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Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group

Published online by Cambridge University Press:  06 April 2009

J. A. T. Morgan  and
D. Blair
J. A. T. Morgan
Affiliation:
Zoology Department, James Cook University, Townsville, Queensland, Australia4811
D. Blair
Affiliation:
Zoology Department, James Cook University, Townsville, Queensland, Australia4811
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Summary

The taxonomic history of members of the 37-collar-spine group within the genus Echinostoma has been very confused. We obtained DNA sequence data from the nuclear rDNA ITS1, 5·8S and ITS2 of 7 nominal species belonging to this group, Echinostoma trivolvis (Cort, 1914), E. revolution (Frölich, 1802), E. caproni Richard, 1964, E. liei Jeyarasasingam et al. 1972, E. paraensei Lie & Basch, 1967, two African isolates, E. sp.I and E. sp.II, and of one 28-collar-spined echinostome, E. hortense (Asada, 1926). Five of the eight species were clearly distinguishable using ITS data. Sequences from the remaining three taxa, E. caproni, E. sp.II and E. liei were identical to one another and the group containing these taxa was distant from other 37-collar-spine species on a phylogenetic tree. E. trivolvis and E. paraensei form a second, but less distinct group within the 37-collar-spine group. The resolution obtained using DNA sequencing will assist in the current reclassification of the group. It also provides a model for future work on sibling species.

Keywords

internal transcribed spacerEchinostomanucleotide sequencephylogenyTrematodaribosomal DNA
Type
Research Article
Information
Parasitology , Volume 111 , Issue 5 , December 1995 , pp. 609 - 615
Copyright
Copyright © Cambridge University Press 1995

