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Intranasal vaccination with killed Leishmania amazonensis promastigotes antigen (LaAg) associated with CAF01 adjuvant induces partial protection in BALB/c mice challenged with Leishmania (infantum) chagasi.

  • JANINE MIRANDA LEAL (a1), MARCELLE MOSQUINI (a1), LUCIANA POLACO COVRE (a1), NATALY PESCINALLI STAGMILLER (a2), RODRIGO RIBEIRO RODRIGUES (a1), DENNIS CHRISTENSEN (a3), HERBERT LEONEL DE MATOS GUEDES (a4), BARTIRA ROSSI-BERGMANN (a4) and DANIEL CLÁUDIO DE OLIVIERA GOMES (a1) (a2)...

Summary

The CAF01 adjuvant has previously been shown to be safe for human use and to be a potent adjuvant for several vaccine antigens. In the present work, we sought to optimize the Leishmania amazonensis antigens (LaAg) intranasal vaccine in an attempt to enhance the protective immune responses against Leishmania (infantum) chagasi by using the CAF01 association. LaAg/CAF01 vaccinated mice that were challenged 15 days after booster dose with L. (infantum) chagasi showed a significant reduction in their parasite burden in both the spleen and liver, which is associated with an increase in specific production of IFN-γ and nitrite, and a decrease in IL-4 production. In addition, LaAg/CAF01 intranasal delivery was able to increase lymphoproliferative immune responses after parasite antigen recall. These results suggest the feasibility of using the intranasal route for the delivery of crude antigens and of a human-compatible adjuvant against visceral leishmaniasis.

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Corresponding author

* Corresponding author. Laboratório de Imunobiologia, Núcleo de Doenças Infecciosas/Núcleo de Biotecnologia, Universidade Federal do Espirito Santo – UFES, Av. Marechal Campos, 1468 – Maruípe, Vitória – ES, Cep 29040-091, Brazil. E-mail: dgomes@ndi.ufes.br

References

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