Skip to main content
×
Home
    • Aa
    • Aa

Imaging the adult zebrafish cone mosaic using optical coherence tomography

  • ALISON L. HUCKENPAHLER (a1), MELISSA A. WILK (a1), ROBERT F. COOPER (a2) (a3), FRANCIE MOEHRING (a1), BRIAN A. LINK (a1), JOSEPH CARROLL (a1) (a4) and ROSS F. COLLERY (a1)...
Abstract
Abstract

Zebrafish (Danio rerio) provide many advantages as a model organism for studying ocular disease and development, and there is great interest in the ability to non-invasively assess their photoreceptor mosaic. Despite recent applications of scanning light ophthalmoscopy, fundus photography, and gonioscopy to in vivo imaging of the adult zebrafish eye, current techniques either lack accurate scaling information (limiting quantitative analyses) or require euthanizing the fish (precluding longitudinal analyses). Here we describe improved methods for imaging the adult zebrafish retina using spectral domain optical coherence tomography (OCT). Transgenic fli1:eGFP zebrafish were imaged using the Bioptigen Envisu R2200 broadband source OCT with a 12-mm telecentric probe to measure axial length and a mouse retina probe to acquire retinal volume scans subtending 1.2 × 1.2 mm nominally. En face summed volume projections were generated from the volume scans using custom software that allows the user to create contours tailored to specific retinal layer(s) of interest. Following imaging, the eyes were dissected for ex vivo fluorescence microscopy, and measurements of blood vessel branch points were compared to those made from the en face OCT images to determine the OCT lateral scale as a function of axial length. Using this scaling model, we imaged the photoreceptor layer of five wild-type zebrafish and quantified the density and packing geometry of the UV cone submosaic. Our in vivo cone density measurements agreed with measurements from previously published histology values. The method presented here allows accurate, quantitative assessment of cone structure in vivo and will be useful for longitudinal studies of the zebrafish cone mosaics.

  • View HTML
    • Send article to Kindle

      To send this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle.

      Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

      Find out more about the Kindle Personal Document Service.

      Imaging the adult zebrafish cone mosaic using optical coherence tomography
      Available formats
      ×
      Send article to Dropbox

      To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about sending content to Dropbox.

      Imaging the adult zebrafish cone mosaic using optical coherence tomography
      Available formats
      ×
      Send article to Google Drive

      To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about sending content to Google Drive.

      Imaging the adult zebrafish cone mosaic using optical coherence tomography
      Available formats
      ×
Copyright
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
*Address correspondence to: Ross Collery, PhD, Department of Cell Biology, Neurobiology, and Anatomy; Medical College of Wisconsin, 8701 W. Watertown Plank Rd, Milwaukee, WI 53226-0509. E-mail: rcollery@mcw.edu
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

T.J.Bailey , D.H.Davis , J.E.Vance & D.R.Hyde (2012). Spectral-domain optical coherence tomography as a noninvasive method to assess damaged and regenerating adult zebrafish retinas. Investigative Ophthalmology & Visual Science 53, 31263138.

T.Branchek & R.Bremiller (1984). The development of photoreceptors in the zebrafish, Brachydanio rerio. I. Structure. Journal of Comparative Neurology 224, 107115.

Y.H.Cheng , J.Y.Yu , H.H.Wu , B.J.Huang & S.W.Chu (2010). Spectral ophthalmoscopy based on supercontinuum. In Proceedings of SPIE, ed. D.L.Farkas , D.V.Nicolau & R.C.Leif , pp. 75680F. San Francisco, CA: SPIE.

S.J.Chiu , Y.Lokhnygina , A.M.Dubis , A.Dubra , J.Carroll , J.A.Izatt & S.Farsiu (2013). Automatic cone photoreceptor segmentation using graph theory and dynamic programming. Biomedical Optics Express 4, 924937.

R.F.Cooper , M.A.Wilk , S.Tarima & J.Carroll (2016). Evaluating descriptive metrics of the human cone mosaic. Investigative Ophthalmology & Visual Science 57(7), 29923001.

J.A.Flatter , R.F.Cooper , M.J.Dubow , A.Pinhas , R.S.Singh , R.Kapur , N.Shah , R.D.Walsh , S.H.Hong , D.V.Weinberg , K.E.Stepien , W.J.Wirostko , S.Robison , A.Dubra , R.B.Rosen , T.B.ConnorJr. & J.Carroll (2014). Outer retinal structure after closed-globe blunt ocular trauma. Retina 34, 21332146.

