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Effects of green tea, black tea and Rooibos tea on angiotensin-converting enzyme and nitric oxide in healthy volunteers

  • Ingrid A-L Persson (a1), Karin Persson (a1), Staffan Hägg (a2) and Rolf GG Andersson (a1)
Abstract
AbstractObjective

Tea has been reported to reduce cardiovascular mortality, but the underlying mechanisms are largely unknown. The aim of the current project was to investigate the effect of green tea (Japanese Sencha), black tea (Indian Assam B.O.P.) and Rooibos tea (South Africa) on angiotensin-converting enzyme (ACE) and nitric oxide (NO).

Design

Seventeen healthy volunteers received a single oral dose of 400 ml green tea, black tea or Rooibos tea in a randomized, three-phase, crossover study. ACE activity and NO concentration were measured (at 0, 30, 60 and 180 min) in all phases. ACE activity was analysed by means of a commercial radioenzymatic assay. Nitrite was analysed as a marker of NO concentration. In addition, ACE genotype was determined using a PCR method.

Results

Oral intake of a single dose of Rooibos tea significantly inhibited ACE activity after 30 min (P < 0·01) and after 60 min (P < 0·05). A significant inhibition of ACE activity was seen with green tea for the ACE II genotype 30 min after intake of the tea (P < 0·05) and for the ACE ID genotype 60 min after intake (P < 0·05). A significant inhibition of ACE activity was also seen with Rooibos tea for the ACE II genotype 60 min after intake (P < 0·05). No significant effect on NO concentration was seen.

Conclusions

These results suggest that green tea and Rooibos tea may have cardiovascular effects through inhibition of ACE activity.

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*Corresponding author: Email ingrid.persson@liu.se
References
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1. DA Balentine , SA Wiseman & LCM Bouwens (1997) The chemistry of tea flavonoids. Crit Rev Food Sci Nutr 37, 693704.

2. IA Hakim , MA Alsaif , M Alduwaihy (2003) Tea consumption and the prevalence of coronary heart disease in Saudi adults: results from a Saudi national study. Prev Med 36, 6470.

3. KJ Mukamal , M Maclure , JE Muller (2002) Tea consumption and mortality after acute myocardial infarction. Circulation 105, 24762481.

4. A Basu & EA Lucas (2007) Mechanisms and effects of green tea on cardiovascular health. Nutr Rev 65, 361375.

5. S Inami , M Takano , M Yamamoto (2008) Tea catechin consumption reduces circulating oxidized low-density lipoprotein. Int Heart J 48, 725732.

6. JH O’Keefe , NM Gheewala & J O’Keefe (2008) Dietary strategies for improving post-prandial glucose, lipids, inflammation, and cardiovascular health. J Am Coll Cardiol 51, 249255.

7. GL Tipoe , TM Leung , MW Hung (2007) Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection. Cardiovasc Hematol Disord Drug Targets 7, 135144.

8. SK Kim , HJ Kim , SE Choi (2008) Anti-oxidative and inhibitory activities on nitric oxide (NO) and prostaglandin E2 (COX-2) production of flavonoids from seeds of Prunus tomentosa Thunberg. Arch Pharm Res 31, 424428.

9. S Ahmed , A Rahman , A Hasnain (2002) Green tea polyphenol epigallocatechin-3-gallate inhibits the IL-1β-induced activity and expression of cyclooxygenase-2 and nitric oxide synthase-2 in human chondrocytes. Free Radic Biol Med 33, 10971105.

11. IA-L Persson , M Josefsson , K Persson (2006) Tea flavanols inhibit angiotensin-converting enzyme activity and increase nitric oxide production in human endothelial cells. J Pharm Pharmacol 58, 11391144.

12. L Bramati , F Aquiland & P Pietta (2003) Unfermented Rooibos tea. Quantitative characterization of flavonoids by HPLC-UV and determination of the total antioxidant activity. J Agric Food Chem 51, 74727474.

13. H Nishimura (2000) Angiotensin receptors – evolutionary overview and perspectives. Comp Biochem Physiol 128, 1130.

14. SC Benson , R Iguchi , CI Ho (2008) Inhibition of cardiovascular cell proliferation by angiotensin receptor blockers: are all molecules the same? J Hypertens 26, 973980.

15. R Fogari & A Zoppi (2006) Antihypertensive drugs and fibrinolytic function. Am J Hypertens 19, 12931299.

16. JW Rush & CD Aultman (2008) Vascular biology of angiotensin and the impact of physical activity. Appl Physiol Nutr Metab 33, 162172.

17. D Weiss , D Sorescu & WR Taylor (2001) Angiotensin II and atherosclerosis. Am J Cardiol 87, 25C32C.

18. WH Birkenhäger & JA Staessen (2006) Progress in cardiovascular diseases: cognitive function in essential hypertension. Prog Cardiovasc Dis 49, 110.

