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Morphometric study of eosinophils, mast cells, macrophages and fibrosis in the colon of chronic chagasic patients with and without megacolon

  • A. B. M. da SILVEIRA (a1), S. J. ADAD (a2), R. CORREA-OLIVEIRA (a3), J. B. FURNESS (a4) and D. D'AVILA REIS (a1)...

The mechanisms involved in the pathogenesis of chagasic megacolon are not completely characterized. Although autoimmunity may play a role in the pathogenesis of Chagas' disease, recent studies suggest a positive association of tissue parasitism, inflammation, and severity of lesions. The aim of this study was to evaluate the role of inflammatory cells and the occurrence of fibrosis in the colon of chagasic patients with and without megacolon. Samples from 26 patients were randomly selected and paraffin-embedded tissue blocks were sectioned and evaluated by histology and immunohistochemistry to analyse the occurrence and relation among eosinophils, mast cells, macrophages and fibrosis. Section analyses showed that the presence of eosinophils and mast cells in the analysed inflammatory cells has a direct correlation with fibrosis density in the chagasic megacolon. These data suggest that the megacolon's pathogenesis is based on a continuous process of cell damage. Our data propose that eosinophils, mast cells and macrophages may have a direct connection with the occurrence of fibrosis in the colon of chagasic patients. We believe that potential therapeutic agents against these cells could avoid the fibrosis process and contribute to prevent the development of chagasic megacolon.

Corresponding author
*Corresponding author: Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, Minas Gerais, Brazil, CEP: 31270-901. Fax: +55 31 3499 2771. E-mail:
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S. J. Adad , C. G. Cancado , R. M. Etchebehere , V. P. Teixeira , U. A. Gomes , E. Chapadeiro and E. R. Lopes (2001). Neuron count reevaluation in the myenteric plexus of chagasic megacolon after morphometric neuron analysis. Virchows Archiv 438, 254258.

M. G. Bachem , Z. Zhou , S. Zhou and M. Siech (2006). Role of stellate cells in pancreatic fibrogenesis associated with acute and chronic pancreatitis. Journal of Gastroenterology and Hepatology 21 (Suppl. 3), S92S96.

G. M. Cardoso , M. J. Morato , J. A. Gomes , M. O. Rocha , I. P. Bonfim , S. Williams-Blangero , J. L. VandeBerg , M. R. Reis , E. F. Magalhaes and R. Correa-Oliveira (2006). Comparative analysis of cell phenotypes in different severe clinical forms of Chagas' disease. Frontiers in Bioscience 11, 11581163.

C. E. Corbett , U. Ribeiro Jr., M. G. Prianti , A. Habr-Gama , M. Okumura and J. Gama-Rodrigues (2001). Cell-mediated immune response in megacolon from patients with chronic Chagas' disease. Diseases of the Colon and Rectum 44, 993998.

A. B. da Silveira , R. M. Arantes , A. R. Vago , E. M. Lemos , S. J. Adad , R. Correa-Oliveira and D. D'Avila Reis (2005). Comparative study of the presence of Trypanosoma cruzi kDNA, inflammation and denervation in chagasic patients with and without megaesophagus. Parasitology 131, 627634.

A. Daryani , A. Z. Hosseini and A. Dalimi (2003). Immune responses against excreted/secreted antigens of Toxoplasma gondii tachyzoites in the murine model. Veterinary Parasitology 113, 123134.

J. C. Dias , A. C. Silveira and C. J. Schofield (2002). The impact of Chagas disease control in Latin America: a review. Memórias do Instituto Oswaldo Cruz 97, 603612.

G. A. DosReis , C. G. Freire-de-Lima , M. P. Nunes and M. F. Lopes (2005). The importance of aberrant T-cell responses in Chagas disease. Trends in Parasitology 21, 237243.

M. Hashimoto , A. Nitta , H. Fukumitsu , H. Nomoto , L. Shen and S. Furukawa (2005). Involvement of glial cell line-derived neurotrophic factor in activation processes of rodent macrophages. Journal of Neuroscience Research 79, 476487.

D. L. Hirschberg , E. Yoles , M. Belkin and M. Schwartz (1994). Inflammation after axonal injury has conflicting consequences for recovery of function: rescue of spared axons is impaired but regeneration is supported. Journal of Neuroimmunology 50, 916.

H. H. Kariyawasam and D. S. Robinson (2006). The eosinophil: the cell and its weapons, the cytokines, its locations. Seminars in Respiratory and Critical Care Medicine 27, 117127.

R. Kiefer , B. C. Kieseier , G. Stoll and H. P. Hartung (2001). The role of macrophages in immune-mediated damage to the peripheral nervous system. Progress in Neurobiology 64, 109127.

T. Kobayashi , Y. Ohta , K. Inui , J. Yoshino and S. Nakazawa (2002). Protective effect of omeprazole against acute gastric mucosal lesions induced by compound 48/80, a mast cell degranulator, in rats. Pharmacological Research 46, 7584.

F. Koberle (1968). Chagas' disease and Chagas' syndromes: the pathology of American trypanosomiasis. Advances in Parasitology 6, 63116.

