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Trichinella spiralis: antigenic epitopes from the stichocytes detected in the hypertrophic nuclei and cytoplasm of the parasitized muscle fibre (nurse cell) of the host

Published online by Cambridge University Press:  06 April 2009

D. L. Lee ,
R. C. Ko ,
X. Y. Yi  and
M. H. F. Yeung
D. L. Lee
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT, UK
R. C. Ko
Affiliation:
Department of Zoology, University of Hong Kong, Hong Kong
X. Y. Yi
Affiliation:
Department of Parasitology, Hunan Medical University, Changsa, Hunan, People's Republic of, China
M. H. F. Yeung
Affiliation:
Department of Zoology, University of Hong Kong, Hong Kong
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Summary

Monoclonal antibodies raised against antigens present in the excretions/secretions (E/S) of larval Trichinella spiralis, polyclonal antibodies raised against E/S and antisera from rabbits and pigs infected with T. spiralis were used in conjunction with immunocytochemical techniques to detect antigens in sections of muscle from mice that had been infected with T. spiralis for 15 or 30 days. The antibodies recognized epitopes in the stichocytes, on the surface of the cuticle, in the lumen of the oesophagus and in the lumen of the intestine of encysted larvae. Monoclonal antibodies 7C2C5 and 1H7 and the polyclonal antibodies recognized epitopes in the cavity occupied by the larva, in the cytoplasm of the nurse cell, and in the hypertrophic nuclei of the nurse cell, but did not recognize material in the smaller nuclei of the nurse cell, in the cyst wall or in the surrounding muscle. Monoclonals 3B2E6 and 1D11G8B2, which recognized epitopes in the stichocytes and on the surface of the cuticle of the larvae, gave negative results with the cytoplasm and nuclei of the nurse cell. A polyclonal antibody raised against Trichuris suis recognized epitopes in the muscle and hypodermis of encysted T. spiralis but gave negative results with material in the nurse cell and nurse cell nuclei. The possibility that the antigen detected in the cytoplasm and nuclei of the nurse cell is produced by the stichocytes of the nematode and that it is controlling genes of the altered muscle fibre, either directly or indirectly, is discussed.

Keywords

antigenepitopemonoclonal antibodynurse cellstichocytesTrichinellaTrichuris
Type
Research Article
Information
Parasitology , Volume 102 , Issue 1 , February 1991 , pp. 117 - 123
Copyright
Copyright © Cambridge University Press 1991

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References

Capo, V., Silberstein, D. S. & Despommier, D. D. (1986). Immunocytolocalization of two protection-inducing antigens of Trichinella spiralis during its enteral phase in immune and non-immune mice. Journal of Parasitology 72, 931–8.CrossRefGoogle ScholarPubMed
Chan, S. W. & Ko, R. C. (1990). Cross-reactions between crude antigens of Trichinella spiralis and other nematodes of Chinese pigs. Tropical Biomedicine (in the Press).Google Scholar
Despommier, D. D. (1983). Biology. In Trichinella and Trichinosis (ed. Campbell, W. C.), pp. 31–73. New York: Plenum Press.Google Scholar
Despommier, D. D. (1990). Trichinella spiralis: the worm that would be a virus. Parasitology Today 6, 193–6.CrossRefGoogle Scholar
Despommier, D. D., Gold, A. M., Buck, S. W., Capo, V. & Silberstein, D. (1990). Trichinella spiralis: secreted antigen of the infective L1 larva localizes to the cytoplasm and nucleoplasm of infected host cells. Experimental Parasitology 71, 27–38.CrossRefGoogle Scholar
Gabryel, P., Gustowska, L., Blotna-Filipiak, M. & Rauhut, W. (1978). Pathomorphology of mouse tissue during Trichinella spiralis infection (light and electron microscopic observations). In Trichinellosis (ed. Kim, C. W. & Pawlowski, Z. S.), pp. 281–94. Hanover, New Hampshire: University Press of New England.Google Scholar
Gamble, H. R. & Graham, C. E. (1984). Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. American Journal of Veterinary Research 45, 67–74.Google ScholarPubMed
Hulinska, D., Grim, M. & Mackinnon, B. M. (1985). Alterations of muscle fibres of mice experimentally infected with Trichinella pseudospiralis, T. spiralis and T. nativa. In Trichinellosis (ed. Kim, C. W.), pp. 157–62. Albany, New York: The State University of New York Press.Google Scholar
Ko, R. C. & Yeung, M. H. F. (1989). Specificity of ES antigens in detection of Trichinella spiralis antibodies in Chinese pigs. Tropical Biomedicine 6, 99–111.Google Scholar
Lee, D. L. & Shivers, R. R. (1987). A freeze-fracture study of muscle fibres infected with Trichinella spiralis. Tissue and Cell 19, 665–71.CrossRefGoogle ScholarPubMed
McLaren, D. J., Ortega-Pierres, G. & Parkhouse, R. M. E. (1987). Trichinella spiralis: immunocytochemical localization of surface and intracellular antigens using monoclonal antibody probes. Parasitology 94, 101–14.CrossRefGoogle ScholarPubMed
Niimura, M., Kobayashi, M. & Kojima, S. (1988). A mouse monoclonal antibody that binds to an α-stichocyte of Trichinella spiralis. Parasitology Research 74, 271–6.CrossRefGoogle Scholar
Polak, J. M. & Van Noorden, S. (1984). An Introduction to Immunochemistry: Current Techniques and Problems. Oxford: Oxford University Press.Google Scholar
Pritchard, D. I. (1985). Antigen production by encysted muscle larvae of Trichinella spiralis. Journal of Helminthology 59, 71–7.CrossRefGoogle ScholarPubMed
Silberstein, D. S. & Despommier, D. D. (1984). Antigens from Trichinella spiralis that induce a protective response in the mouse. Journal of Immunology 132, 898–904.CrossRefGoogle ScholarPubMed
Wright, K. A. (1979). Trichinella spiralis: an intracellular parasite in the intestinal phase. Journal of Parasitology 65, 441–5.CrossRefGoogle ScholarPubMed