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The interaction between the gelatin-binding domain of fibronectin and the attachment of Pasteuria penetrans endospores to nematode cuticle

Published online by Cambridge University Press:  28 November 2001

S. MOHAN ,
S. FOULD  and
K. G. DAVIES
S. MOHAN
Affiliation:
IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK Indian Agricultural Research Institute, New Delhi, 110012, India
S. FOULD
Affiliation:
UMR 5557 Laboratoire d'Ecologie Microbienne 43 Quai du 11 Novembre 1918, 69622 Villeubanne Cedex France
K. G. DAVIES
Affiliation:
IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK
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Abstract

Pasteuria penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kDa heparin-binding domain (HBD) and the 45 kDa gelatin-binding domain (GBD) fragments of human fibronectin (Fn) revealed a series of polypeptides of approximately 40, 45 and 55 kDa present in crude cuticle extracts of Meloidogyne javanica 2nd-stage juveniles. The results suggest that the structure of the nematode fibronectin is different to the fibronectins so far characterized. Pre-treatment of endospores of Pasteuria with either the HBD or the GBD was found to inhibit binding to the nematode cuticle. The larger GBD fragment was the most effective at blocking adhesion. Pre-treatment of the GBD fragment with gelatin prevented the GBD fragment from inhibiting endospore attachment to the nematode cuticle.

Keywords

NematodaMeloidogyne javanicabacterial adhesionextracellular matrixbiological control

Information

Type
Research Article
Information
Parasitology , Volume 123 , Issue 3 , March 2001 , pp. 271 - 276
Copyright
© 2002 Cambridge University Press

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