B.Fraser , M.G.Duval , H.Wang & W.T.Allison (2013). Regeneration of cone photoreceptors when cell ablation is primarily restricted to a particular cone subtype. PLoS One 8, e55410.

R.Garrioch , C.Langlo , A.M.Dubis , R.F.Cooper , A.Dubra & J.Carroll (2012). Repeatability of in vivo parafoveal cone density and spacing measurements. Optometry and Vision Science 89, 632643.

M.K.Garvin , M.D.Abramoff , X.Wu , S.R.Russell , T.L.Burns & M.Sonka (2009). Automated 3-D intraretinal segmentation of macular spectral-domain optical coherence tomography images. IEEE Transactions on Medical Imaging 28, 14361447.

A.Hayashi , A.Naseri , M.E.Pennesi & E.J.de Juan (2009). Subretinal delivery of immunoglobulin G with gold nanoparticles in the rabbit eye. Japanese Journal of Ophthalmology 53, 249256.

D.C.Hood , B.Fortune , M.A.Mavrommatis , J.Reynaud , R.Ramachandran , R.Ritch , R.B.Rosen , H.Muhammad , A.Dubra & T.Y.Chui (2015). Details of glaucomatous damage are better seen on OCT en face images than on OCT retinal nerve fiber layer thickness maps. Investigative Ophthalmology & Visual Science 56, 62086216.

B.A.Link & R.F.Collery (2015). Zebrafish models of retinal disease. Annual Review of Vision Science 1, 125153.

R.Lu , A.M.Levy , Q.Zhang , S.J.Pittler & X.Yao (2013). Dynamic near-infrared imaging reveals transient phototropic change in retinal rod photoreceptors. Journal of Biomedical Optics 18, 106013.

D.M.Mitchell , C.B.Stevens , R.A.Frey , S.S.Hunter , R.Ashino , S.Kawamura & D.L.Stenkamp (2015). Retinoic acid signalling regulates differential expression of the tandemly-duplicated long wavelength-sensitive cone opsin genes in zebrafish. PLoS Genetics 11, e1005483.

D.Odell , A.M.Dubis , J.F.Lever , K.E.Stepien & J.Carroll (2011). Assessing errors inherent in OCT-derived macular thickness maps. Journal of Ophthalmology 2011, 692574.

M.A.Pickart & E.W.Klee (2014). Zebrafish approaches enhance the translational research tackle box. Translational Research 163, 6578.

J.Robinson , E.A.Schmitt , F.I.Harosi , R.J.Reece & J.E.Dowling (1993). Zebrafish ultraviolet visual pigment: Absorption spectrum, sequence, and localization. Proceedings of the National Academy of Sciences of the United States of America 90, 60096012.

C.A.Schneider , W.S.Rasband & K.W.Eliceiri (2012). NIH image to ImageJ: 25 years of image analysis. Nature Methods 9, 671675.

D.Scoles , J.A.Flatter , R.F.Cooper , C.S.Langlo , S.Robison , M.Neitz , D.V.Weinberg , M.E.Pennesi , D.P.Han , A.Dubra & J.Carroll (2016). Assessing photoreceptor structure associated with ellipsoid zone disruptions visualized with optical coherence tomography. Retina 36, 91103.

M.Tschopp , M.Takamiya , K.L.Cerveny , G.Gestri , O.Biehlmaier , S.W.Wilson , U.Strähle & S.C.Neuhauss (2010). Funduscopy in adult zebrafish and its application to isolate mutant strains with ocular defects. PLoS One 5, e15427.

J.Wan & D.Goldman (2016). Retina regeneration in zebrafish. Current Opinion in Genetics & Development 40, 4147.

K.Watanabe , Y.Nishimura , T.Oka , T.Nomoto , T.Kon , T.Shintou , M.Hirano , Y.Shimada , N.Umemoto , J.Kuroyanagi , Z.Wang , Z.Zhang , N.Nishimura , T.Miyazaki , T.Imamura & T.Tanaka (2010). In vivo imaging of zebrafish retinal cells using fluorescent coumarin derivatives. BMC Neuroscience 11, 116.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Visual Neuroscience
  • ISSN: 0952-5238
  • EISSN: 1469-8714
  • URL: /core/journals/visual-neuroscience
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Altmetric attention score