Skip to main content Accessibility help
×
Home
Hostname: page-component-55597f9d44-zdfhw Total loading time: 0.558 Render date: 2022-08-08T03:34:51.328Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "useNewApi": true } hasContentIssue true

Effect of a quinoa (Chenopodium quinoa W) based diet on the intestinal mucosa of growing Wistar rats

Published online by Cambridge University Press:  19 April 2013

S. M. Vidueiros
Affiliation:
Department of Nutrition, Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA)
I. Fernandez
Affiliation:
Department of Nutrition, Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA)
D. Bertero
Affiliation:
Department of Vegetal Physiology, Faculty of Agronomy, UBA
M. E. Roux
Affiliation:
ININCA, UBA-CONICET, Argentina
A. Pallaro
Affiliation:
Department of Nutrition, Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA)
Rights & Permissions[Opens in a new window]

Abstract

Type
Abstract
Copyright
Copyright © The Authors 2013 

Quinoa (Chenopodium quínoa W) is a pseudocereal native to the Andean region of South America that has been recognized as an extremely nutritious grain all over the world( Reference Izquierdo, Mujica, Jacobsen and Marathee 1 ). In previous studies, we characterized the nutritional properties of quinoa from Argentinean Northwest( Reference Vidueiros, Dyner, Bertero and Pallaro 2 , Reference Vidueiros, Peterson, Bertero and Pallaro3 ). Moreover, quinoa seeds have a high content of saponins that give them a bitter taste and may alter the intestinal mucosa due to their detergent properties( Reference Izquierdo, Mujica, Jacobsen and Marathee 1 ).

The aim of the present study was to find out the effect of a variety of quinoa from Campo Tapial de Colanzuli, Iruya, Salta, without washing treatment, on the intestinal mucosa of growing rats.

In this study, Wistar rats (n = 6/group) from weaning to 7 days fed a 10% protein diet with 1) unwashed quinoa (Q), 2) commercial washed quinoa (QR), and 3) casein (C) as control group. Body weight (BW, g) and diet intake (I, g/day) were determined, and Ponderal Growth Rate (PGR, g/day/100g) was calculated. Intestines were removed, processed by Saint Marie´s technique and stained with Alcian Blue-H/E. Goblets cells/100 epithelial cells (GC) were determined in 10 intestinal villi/rat. Tissue sections were studied by indirect immunofluorescence technique4). IL-17+ cells, CD5+ T cells and TCRγδ+ subset levels were measured in lamina propria (LP) and intraepithelium (iIEL). IgA-B+ cells were determined in LP by reading the number of cells/30 fields.

Results are shown in the table below.

Means within rows with different superscript, differ 0.05 > p < 0.001 (ANOVA, followed by SNK test).

No differences were observed in IgA-B+ cells, CD5+ T cells and TCRγδ+ subset among groups. Moreover, goblets cells and IL-17+ cells were significant increased in Q group which could indicate an inflammatory process caused by the saponins from unwashed quinoa. This could affect the normal intestinal absorption of nutrients which is reflected in BW, PGR and I, compared to QR group.

This work was supported by UBACyT 20020100100255 and 20620100100014.

References

1. Izquierdo, J, Mujica, A, Jacobsen, S, Marathee, JP, (2001) FAO, Santiago (Chile). Oficina Regional para América Latina y el Caribe.Google Scholar
2. Vidueiros, SM, Dyner, L, Bertero, D & Pallaro, A (2010) FASEB J 24, 949.2 Google Scholar
3. Vidueiros, SM, Peterson, G, Bertero, D & Pallaro, A (2011) FASEB J 25, 770.16.Google Scholar
4. Vidueiros, SM, Fernandez, I, Slobodianik, N, Roux, ME & Pallaro, A (2008). Nutrition 24, 575581.CrossRefGoogle ScholarPubMed
Figure 0

You have Access

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Effect of a quinoa (Chenopodium quinoa W) based diet on the intestinal mucosa of growing Wistar rats
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Effect of a quinoa (Chenopodium quinoa W) based diet on the intestinal mucosa of growing Wistar rats
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Effect of a quinoa (Chenopodium quinoa W) based diet on the intestinal mucosa of growing Wistar rats
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *