Hostname: page-component-76fb5796d-22dnz Total loading time: 0 Render date: 2024-04-29T07:55:55.229Z Has data issue: false hasContentIssue false
  • English
  • Français

Schistosoma mansoni: effect of maintenance in vitro on the physiology and biochemistry of adult worms

Published online by Cambridge University Press:  06 April 2009

J. G. Mercer  and
L. H. Chappell
J. G. Mercer
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, Scotland AB9 2TN
L. H. Chappell
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, Scotland AB9 2TN
Get access Rights & Permissions [Opens in a new window]

Extract

In Medium 199 supplemented with calf serum (+ 10%), adult Schistosoma mansoni produced a mean total of 400 eggs/worm pair during maintenance for 10 days in vitro. During the period of egg deposition in vitro, the dry weight of worm pairs decreased by 35%. Biomass decreases were proportionally greater in female worms than in males. Declines in the dry weight of female worms were mainly due to decreases in protein content, while biomass decreases in males resulted from losses of both protein and glycogen. Glucose was depleted from the maintenance medium at a rate of approximately 200 μg/worm pair/day during each of the first 3 days in vitro, while concentrations of glucose in the medium of above 1 g/litre did not affect the degree of glycogen depletion observed in adult S. mansoni during culture for 24 h. The contribution of the loss of schistosome gut contents in vitro to the observed changes is discussed. The work described represents part of an attempt at logical design of in vitro culture.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 339 - 349
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Basch, P. F. & Humbert, R. (1981). Cultivation of Schistosoma mansoni in vitro. III. Implantation of cultured worms into mouse mesenteric veins. Journal of Parasitology 67, 191–5.CrossRefGoogle ScholarPubMed
Bueding, E. (1950). Carbohydrate metabolism of Schistosoma mansoni. Journal of General Physiology 33, 475–95.CrossRefGoogle ScholarPubMed
Bueding, E. & Fisher, J. (1970). Biochemical effects of Niridazole on Schistosoma mansoni. Molecular Pharmacology 6, 532–9.Google ScholarPubMed
Bueding, E. & Koletsky, S. (1950). Content and distribution of glycogen in Schistosoma mansoni. Proceedings of the Society for Experimental Biology and Medicine 73, 594–6.CrossRefGoogle ScholarPubMed
Chappell, L. H. & Walker, E. (1982). Schistosoma mansoni: incorporation and metabolism of protein amino acids in vitro. Comparative Biochemistry and Physiology 73B, 701–7.Google ScholarPubMed
Cornford, E. M., Diep, C. P. & Rowley, G. A. (1983). Schistosoma mansoni, S. japonicum, S. haematobium: glycogen content and glucose uptake in parasites from fasted and control hosts. Experimental Parasitology 56, 397–408.CrossRefGoogle ScholarPubMed
Cornford, E. M. & Huot, M. E. (1981). Glucose transfer from male to female schistosomes. Science 213, 1269–71.CrossRefGoogle Scholar
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–6.CrossRefGoogle Scholar
Floyd, R. D. & Nollen, P. M. (1977). Effects of stressful conditions on the development and movement of reproductive cells in Schistosoma mansoni. Journal of Parasitology 63, 87–90.CrossRefGoogle ScholarPubMed
Fried, B. (1978). In Methods of Cultivating Parasites in vitro (ed. Taylor, A. E. R. and Baker, J. R.). London: Academic Press.Google Scholar
Fu, H. M., Chow, K. & Chiu, J. K. (1976). in vitro cultivation of Schistosoma japonicum. International Journal of Zoonoses 3, 105–113.Google ScholarPubMed
Holden, B. L. (1967). Histochemical study of glycogen in Schistosoma mansoni. M.Sc. thesis, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland.Google Scholar
Kloetzel, K. (1967). Egg and pigment production in Schistosoma mansoni infections of the white mouse. American Journal of Tropical Medicine and Hygiene 16, 293–9.CrossRefGoogle ScholarPubMed
Lancastre, F. & Golvan, Y. (1973). L'utilisation des cultures in vitro dans l'étude de Schistosoma mansoni. I. le maintien en survie des adultes. Annales de Parasitologie 48, 307–13.Google Scholar
Lee, H. G. & Michaels, R. M. (1968). in vitro and in vivo effects of selected metabolic inhibitors and chemotherapeutic agents and egg development of Schistosoma mansoni. Experimental Parasitology 22, 256–63.CrossRefGoogle ScholarPubMed
Lennox, R. W. & Schiller, E. L. (1972). Changes in dry weight and glycogen content as criteria for measuring the postcercarial growth and development of Schistosoma mansoni. Journal of Parasitology 58, 489–94.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
Mercer, J. G. (1984). Schistosoma mansoni: biological and biochemical studies on adult worms in vitro. Ph.D. thesis, University of Aberdeen, Aberdeen, Scotland.Google Scholar
Michaels, R. M. & Prata, A. (1968). Evolution and characteristics of Schistosoma mansoni eggs laid in vitro. Journal of Parasitology 54, 921–30.CrossRefGoogle ScholarPubMed
Newport, G. R. & Weller, T. H. (1982 a). Deposition and maturation of eggs of Schistosoma mansoni in vitro: importance of fatty acids in serum-free media. American Journal of Tropical Medicine and Hygiene 31, 349–57.CrossRefGoogle ScholarPubMed
Newport, G. R. & Weller, T. H. (1982 b). Miracidia infective for snails derived from eggs laid by adult Schistosoma mansoni in vitro. Parasitology 84, 481–90.CrossRefGoogle ScholarPubMed
Robinson, D. L. H. (1956). A routine method for the maintenance of Schistosoma mansoni in vitro. Journal of Helminthology 29, 193–202.CrossRefGoogle Scholar
Robinson, D. L. H. (1960). Egg laying in Schistosoma mansoni in vitro. Annals of Tropical Medicine and Parasitology 54, 112–17.CrossRefGoogle ScholarPubMed
Schiller, E. L., Bueding, E., Turner, V. M. & Fisher, J. (1975). Aerobic and anaerobic carbohydrate metabolism and egg production of Schistosoma mansoni in vitro. Journal of Parasitology 61, 385–9.CrossRefGoogle ScholarPubMed
Senft, A. W. & Senft, D. G. (1962). A chemically defined medium for maintenance of Schistosoma mansoni. Journal of Parasitology 48, 551–4.CrossRefGoogle ScholarPubMed
Shaw, J. R. & Erasmus, D. A. (1977). Schistosoma mansoni: differential cell death associated with in vitro culture and treatment with Astiban (Roche). Parasitology 75, 101–9.CrossRefGoogle ScholarPubMed
Smith, T. M. & Brown, J. N. (1970). Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities in axenically maintained adult Schistosoma mansoni. Comparative Biochemistry and Physiology 32, 573–5.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Taylor, A. E. R. & Baker, J. R. (1968). The Cultivation of Parasites in vitro. Oxford: Blackwell Scientific Publications.Google Scholar
Walker, E. & Chappell, L. H. (1980). Schistosoma mansoni: comparison of the effects of cycloheximide and emetine on protein synthesis in adult worms. Comparative Biochemistry and Physiology 67C, 129–34.Google Scholar
Weinmann, C. J. & Hunter, G. W. (1961). Studies on schistosomiasis. XVI. The effect of immune serum upon egg production by Schistosoma mansoni in mice. Experimental Parasitology 11, 56–62.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1979). Synthesis of macromolecules by the epithelial surfaces of Schistosoma mansoni: an autoradiographic study. Parasitology 78, 295–310.CrossRefGoogle ScholarPubMed
Zussman, R. A., Bauman, P. M. & Petruska, J. C. (1970). The role of ingested haemoglobin in the nutrition of Schistosoma mansoni. Journal of Parasitology 56, 75–9.CrossRefGoogle ScholarPubMed