A. Lakatos and R. J. Franklin (2002). Transplant mediated repair of the central nervous system: an imminent solution? Current Opinion in Neurology 15, 701705.

E. M. Lemos , D. Reis , S. J. Adad , G. C. Silva , E. Crema and R. Correa-Oliveira (1998). Decreased CD4(+) circulating T lymphocytes in patients with gastrointestinal chagas disease. Clinical Immunology and Immunopathology 88, 150155.

A. M. Levy , K. Yamazaki , V. P. Van Keulen , L. J. Burgart , W. J. Sandborn , S. F. Phillips , G. M. Kephart , G. J. Gleich and K. M. Leiferman (2001). Increased eosinophil infiltration and degranulation in colonic tissue from patients with collagenous colitis. The American Journal of Gastroenterology 96, 15221528.

R. C. Melo and C. R. Machado (2001). Trypanosoma cruzi: peripheral blood monocytes and heart macrophages in the resistance to acute experimental infection in rats. Experimental Parasitology 97, 1523.

L. M. Mendell , K. M. Albers and B. M. Davis (1999). Neurotrophins, nociceptors, and pain. Microscopy Research and Technique 45, 252261.

A. Micera , I. Puxeddu , L. Aloe and F. Levi-Schaffer (2003). New insights on the involvement of Nerve Growth Factor in allergic inflammation and fibrosis. Cytokine and Growth Factor Reviews 14, 369374.

Y. Motomura , W. I. Khan , R. T. El-Sharkawy , M. Verma-Gandhu , E. F. Verdu , J. Gauldie and S. M. Collins (2006). Induction of a fibrogenic response in mouse colon by overexpression of monocyte chemoattractant protein 1. Gut 55, 662670.

O. Noga , C. Englmann , G. Hanf , A. Grutzkau , J. Seybold and G. Kunkel (2003). The production, storage and release of the neurotrophins nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3 by human peripheral eosinophils in allergics and non-allergics. Clinical and Experimental Allergy 33, 649654.

O. Noga , G. Hanf , D. Gorges , Q. Thai Dinh , D. A. Groneberg , N. Suttorp and G. Kunkel (2005). Regulation of NGF and BDNF by dexamethasone and theophylline in human peripheral eosinophils in allergics and non-allergics. Regulatory Peptides 132, 7479.

M. J. Nowicki , C. Chinchilla , L. Corado , L. Matsuoka , R. Selby , F. Steurer , T. Mone , R. Mendez and S. Aswad (2006). Prevalence of antibodies to Trypanosoma cruzi among solid organ donors in Southern California: a population at risk. Transplantation 81, 477479.

M. C. Pinheiro , P. S. Beraldo , L. F. Junqueira Junior , E. R. Lopes and E. Chapadeiro (1992). [A quantitative analysis of the mastocytes and eosinophilic granulocytes in the myocardium of Wistar rats chronically infected by Trypanosoma cruzi. A contribution to the knowledge of myocardial fibrosis]. Revista da Sociedade Brasileira de Medicina Tropical 25, 4550.

S. W. Pinheiro , A. M. Rua , R. M. Etchebehere , C. G. Cancado , J. E. Chica , E. R. Lopes and S. J. Adad (2003). Morphometric study of the fibrosis and mast cell count in the circular colon musculature of chronic Chagas patients with and without megacolon. Revista da Sociedade Brasileira de Medicina Tropical 36, 461466.

C. Sarkar , R. Lakhtakia , S. S. Gill , M. C. Sharma , A. K. Mahapatra and V. S. Mehta (2002). Chronic subdural haematoma and the enigmatic eosinophil. Acta Neurochirurgica 144, 983988; discussion 988.

U. Slawinska , H. Majczynski and R. Djavadian (2000). Recovery of hindlimb motor functions after spinal cord transection is enhanced by grafts of the embryonic raphe nuclei. Experimental Brain Research 132, 2738.

L. E. Smythies , M. Sellers , R. H. Clements , M. Mosteller-Barnum , G. Meng , W. H. Benjamin , J. M. Orenstein and P. D. Smith (2005). Human intestinal macrophages display profound inflammatory anergy despite avid phagocytic and bacteriocidal activity. The Journal of Clinical Investigation 115, 6675.

A. Stepanyants and D. B. Chklovskii (2005). Neurogeometry and potential synaptic connectivity. Trends in Neuroscience 28, 387394.

A. Straumann and H. U. Simon (2004). The physiological and pathophysiological roles of eosinophils in the gastrointestinal tract. Allergy 59, 1525.

P. Vergara , Y. Saavedra and C. Juanola (2002). Neuroendocrine control of intestinal mucosal mast cells under physiological conditions. Neurogastroenterology and Motility 14, 3542.

X. Xu , A. Rivkind , A. Pikarsky , O. Pappo , S. C. Bischoff and F. Levi-Schaffer (2004). Mast cells and eosinophils have a potential profibrogenic role in Crohn disease. Scandinavian Journal of Gastroenterology 39, 440447.

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