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Cytological Effects of Ultra-High Temperatures on Corn

Published online by Cambridge University Press:  12 June 2017

T. C. Ellwanger Jr. ,
S. W. Bingham ,
W. E. Chappell  and
S. A. Tolin
T. C. Ellwanger Jr.
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
S. W. Bingham
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
W. E. Chappell
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
S. A. Tolin
Affiliation:
Dep. of Plant Path. and Virginia Polytech. Inst. and State Univ., Blacksburg, Va. 24061
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Abstract

Corn (Zea mays L. ‘Funk's G-83’) seedling leaves exposed to flame-generated ultra-high temperatures produced in flame cultivation were fixed in glutaraldehyde, post fixed in osmium tetroxide, and embedded in Araldite. In the light microscope, bundle sheath cells of flamed tissue were more heavily stained with Azure II and less vacuolated than were nonflamed cells. Heated mesophyll cells contained swollen, disrupted, and granular chloroplasts. Examination of flamed tissue by electron microscopy revealed granular, dispersed cytaplasm and altered membrane systems. Chloroplast lamellar systems and envelopes, tonoplasts, and plasmalemmas were disintegrated in both bundle sheath and mesophyll cells.

Information

Type
Research Article
Information
Weed Science , Volume 21 , Issue 4 , July 1973 , pp. 299 - 303
Copyright
Copyright © 1973 Weed Science Society of America 

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References

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