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Induction of Autophagy interferes the tachyzoite to bradyzoite transformation of Toxoplasma gondii

Published online by Cambridge University Press:  01 March 2016

XIANGZHI LI ,
DI CHEN ,
QIANQIAN HUA ,
YUJING WAN ,
LINA ZHENG ,
YANGYANG LIU ,
JIAXIN LIN ,
CHANGWANG PAN ,
XIN HU  and
FENG TAN
XIANGZHI LI
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
DI CHEN
Affiliation:
School of the First Clinical Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
QIANQIAN HUA
Affiliation:
Clinical Laboratory, Dongyang People's Hospital, Jinhua, Zhejiang 322100, People's Republic of China
YUJING WAN
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
LINA ZHENG
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
YANGYANG LIU
Affiliation:
School of Medical Laboratory Science and School of Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
JIAXIN LIN
Affiliation:
School of Medical Laboratory Science and School of Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
CHANGWANG PAN
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
XIN HU*
Affiliation:
School of Medical Laboratory Science and School of Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
FENG TAN*
Affiliation:
Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China
*
*Corresponding authors. School of Medical Laboratory Science and School of Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China; Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China. E-mail: gail529@163.com; tanfengsong@163.com
*Corresponding authors. School of Medical Laboratory Science and School of Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China; Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, People's Republic of China. E-mail: gail529@163.com; tanfengsong@163.com
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Summary

Autophagy process in Toxoplasma gondii plays a vital role in regulating parasite survival or death. Thus, once having an understanding of certain effects of autophagy on the transformation of tachyzoite to bradyzoite this will allow us to elucidate the function of autophagy during parasite development. Herein, we used three TgAtg proteins involved in Atg8 conjugation system, TgAtg3, TgAtg7 and TgAtg8 to evaluate the autophagy level in tachyzoite and bradyzoite of Toxoplasma in vitro based on Pru TgAtg7-HA transgenic strains. We showed that both TgAtg3 and TgAtg8 were expressed at a significantly lower level in bradyzoites than in tachyzoites. Importantly, the number of parasites containing fluorescence-labelled TgAtg8 puncta was significantly reduced in bradyzoites than in tachyzoites, suggesting that autophagy is downregulated in Toxoplasma bradyzoite in vitro. Moreover, after treatment with drugs, bradyzoite-specific gene BAG1 levels decreased significantly in rapamycin-treated bradyzoites and increased significantly in 3-MA-treated bradyzoites in comparison with control bradyzoites, indicating that Toxoplasma autophagy is involved in the transformation of tachyzoite to bradyzoite in vitro. Together, it is suggested that autophagy may serve as a potential strategy to regulate the transformation.

Keywords

Toxoplasma gondiiautophagytransformationtachyzoitebradyzoite
Type
Research Article
Information
Parasitology , Volume 143 , Issue 5 , April 2016 , pp. 639 - 645
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Copyright
Copyright © Cambridge University Press 2016 

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