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Miracidia infective for snails derived from eggs laid by adult Schistosoma mansoni in vitro

Published online by Cambridge University Press:  06 April 2009

G. R. Newport  and
T. H. Weller
G. R. Newport
Affiliation:
Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts
T. H. Weller
Affiliation:
Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts
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Summary

Various biological mixtures commonly used as microbiological nutrients were added to a chemically defined medium, containing 0·2% (w/v) albumin, in order to assess their ability to support maturation of eggs deposited by adult Schistosoma mansoni in vitro. Of the supplements tested, only lactalbumin hydrolysate and casein hydrolysate stimulated egg deposition and subsequent egg development in vitro. Upon addition of foetal calf serum, further stimulation was noted only in casein hydrolysate-containing media. The addition of mouse red blood cells did not significantly improve the system. Some miracidia which hatched from eggs derived from casein hydrolysate-fortified cultures displayed normal behavioural characteristics, penetrated susceptible snails and established patent infections. These results provide an experimental system for exploring the nutritional requirements of developing miracidia, and the physiological determinants that influence miracidial penetrative capacity and subsequent ability to develop in a susceptible intermediate host.

Type
Research Article
Information
Parasitology , Volume 84 , Issue 3 , June 1982 , pp. 481 - 490
Copyright
Copyright © Cambridge University Press 1982

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