19. PR Gard (2002) The role of angiotensin II in cognition and behavior. Eur J Pharmacol 438, 114.

21. R Reed , C Kolz , B Potter (2008) The mechanistic basis for the disparate effects of angiotensin II on coronary collateral growth. Arterioscler Thromb Vasc Biol 28, 6167.

22. B Rigat , C Hubert , F Alhenc-Gelas (1990) An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest 86, 13431346.

25. F Cambien , O Poirier , L Lecerf (1992) Deletion polymorphism in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction. Nature 359, 641644.

26. NJ Samani , JR Thompson , L O’Toole (1996) A meta-analysis of the association of the deletion allele of the angiotensin-converting enzyme gene with myocardial infarction. Circulation 94, 708712.

27. LJ Millatt , EM Abdel-Rahman & HM Siragy (1999) Angiotensin II and nitric oxide: a question of balance. Regul Pept 81, 110.

28. MS Fernándes-Alfonso & C González (1999) Nitric oxide and the renin–angiotensin system. Is there a physiological interplay between the systems? J Hypertens 17, 13551361.

29. S Kurz , U Hink , G Nickenig (1999) Evidence for a causal role of the renin–angiotensin system in nitrate tolerance. Circulation 99, 31813187.

30. A Ackermann , MS Fernández-Alfonso , R Sánchez de Rojas (1998) Modulation of angiotensin-converting enzyme by nitric oxide. Br J Pharmacol 124, 291298.

31. K Persson & RGG Andersson (1999) Nitric oxide modulates captopril-mediated angiotensin-converting enzyme inhibition in porcine iliac arteries. Eur J Pharmacol 385, 2127.

32. K Persson , PW Whiss , K Nyhlén (2000) Nitric oxide donors and angiotensin-converting enzyme inhibitors act in concert to inhibit human angiotensin-converting enzyme activity and platelet aggregation in vitro. Eur J Pharmacol 406, 1523.

33. T Lauer , M Preik , T Rassaf (2001) Plasma nitrite rather than nitrate reflects regional endothelial nitric oxide synthase activity but lacks intrinsic vasodilator action. Proc Natl Acad Sci USA 98, 1281412819.

34. KT Cheon , KH Choi , HB Lee (2000) Gene polymorphisms of endothelial nitric oxide synthase and angiotensin-converting enzyme in patients with lung cancer. Lung 178, 351360.

35. S Karakaya (2004) Bioavailability of phenolic compounds. Crit Rev Food Sci Nutr 44, 453464.

36. F Cambien (1994) The angiotensin-converting enzyme (ACE) genetic polymorphism: its relationship with plasma ACE level and myocardial infarction. Clin Genet 46, 94101.

37. K Persson , ACE Säfholm , RGG Andersson (2005) Glyceryl trinitrate-induced angiotensin-converting enzyme (ACE) inhibition in healthy volunteers is dependent on ACE genotype. Can J Physiol Pharmacol 83, 11171122.

38. AHJ Danser , WW Batenburg , AH van den Meiracker (2007) ACE phenotyping as a first step toward personalized medicine for ACE inhibitors. Why does ACE genotyping not predict the therapeutic efficacy of ACE inhibitors? Pharmacol Ther 113, 607618.

40. K Berecek & L Zhang (1995) Biochemistry and cell biology of angiotensin-converting enzyme and converting enzyme inhibitors. Adv Exp Med Biol 377, 141168.

42. D Grassi , TPJ Mulder , R Draijer (2009) Black tea consumption dose-dependently improves flow-mediated dilation in healthy males. J Hypertens 27, 774781.

45. CM Chen , W Zhao , Z Yang (2008) The role of dietary factors in chronic disease control in China. Obes Rev 9, Suppl. 1, 100103.

46. EP López , C Rice , DO Weddle (2008) The relationship among cardiovascular risk factors, diet patterns, alcohol consumption, and ethnicity among women aged 50 years and older. J Am Diet Assoc 108, 248256.

47. C Patterson , JW Feightner , A Garcia (2008) Diagnosis and treatment of dementia: risk assessment and primary prevention of Alzheimer disease. CMAJ 178, 548556.

48. LO Dragsted (2003) Antioxidant actions of polyphenols in humans. Int J Vitam Nutr Res 73, 112119.

49. PM Aron & JA Kennedy (2008) Flavan-3-ols: nature, occurrence and biological activity. Mol Nutr Food Res 52, 79104.

50. AW Boots , GRMM Haenen & A Bast (2008) Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol 585, 325337.

51. J Hwang , J Wang , P Morazzoni (2003) The phytoestrogen equol increases nitric oxide availability by inhibiting superoxide production: an antioxidant mechanism for cell-mediated LDL modification. Free Radic Biol Med 34, 12711282.

53. AC Kaliora & GVZ Dedoussis (2007) Natural antioxidant compounds in risk factors for CVD. Pharmacol Res 56, 99109.

54. I Svarcova , J Heinrich & K Valentova (2007) Berry fruits as a source of biologically active compounds: the case of Lonicera caerulea. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 151, 163174.

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