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Scanning electron microscopy of biopsy specimens removed by a colonoscope from the abomasum of sheep infected with Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

C. D. Nicholls ,
D. L. Lee  and
M. J. Sharpe
C. D. Nicholls
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
D. L. Lee
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
M. J. Sharpe
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
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Extract

The abomasum of living sheep infected with 50000 larvae of Haemonchus contortus was examined before and during infection, by means of fibre optic endoscopy. Biopsy specimens were removed from the abomasum of the living sheep and were examined by means of scanning electron microscopy. Changes were noted in the surface structure of the abomasum 2 days after infection and larvae were seen on, and burrowing into, the mucosa. These changes became more pronounced as the infection proceeded, especially after day 10 of the infection when the adult worms had appeared. The behaviour of adult nematodes was observed within the abomasum and on occasions they were seen to move from surrounding areas into an area of haemorrhage, caused by removal of a biopsy specimen. Individual nematodes were removed from the abomasum by means of the biopsy forceps. Fibre optic endoscopy was shown to be a useful tool in the study of parasitic nematodes in vivo.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 357 - 363
Copyright
Copyright © Cambridge University Press 1985

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References

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