Skip to main content
×
Home
    • Aa
    • Aa

Assessment of two nondestructive assays for detecting glyphosate resistance in horseweed (Conyza canadensis)

  • Clifford H. Koger, Dale L. Shaner (a1), W. Brien Henry (a2), Talia Nadler-Hassar (a3), Walter E. Thomas (a4) and John W. Wilcut (a4)...
Abstract

Two rapid, nondestructive assays were developed and tested for their potential in differentiating glyphosate-resistant from glyphosate-susceptible biotypes of horseweed. In one assay, leaves of glyphosate-resistant and -susceptible corn, cotton, and soybean plants, as well as glyphosate-resistant and -susceptible horseweed plants, were dipped in solutions of 0, 300, 600, and 1,200 mg ae L−1 glyphosate for 3 d, and subsequent injury was evaluated. In the second assay, plant sensitivity to glyphosate was evaluated in vivo by incubating excised leaf disc tissue from the same plants used in the first assay in 0.7, 1.3, 2.6, 5.3, 10.6, 21.1, 42.3, and 84.5 mg ae L−1 glyphosate solutions for 16 h and measuring shikimate levels with a spectrophotometer. The leaf dip assay differentiated between glyphosate-resistant and -susceptible crops and horseweed biotypes. The 600 mg L−1 rate of glyphosate was more consistent in differentiating resistant and susceptible plants compared with the 300 and 1,200 mg L−1 rates. The in vivo assay detected significant differences between susceptible and glyphosate-resistant plants of all species. Shikimate accumulated in a glyphosate dose–dependent manner in leaf discs from susceptible crops, but shikimate did not accumulate in leaf discs from resistant crops, and levels were similar to nontreated leaf discs. Shikimate accumulated at high (≥ 21.1 mg ae L−1) concentrations of glyphosate in leaf discs from all horseweed biotypes. Shikimate accumulated at low glyphosate concentrations (≤ 10.6 mg L−1) in leaf discs from susceptible horseweed biotypes but not in resistant biotypes. Both assays were able to differentiate resistant from susceptible biotypes of horseweed and could have utility for screening other weed populations for resistance to glyphosate.

Copyright
Corresponding author
Corresponding author. U.S. Department of Agriculture–Agricultural Research Service (USDA–ARS), Crop Genetics and Production Research Unit and Southern Weed Science Research Unit, P.O. Box 350, Stoneville, MS 38776; ckoger@ars.usda.gov
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

S. R. Baerson , D. J. Rodriguez , M. Tan , Y. Feng , N. A. Biest , and G. M. Dill 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129:12651275.

H. J. Beckie , I. M. Heap , R. J. Smeda , and L. M. Hall 2000. Screening for herbicide resistance in weeds. Weed Technol 14:428445.

R. E. Blackshaw and K. N. Harker 2002. Selective weed control with glyphosate in glyphosate-resistant spring wheat (Triticum aestivum). Weed Technol 16:885892.

P. Boutsalis 2001. Syngenta quick-test: a rapid whole-plant test for herbicide resistance. Weed Technol 15:257263.

G. A. Bresnahan , F. A. Manthey , K. A. Howatt , and M. Chakraborty 2003. Glyphosate applied preharvest induces shikimic acid accumulation in hard red spring wheat (Triticum aestivum). J. Agric. Food Chem 51:40044007.

D. Chachalis , K. N. Reddy , C. D. Elmore , and M. L. Steele 2001. Herbicide efficacy, leaf structure, and spray droplet contact angle among Ipomoea species and smallflower morningglory. Weed Sci 49:628634.

M. C. Escorial , H. Sixto , J. M. Gracia-Baudin , and M. C. Chueca 2001. A rapid method to determine cereal plant response to glyphosate. Weed Technol 15:697702.

W. H. Faircloth , M. G. Patterson , C. D. Monks , and W. R. Goodman 2001. Weed management programs for glyphosate-tolerant cotton (Gossypium hirsutum). Weed Technol 15:544551.

P. C. C. Feng , M. Tran , T. Chiu , R. D. Sammons , G. R. Heck , and C. A. CaJacob 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci 52:498505.

S. A. Gower , M. M. Loux , and J. Cardina et al. 2003. Effect of postemergence glyphosate application timing on weed control and grain yield in glyphosate-resistant corn: results of a 2-yr multistate study. Weed Technol 17:821828.

C. H. Koger , D. H. Poston , and K. N. Reddy 2004b. Effect of glyphosate spray coverage on control of pitted morningglory (Ipomoea lacunosa). Weed Technol 18:124130.

C. H. Koger , A. J. Price , and K. N. Reddy 2005. Weed control and cotton (Gossypium hirsutum) response to combinations of glyphosate and trifloxysulfuron. Weed Technol 19:113121.

C. H. Koger and K. N. Reddy 2005. Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis). Weed Sci 53:8489.

L. J. Lee and J. Ngim 2000. A first report of glyphosate-resistant goosegrass (Eleusine indica (L.) Gaertn) in Malaysia. Pest Manag. Sci 56:336339.

D. F. Lorraine-Colwill , S. B. Powles , T. R. Hawkes , P. H. Hollinshead , S. A. J. Warner , and C. Preston 2003. Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic. Biochem. Physiol 74:6272.

C. L. Main , V. R. Pantalone , and T. C. Mueller 2004. A novel approach to determine the glyphosate tolerant trait in soybeans. J. Agric. Food Chem 52:12241227.

T. C. Mueller , J. H. Massey , R. M. Hayes , C. L. Main , and C. N. Stewart Jr. 2003. Shikimate accumulation in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq). J. Agric. Food Chem 51:680684.

A. Perez and M. Kogan 2003. Glyphosate-resistant Lolium multiflorum in Chilean orchards. Weed Res 43:1219.

W. A. Pline , J. W. Wilcut , S. O. Duke , K. L. Edmisten , and R. Wells 2002. Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate-resistant and nonglyphosate-resistant cotton (Gossypium hirsutum L). J. Agric. Food Chem 50:506512.

G. H. Scott , S. D. Askew , and J. W. Wilcut 2002. Glyphosate systems for weed control in glyphosate-tolerant cotton (Gossypium hirsutum). Weed Technol 16:191198.

D. L. Shaner , T. Nadler-Hassar , W. B. Henry , and C. H. Koger 2005. A rapid in vivo EPSPS assay with excised leaf discs. Weed Technol. In press.

D. R. Shaw and J. C. Arnold 2002. Weed control from herbicide combinations with glyphosate. Weed Technol 16:16.

M. J. VanGessel 2001. Glyphosate-resistant horseweed from Delaware. Weed Sci 49:703705.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Abstract views

Total abstract views: 49 *
Loading metrics...

* Views captured on Cambridge Core between 20th January 2017 - 21st September 2017. This data will be updated every 24 hours.