Skip to main content Accessibility help
×
Home

Polymorphism in Plasmodium vivax MSA1 gene – the result of intragenic recombinations?

  • Q. Cheng (a1), A. Stowers (a1), T.-Y. Huang (a1), D. Bustos (a2), Y.-M. Huang (a3), C. Rzepczyk (a1) and A. Saul (a1)...

Summary

The diversity in a 925 bp portion of the Plasmodium vivax MSA1 gene in isolates from the Philippines, China, the Solomon Islands and Papua New Guinea was investigated. A total of 74 base pair changes was found in the amplified fragment from 18 isolates. Most of these changes were single or double base pair substitutions. In several regions, these point changes were tightly linked with one set always present or always absent in the different isolates. Seven such blocks were identified. These blocks were present in different combinations in the different isolates indicating that extensive intragenic recombination has occurred.

Copyright

References

Hide All
Ayala, F. J. & Fitch, W. M. (1992). Phylogeny of Plasmodium falciparum. Parasitology Today 8, 74–5.
Conway, D. J. & McBride, J. S. (1991). Population genetics of Plasmodium falciparum within a malaria hyperendemic area. Parasitology 103, 716.
Conway, D. J., Greenwood, B. M. & McBride, J. S. (1992). Longitudinal study of Plasmodium falciparum polymorphic antigens in a malaria-endemic population. Infection and Immunity 60, 1122–7.
Del Portillo, H. A., Longacre, S., Khouri, E. & David, p. H. (1991). Primary structure of the merozoite surface antigen 1 of Plasmodium vivax reveals sequences conserved between different Plasmodium species. Proceedings of the National Academy of Sciences, USA 88, 4030–4.
Gibson, H. L., Tucker, J. E., Kaslow, D. C., Krettli, A. U., Collins, W. E., Kiefer, M. C., Bathurst, I. C. & Barr, P. J. (1992). Structure and expression of the gene for P.v200, a major blood-stage surface antigen of Plasmodium vivax. Molecular and Biochemical Parasitology 50, 325–34.
Holder, A. A. & Freeman, R. R. (1982). Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody. Journal of Experimental Medicine 156, 1528–38.
Kemp, D. J., Cowman, A. F. & Walliker, D. (1990). Genetic diversity in Plasmodium falciparum. Advances in Parasitology 29, 75149.
Limpaiboon, T., Shirley, M. W., Kemp, D. J. & Saul, A. (1991). 7H8/6, a multicopy DNA probe for distinguishing isolates of Plasmodium falciparum. Molecular and Biochemical Parasitology 47, 197206.
Lockyer, M. J., Marsh, K. & Newbold, C. I. (1989). Wild isolates of Plasmodium falciparum show extensive polymorphism in T cell epitopes of the circumsporozoite protein. Molecular and Biochemical Parasitology 37, 275–80.
McCutchan, T. F., Lal, A. A., Rosario, V. D. & Waters, A. P. (1992). Two types of sequence polymorphism in the circumsporozoite gene of Plasmodium falciparum. Molecular and Biochemical Parasitology 50, 3746.
Marshall, V. M., Coppel, R. L., Anders, R. F. & Kemp, D. J. (1992). Two novel alleles within subfamilies of the merozoite surface antigen 2 (MSA-2) of Plasmodium falciparum. Molecular and Biochemical Parasitology 50, 181–4.
Peterson, M. G., Coppel, R. L., McIntyre, P., Langford, C. J., Woodrow, G., Brown, G. V., Anders, R. F. & Kemp, D. J. (1988). Variation in the precursor to the major merozoite surface antigens of Plasmodium falciparum. Molecular and Biochemical Parasitology 27, 291302.
Tanabe, K., Mackay, M., Goman, M. & Scaife, J. G. (1987). Allelic dimorphism in a surface antigen gene of the malaria parasite Plasmodium falciparum. Journal of Molecular Biology 196, 273–87.
Tibayrenc, M., Kjellberg, F. & Ayala, J. F. (1990). A clonal theory of parasitic protozoa: the population structures of Entamoeba, Giardia, Leishmania, Naegleria, Plasmodium, Trichomonas, and Trypanosoma and their medical and taxonomical consequences. Proceedings of the National Academy of Sciences, USA 87, 2414–18.
Walker-Jonah, A., Dolan, S. A., Gwadz, R. W., Panton, L. j. & Wellems, T. E. (1992). An RFLP map of the Plasmodium falciparum genome, recombination rates and favored linkage groups in a genetic cross. Molecular and Biochemical Parasitology 51, 313–20.
Walliker, D. (1989). Implications of genetic exchange in the study of protozoan infections. Parasitology 99, S48S58.
Waters, A. P. & McCutchan, T. F. (1992). Plasmodium falciparum: birds to humans. Parasitology Today 8, 91–2.

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed