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Natural distribution and co-infection patterns of microsporidia parasites in the Daphnia longispina complex

Published online by Cambridge University Press:  12 March 2012

S. WEIGL ,
H. KÖRNER ,
A. PETRUSEK ,
J. SEDA  and
J. WOLINSKA
S. WEIGL
Affiliation:
Ludwig-Maximilians-Universität München, Department Biologie II, Großhaderner Strasse 2, 82152 Planegg-Martinsried, Germany
H. KÖRNER
Affiliation:
Department of Neuroimmunology, Institute for Multiple Sclerosis Research, University Medical Center Göttingen and The Hertie Foundation, Göttingen, Germany
A. PETRUSEK
Affiliation:
Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 12844 Prague, Czech Republic
J. SEDA
Affiliation:
Biological Centre AS CR, Institute of Hydrobiology, Na Sádkách 7, 37005 České Budějovice, Czech Republic
J. WOLINSKA*
Affiliation:
Ludwig-Maximilians-Universität München, Department Biologie II, Großhaderner Strasse 2, 82152 Planegg-Martinsried, Germany
*
*Corresponding author: Ludwig-Maximilians-Universität München, Department Biologie II, Großhaderner Strasse 2, 82152 Planegg-Martinsried, Germany. Tel: +49 (0) 89 2180 74 201. Fax: +49 (0) 89 2180 204. E-mail: wolinska@bio.lmu.de
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Summary

Microsporidia are intracellular parasites, frequently infecting the planktonic crustacean Daphnia. Questioning the ability to detect and identify microsporidia with conventional microscopic techniques, we applied molecular methods in order to investigate the distribution and co-infection patterns of this parasite among 8 communities of the Daphnia longispina hybrid complex. Eight microsporidian taxa were detected, including 3 that previously had not been characterized genetically. Microsporidian communities from nearby lakes were found to be more similar to each other, apparently due to short distance dispersal via secondary hosts. Moreover, we detected seasonal (but not interannual) changes in microsporidian community structure. With some microsporidia being host-specific, these changes might have resulted from seasonal changes in host taxon and clonal composition. The 2 dominant and closely related parasite species were found mainly in single infections, whereas another pair of related microsporidians was found predominantly in co-infections; suggesting species-level differences in the ability to colonize infected hosts. By applying molecular methods, we were not only able to unambiguously identify parasite taxa but also to reveal multiple infections that otherwise would have remained undetected. Given the increased level of accuracy and sensitivity, we highly recommend molecular approaches in future parasite surveys of Daphnia infections.

Keywords

co-infectionDaphniamicrosporidiamultiple infectionsparasite distribution
Type
Research Article
Information
Parasitology , Volume 139 , Issue 7 , June 2012 , pp. 870 - 880
Copyright
Copyright © Cambridge University Press 2012

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