Skip to main content Accessibility help
×
Home
Hostname: page-component-59b7f5684b-vcb8f Total loading time: 0.882 Render date: 2022-09-27T04:55:38.757Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "useRatesEcommerce": false, "displayNetworkTab": true, "displayNetworkMapGraph": false, "useSa": true } hasContentIssue true

Effects of broccoli sprouts intake on oxidative stress, inflammation, microalbuminuria and platelet function in human volunteers: a cross-over study

Published online by Cambridge University Press:  08 April 2011

E. Munters
Affiliation:
Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium
N. Pieters
Affiliation:
Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium
A. Cuypers
Affiliation:
Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium
J. Penders
Affiliation:
Hospital ZOL, Genk, Belgium Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium
J. Vangronsveld
Affiliation:
Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium
T. Nawrot
Affiliation:
Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium
Rights & Permissions[Opens in a new window]

Abstract

Type
Abstract
Copyright
Copyright © The Authors 2011

Broccoli sprouts contain a high content of glucosinolates(Reference Shapiro, Fahey and Wade1). These substances induce phase 2 enzymes, known for their protection against carcinogenesis(Reference Verhagen, de Vries and Nijhoff2), mutagenesis and other forms of toxicity of electrophiles and reactive forms of oxygen. We investigated the effects of the intake of broccoli sprouts on inflammation, microalbuminuria, platelet function and oxidative DNA damage.

The study included 23 patients aged between 22 and 35 years who had no history of chronic illnesses or other harmful diseases. They were randomised in a cross-over study with two treatments: 20 g broccoli sprouts or no broccoli sprouts for 4 d. The two groups were crossed after 2 weeks. Platelet function was measured ex vivo with the PFA-100 platelet function analyser, which simulates a damaged blood vessel; we analysed the function of platelets in primary haemostasis under high shear conditions. Total and differential blood leucocytes were counted and oxidative DNA damage measured (8-hydroxy-2-deoxyguanosine [8-OH-2dG]) in urine from healthy volunteers.

Baseline values were: lymphocytes (1000 μl1) 2.13±0.44; monocytes (%) 5.90±1.07; 8-OH-2dG/creatinine (μg/g) 1.09±0.28 and microalbumine/creatinine (μg/g) 0.55±0.29. These variations induced by broccoli sprout intake showed a 8.9% reduction (P=0.029) in the amount of lymphocytes, a 11.9% reduction (P=0.0093) in the percentage of monocytes, a 34.5% reduction (P=0.016) in microalbumine/creatinine concentration and a 16.7% increase (P=0.012) in 8-OH-2dG/creatinine concentration. The intake of broccoli sprouts did not significantly influence platelet function action. Non-significant results were found in the assessment of blood and urine content in the control group.

Broccoli sprouts intake showed a significant reduction of lymphocytes, monocytes and microalbuminuria and a significant increase of 8-OH-2dG. Whether the increase in urinary 8-OH-2dG reflects enhanced DNA repair or more repair because of increased oxidative DNA damage by broccoli sprouts must be further elucidated.

References

1.Shapiro, TA, Fahey, JW, Wade, KL et al. (2001) Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts: metabolism and excretion in humans. Cancer Epidemiol Biomarkers Prev 10(5), 501508.Google Scholar
2.Verhagen, H, de Vries, A, Nijhoff, WA et al. (1997) Effect of Brussels sprouts on oxidative DNA-damage in man. Cancer Lett 114(1–2), 127130.CrossRefGoogle Scholar
You have Access
2
Cited by

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Effects of broccoli sprouts intake on oxidative stress, inflammation, microalbuminuria and platelet function in human volunteers: a cross-over study
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Effects of broccoli sprouts intake on oxidative stress, inflammation, microalbuminuria and platelet function in human volunteers: a cross-over study
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Effects of broccoli sprouts intake on oxidative stress, inflammation, microalbuminuria and platelet function in human volunteers: a cross-over study
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *