Hostname: page-component-8448b6f56d-m8qmq Total loading time: 0 Render date: 2024-04-24T00:01:29.825Z Has data issue: false hasContentIssue false
  • English
  • Français

Transcriptional regulation of Schistosoma mansoni calreticulin: possible role of AP-1

Published online by Cambridge University Press:  06 April 2009

J. Khalife ,
C. Godin  and
A. Capron
J. Khalife
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, INSERM U167, Institut Pasteur, I Rue de Professeur Calmette, B.P. 245, 59019-Lille, France
C. Godin
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, INSERM U167, Institut Pasteur, I Rue de Professeur Calmette, B.P. 245, 59019-Lille, France
A. Capron
Affiliation:
Centre d' Immunologie et de Biologie Parasitaire, INSERM U167, Institut Pasteur, I Rue de Professeur Calmette, B.P. 245, 59019-Lille, France
Get access Rights & Permissions [Opens in a new window]

Summary

Little is known about the regulation and control of Schistosoma mansoni gene expression. In order to study such mechanisms a gene reporter expression vector construction, under the control of a promoter region derived from the S. mansoni calreticulin gene was used to transfect the human Jurkat T cell line. The promoter region contains potential TATA and CAAT boxes as well as an AP-1 core element. We show here that transcriptional factors of eucaryotic cells may induce a gene reporter activity under the control of a S. mansoni promoter region. Treatment of stably transfected cells with N-acetyl cysteine (NAC), a well-characterized antioxidant which counteracts the effects of reactive oxygen intermediates, enhanced the AP-1 dependent transactivation. This effect was abolished when the SmCaR promoter region was deleted in the AP-1 site. Electrophoretic Mobility Shift Assays showed that the AP-1 sequence of S. mansoni bound to both S. mansoni extracts and in nuclear extracts from Jurkat cells, thus explaining possible activation of AP-1 by NAC. Finally treatment of S. mansoni schistosomula and adult worms with NAC induced an increased synthesis of calreticulin protein suggesting a possible role of redox mechanisms in the regulation of a calreticulin gene transcription process in S. mansoni.

Keywords

Schistosoma mansonicalreticulinAP-1N-acetyl cysteine
Type
Research Article
Information
Parasitology , Volume 111 , Issue 4 , November 1995 , pp. 469 - 475
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abate, C., Patel, L., Rauscher, F. J. I. & Curran, T. (1990). Redox regulation of Fos and Jun DNA-binding activity in vitro. Science 249, 1157–61.CrossRefGoogle ScholarPubMed
Angel, P. & Karin, M. (1991). The role of Jun, Fos and the AP-1 complex in cell proliferation and transformation. Biochimica et Biophysica Acta 1072, 129–57.Google ScholarPubMed
Aronstein, W. S. & Strand, M. (1984). Gender-specific and pair-dependent glycoprotein antigens of Schistosoma mansoni. Experimental Parasitology 70, 545–57.CrossRefGoogle ScholarPubMed
Atkinson, B. G. & Atkinson, K. H. (1982). Schistosoma mansoni: one- and two-dimensional electrophoresis of proteins synthesized in vitro by males, females and juveniles. Experimental Parasitology 53, 26–38.CrossRefGoogle ScholarPubMed
Chen, L. L., Rekosh, D. M. & Loverde, P. T. (1992). Schistosoma mansoni P48 eggshell protein gene characterization, developmentally regulated expression and comparison to the p14 eggshell protein gene. Molecular and Biochemical Parasitology 52, 39–52.CrossRefGoogle Scholar
Dent, S. L. & Latchman, D. S. (1993). The DNA mobility shift assay. In Transcription Factors: A Practical Approach (ed. Latchman, D. S.), pp. 1–26. Oxford: Oxford University Press.Google Scholar
Devary, Y., Gottlieb, R. A., Laus, L. F. & Karin, M. (1991). Rapid and preferential activation of the c-jun gene during the mammalian UV response. Molecular and Cellular Biology 11, 2804–11.Google ScholarPubMed
Dewet, J. R., Wood, K. V., Dehica, M., Helinski, D. R. & Subramani, S. (1987). Firefly luciferase gene/structure and expression in mammalian cells. Molecular and Cellular Biology 7, 725–37.Google Scholar
Faisst, S. & Meyer, S. (1992). Compilation of vertebrate-encoded transcription factors Nucleic Acids Research 20, 3–26.Google Scholar
Khalife, J., Liu, J. L., Pierce, R., Porchet, E., Godin, C. & Capron, A. (1994). Characterization and localization of Schistosoma mansoni calreticulin Sm58. Parasitology 108, 527–32.CrossRefGoogle ScholarPubMed
Khalife, J., Pierce, R. J., Godin, C. & Capron, A. (1933 a). Cloning and sequencing of the gene encoding Schistosoma mansoni calreticulin. Molecular and Biochemical Parasitology 62, 313–16.CrossRefGoogle Scholar
Khalife, J., Trottein, F., Schacht, A. M., Godin, C., Pierce, R. J. & Capron, A. (1933 b). Cloning of the gene encoding a Schistosoma mansoni antigen homologous to human Ro/SS-A autoantigen. Molecular and Biochemical Parasitology 57, 193–202.CrossRefGoogle Scholar
Levy-Holtzman, R. & Schechter, I. (1994). SchistOSOme extracts with heat shock factor activity revealed by the gel shift assay. Parasitology 108, 35–42.CrossRefGoogle ScholarPubMed
Michalak, M., Milner, R. E., Burns, K. & Opas, M. (1992). Calreticulin. The Biochemical Journal 285, 681–92.CrossRefGoogle ScholarPubMed
Ohtani, K., Nakamura, M., Saito, S., Nagata, K., Sugamura, K. & Hinuma, Y. (1989). Electroporation: application to human lymphoid cell lines for stable introduction of a transactivator gene of human T-cell leukemia virus type 1. Nucleic Acids Research 17, 1589–159.CrossRefGoogle Scholar
Ramalho-Pinto, G., Gazinelli, G., Howells-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni defined system for stepwise transformation of cercariae to schistosomula. Journal of Experimental Parasitology 36, 360–72.CrossRefGoogle Scholar
Stein, L. D. & David, J. R. (1986). Cloning of a developmentally regulated tegument antigen of Schistosoma mansoni. Molecular and Biochemical Parasitology 20, 253–64.CrossRefGoogle ScholarPubMed
Stein, B., Rahmsdorf, H. J., Steffen, A., Litfin, M. & Herrlich, P. (1989). UV-induced DNA damage is an intermediate step in UV-induced expression of human immunodeficiency virus type 1, collagenase, c-fos, and metallothionein. Molecular and Cellular Biology 9, 5169–81.Google ScholarPubMed
Xanthoudokis, S., Miao, G., Wang, F., Pan, Y. C. E. & Curran, T. (1992). Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme. European Molecular Biological Organisation Journal 11, 3323–35.CrossRefGoogle Scholar