Skip to main content Accessibility help
×
Home

Morphology and Life-cycle of an Amoeba Producing Amoebiasis in Reptiles1

  • Quentin M. Geiman (a1) and Herbert L. Ratcliffe (a1)

Extract

The morphology and life cycle of an amoeba producing amoebiasis in reptiles is described. The organism is assigned to the species Entamoeba invadens Rodham 1934, because of the pathogenicity of that species.

Material for the study was derived from 30 individuals of 7 species of reptiles, either dying of spontaneous amoebiasis or experimentally infected. (Material from 3 additional species of reptiles dying of amoebiasis was not used in this study.)

With the exception of a few minor morphological differences, E. invadens was found to be strikingly similar to E. histolytica in morphology of trophic and cystic stages, and in the processes of encystation, excystation, and metacystic development.

Metacystic development was studied in stained preparations made from the intestines of snakes 1–24 hours after the inoculation by mouth of mature cysts. Excystation usually takes place in the jejunum and ileum, a single quadrinucleate amoeba hatching from each cyst. Each quadrinucleate metacystic amoeba ultimately forms 8 amoebulae as a result of one division of each cystic nucleus, cytoplasmic division, and parcelling out of the cystic (N) and daughter (n) nuclei. Nineteen of the 24 possible combinations of N and n nuclei were observed.

The culture phenomena of multiple fission without encystment is suggested, and cannibalism of cysts by the trophozoites is described.

Copyright

References

Hide All
Alexeieff, A. (1912). Sur quelques protistes parasites intestinaux d'une tortue de Ceylan (Nicoria trijuga). Zool. Anz. 40, 97.
Barret, H. P. and Smith, N. M. (1924). The cultivation of an Endamoeba from the turtle, Chelydra serpentina. Amer. J. Hyg. 4, 155.
Carini, A. & Reichenow, E. (1935). Über Amöbenmfektion in Zelleriellen. Arch. Protistenk. 84, 101.
Chatton, E. (1917). L'éclosion des kystes et les premiers stades de l'évolution de l'amibe dysentérique humaine chez le chat. Bull. Soc. Path. exot. 10, 834.
Chiang, S. F. (1925). The rat as a possible carrier of the dysentery amoeba. Proc. nat. Aced. Sci., Wash., 11, 239.
Cleveland, L. R. & Sanders, E. P. (1930). Encystation, multiple fission without encystment, excystation, metacystic development, and variation in a pure line and nine strains of Entamoeba histolytica. Arch. Protistenk. 70, 223.
Cunha, A. M. Da & Fonseca, O. Da (1917). Sobre uma nova entameba, Entamoeba serpentis, n.sp. Brazil-med. 31, 279.
Cunha, A. M. Da & Fonseca, O. Da (1918). Sobre a Entamoeba serpentis. Mem. Inst. Osw. Cruz, 10, 95.
Dobell, C. (1918). Are Entamoeba histolytica and Entamoeba ranarum the same species? An experimental inquiry. Parasitology, 10, 294.
Dobell, C. (1919). The Amoebae Diving in Man. London.
Dobell, C. (1928). Researches on the intestinal Protozoa of monkeys and man. I and II. Parasitology, 20, 357.
Hartmann, M. (1910). Ueber eine neue Darmamoebe, Entamoeba testudinis, n.sp. Mem. Inst. Osw. Cruz, 2, 3.
Hegner, R. & Chu, H. J. (1930). A comparative study of the intestinal Protozoa of wild monkeys and man. Amer. J. Hyg. 12, 62.
Hegner, R., Johnson, C. M. & Stabler, R. M. (1932). Host-parasite relations in experimental amoebiasis in monkeys in Panama. Amer. J. Hyg. 15, 394.
Knowles, R. & Das Gupta, B. M. (1930). On two intestinal Protozoa of an Indian turtle. Indian J. med. Res. 18, 97.
Lavier, G. (1928). Entamueba varan, i n.sp. amibe parasite de reptile. Ann. Parasit. hum. comp. 6, 152.
Nagahana, M. (1934). The rat as a carrier of Entamoeba histolytica. 1. The morphology and cultivation of a histolytica-like amoeba found in the wild rat (Mus norvegicus Erxl.). J. Chosen med. Ass. 24, 860.
Plimmer, H. G. (1916). Report on the deaths which occurred in the Zoological Gardens during 1915, together with a list of the blood parasites found during the year. Proc. root. Soc. Lend. p. 77.
Ratcliffe, H. L. (1934). Gastric mucin as a culture medium for intestinal Protozoa. Proc. Soc. exp. Biol., N.Y., 31, 602.
Ratcliffe, H. L. & Geiman, Q. M. (1933). Eleven cases of amoebiasis in reptiles. J. Parasit. 20, 139.
Ratcliffe, H. L. & Geiman, Q. M. (1934 a). Amebiasis in reptiles. Science, 79, 324.
Ratcliffe, H. L. & Geiman, Q. M. (1934 b). Experimental amebic infection in reptiles. J. Parasit. 20, 328.
Rodhain, J. (1934). Entamoeba invadens n.sp., parasite de serpents. C.R. Soc. Biol., Paris, 117, 1195.
Rodhain, J. & van Hoof, M. Th. (1934). Nĉte préliminaire sur le rôle pathogéne possible d'Entamoeba invadens chez les serpents. C.R. Soc. Biol. 117, 1199.
Rodhain, J. & van Hoof, M. Th. (1935). Sur le rôle pathogéne d'Entamoeha invadens. C.R. Soc. Biol. 118, 1646.
Sanders, E. P. (1931). The life-cycle of Entamoeba ranarum, Grassi (1879). Arch. Protistenk. 74, 365.
Sanders, E. P. & Cleveland, L. R. (1930). The morphology and life-cycle of Entamoeba terrapinae spec. nov. from the terrapin, Chrysemys elegans. Arch. Protistenk. 70, 267.
Stabler, R. M. (1933). On an amoeba parasitic in Zelleriella (Protozoa, Ciiata). J. Parasit. 20, 122.
Taliaferro, W. H. & Holmes, F. O. (1924). Endamoe babarreti, n.sp., from the turtle, Chetydra serpentina; a description of the amoeba from the vertebrate host and from Barret and Smith's cultures. Amer. J. Hyg. 4, 160.
Tanabe, M. (1934). The excystation and metacystic development of Entamoeba histolytica in the intestines of white rats. Keijo J. Med. 5, 238.
Wenyon, C. M. (1920). Observations on the intestinal Protozoa of three Egyptian lizards, with a note on a cell-invading fungus. Parasitology, 12, 350.
Wenyon, C. M. (1926). Protozoology. 2 vols. New York.

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed