de Pesquisas René Rachou/FIOCRUZ, Belo Horizonte, MG, Brazil
Y. M. GOMES
Affiliation:
Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil
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Abstract

In previous studies, we demonstrated that CRA and FRA recombinant proteins, used for diagnosis of Chagas' disease, elicited a humoral immune response in susceptible and resistant mice. To understand better the immune response to these proteins, we have evaluated, the cellular immune response in CRA- and in FRA-immunized BALB/c and C57BL/6 mice. A specific cellular lymphoproliferative response was observed in both strains of mice. Spleen cell cultures mainly from CRA-immunized C57BL/6 and FRA-immunized BALB/c mice produced high levels of IFN-γ, indicating the induction of a Type 1 immune response. Regarding the T cell subsets, CD4+ T cells were the major source of IFN-γ in CRA- and FRA-immunized mice. These results suggest that CRA and FRA are important immunogens in inducing a Type 1 immune response and that they may be considered as potential vaccine antigens.

Keywords

recombinant proteinsimmune cellular responseTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 129 , Issue 5 , November 2004 , pp. 563 - 570
Copyright
© 2004 Cambridge University Press

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