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Karyotype variability in Trypanosoma rangeli

Published online by Cambridge University Press:  06 April 2009

J. Henriksson ,
A. Solari ,
M. Rydåker ,
O. E. Sousa  and
U. Pettersson
J. Henriksson
Affiliation:
Department of Medical Genetics, Biomedical Center, Uppsala University, Box 589, S-751 23 Uppsala, Sweden
A. Solari
Affiliation:
Departamento de Bioquimica, Division de Ciencias Medicos Norte, Universidad de Chile, Santiago, Chile
M. Rydåker
Affiliation:
Department of Medical Genetics, Biomedical Center, Uppsala University, Box 589, S-751 23 Uppsala, Sweden
O. E. Sousa
Affiliation:
Centro de Investigacióne y Diagnóstico de Enfermedades Parasitarias (CIDEP), Universidad de Panamá, Panama City, Panama
U. Pettersson
Affiliation:
Department of Medical Genetics, Biomedical Center, Uppsala University, Box 589, S-751 23 Uppsala, Sweden
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Summary

The molecular karyotypes of several different protozoan parasites show high intra-species variation, including different kinetoplastids such as Trypanosoma brucei, Trypanosoma cruzi and Leishmania ssp. In this study, the molecular karyotype of Trypanosoma rangeli was examined. To evaluate potential intra-species molecular karyotype variations, 16 different samples were studied by pulsed field gel electrophoresis (PFGE) followed by ethidium bromide staining and hybridizations with 6 different probes. The result showed that different T. rangeli populations are highly polymorphic regarding the molecular karyotype, and thus suggests that PFGE analysis can be used for classification of different T. rangeli isolates. In addition, the molecular karyotype of T. rangeli was compared to molecular karyotypes of other kinetoplastids, and was shown to be distinctly different from that of T. cruzi, but shows some similarities with the karyotype described for T. brucei. Among the probes used one was identified as highly polymorphic, and thus informative for studies of different T. rangeli populations, and another was useful for differentiation between T. rangeli and T. cruzi.

Keywords

karyotype variabilitypulsed field gel electrophoresisTrypanosoma rangeliTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 112 , Issue 4 , April 1996 , pp. 385 - 391
Copyright
Copyright © Cambridge University Press 1996

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