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References

REFERENCES

Adlard, R. D., Barker, S. C., Blair, D. & Cribb, T. H. (1993). Comparison of the second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea). International Journal for Parasitology 23, 423–5.CrossRefGoogle ScholarPubMed
Asada, J. (1926). Ueber eine neue Species von Echinochasmus und ihren Entwicklungsgang. [Abstract]. Transactions of the Japanese Pathological Society 16, 113.Google Scholar
JrBailey, R. S. & Fried, B. (1977). Thin layer chromatographic analyses of amino acids in Echinostoma revoltitum (Trematoda) adults. International Journal for Parasitology 7, 497–9.CrossRefGoogle ScholarPubMed
Ball, J. K. & Desselberger, U. (1992). Incorporation of single-stranded DXA binding protein early in polymerase chain reaction product sequencing reactions prevents enzyme pausing. Analytical Biochemistry 207, 349–51.CrossRefGoogle Scholar
Barseine, J. & Kiseliene, V. (1991) Karyological studies of Trematodes within the genus Echinostoma. Acta Parasitologica Polonica 36, 25–30.Google Scholar
Bowles, J., Blair, D. & McManus, D. P. (1995). A molecular phylogeny of the human schistosomes. Molecular Phylogenetics and Evolution 4, 103–9.CrossRefGoogle ScholarPubMed
Cabot, E. L. & Beckenbach, A. T. (1989). Simultaneous editing of multiple nucleic acid and protein sequences with ESEE. Computer Applications in the Biosciences 5, 233–4.Google ScholarPubMed
Christensen, N. ø., Fried, B. & Kanev, I. (1990). Taxonomy of 37-collar spined Echinostoma (Trematoda: Echinostomatidae) in studies on the population regulation in experimental rodent hosts. Angewandte Parasitologie 31, 127–30.Google ScholarPubMed
Cort, W. W. (1914). Larval trematodes from North American fresh water snails. Preliminary report. Journal of Parasitology 1, 65–84.CrossRefGoogle Scholar
Després, L., Imbert-Establet, D., Combes, C. & Bonhomme, F. (1992). Molecular evidence linking hominid evolution to recent radiations of schistosomes. Molecular Phylogenetics and Evolution 1, 295–304.CrossRefGoogle ScholarPubMed
Dover, G. A. (1982). Molecular drive: A cohesive mode of species evolution. Nature, London 299, 111–17.CrossRefGoogle ScholarPubMed
Fried, B. & Boddorff, J. M. (1978). Neutral lipids in Echinostoma revolutum (Trematoda) adults. Journal of Parasitology 64, 174–5.CrossRefGoogle ScholarPubMed
Fried, B. & Morrone, L. J. (1970). Histochemical lipid studies on Echinostoma revolutum. Proceedings of the Helminthological Society of Washington 37, 122–3.Google Scholar
Frölich, J. A. (1802). BeytrÄge zur Naturgeschichte der Eingeweidewürmer. Naturforscher, Halle 29, 5–96.Google Scholar
Hillis, D. M. & Dixon, M. T. (1991). Ribosomal DNA: Molecular evolution and phylogenetic inference. Quarterly Review of Biology 66, 411–46.CrossRefGoogle ScholarPubMed
Huffman, J. E. & Fried, B. (1990). Echinostoma and echinostomiasis. Advances in Parasitology 29, 215–69.CrossRefGoogle ScholarPubMed
Jeyarasasingam, U., Heyneman, D., Hok-Kan, Lim & Mansour, N. (1972). Life-cycle of a new echinostome from Egypt, Echinostoma liei sp. nov. (Trematoda: Echinostomatidae). Parasitology 65, 203–22.CrossRefGoogle Scholar
Kane, R. A. & Rollinson, D. (1994). Repetitive sequences in the ribosomal DNA internal spacer of Schistosoma haematobium, Schistosoma intercalatum and Schistosoma mattheei. Molecular and Biochemical Parasitology 63, 153–6.CrossRefGoogle ScholarPubMed
Kanev, I. (1985). On the morphology, biology, ecology and taxonomy of E. revolutum group (Trematoda: Echinostomatidae: Echinostoma) Ph.D. dissertation, University of Sofia, Bulgaria. (English abstract.)Google Scholar
Kanev, I. (1994) Life-cycle, delimination and redescription of Echinostoma revolutum (Froelich, 1802) (Trematoda: Echinostomatidae). Systematic Parasitology 28, 125–44.CrossRefGoogle Scholar
Kristensen, A. R. & Fried, B. (1991). A comparison of Echinostoma caproni and Echinostoma trivolvis (Trematoda: Echinostomatidae) adults using isoelectric-focusing. Journal of Parasitology 77, 496–8.CrossRefGoogle Scholar
Kwon, O.-Y. & Ishikawa, H. (1992). Nucleotide sequence and presumed secondary structure of the internal transcribed spacers of rDXA of the pea aphid Acyrthosiphon pisum. Comparative Biochemistry and Physiology 103B, 651–5.Google Scholar
Lee, S. B. & Taylor, J. W. (1992). Phylogeny of five fungus like Protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA. Molecular Biology and Evolution 9, 636–53.Google ScholarPubMed
Lie, K. J. & Basch, P. F. (1967). The life history of Echinostoma paraensei sp.n. (Trematoda: Echinostomatidae). Journal of Parasitology 53, 1192–9.CrossRefGoogle ScholarPubMed
Luton, K., Walker, D. & Blair, D. (1992). Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Trematoda: Digenea). Molecular and Biochemical Parasitology 56, 323–8.CrossRefGoogle ScholarPubMed
Porter, C. H. & Collins, F. H. (1991). Species-diagnostic differences in a ribosomal DNA internal transcribed spacer from the sibling species Anopheles freeborni and Anopheles hermsi (Diptera: Culicidae). American Journal of Tropical Medicine and Hygiene 45, 271–9.CrossRefGoogle Scholar
Richard, J. (1964). Trematodes d'oiseaux de Madagascar (Note III). Espèces de la famille Echinostomatidae Poche 1926. Annales de Parasitologie Humaine et Comparée. 39, 607–20.CrossRefGoogle Scholar
Richard, J. & Voltz, A. (1987). Preliminary data on the chromosomes of Echinostoma caproni Richard 1964 (Trematoda: Echinostomatidae). Systematic Parasitology 9, 169–72.CrossRefGoogle Scholar
Ross, G. C., Fried, B. & Southgate, V. R. (1989). Echinostoma revolutum and E. liei: Observations on enzymes and pigments. Journal of Natural History 23, 977–81.CrossRefGoogle Scholar
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. Second Edn.Cold Spring Harbour: Cold Spring Harbour Laboratory Press, USA.Google Scholar
Sleckman, B. P. & Fried, B. (1984). Glycogen and protein content in adult Echinostoma revolutum (Trematoda). Proceedings of the Helminthological Society of Washington 51, 355–6.Google Scholar
Swofford, D. L. (1993). PAUP: Phylogenetic analysis using parsimony version 3.3_legacy Computer program distributed by the Illinois Natural History Survey, Campaign, Illinois.Google Scholar
Peer, Y. Van De & Wachter, R. De (1993). TREECON: A Software package for the construction and drawing of evolutionary trees. CABIOS 9, 177–82.Google ScholarPubMed
Voltz, A., Richard, J., Pesson, B. & Jourdane, J. (1988). Isoenzyme analysis of Echinostoma liei: Comparison and hybridization with other African species. Experimental Parasitology 66, 13–17.CrossRefGoogle ScholarPubMed