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The Basis for Glyphosate Resistance in Rigid Ryegrass (Lolium rigidum) from California

  • Marulak Simarmata (a1) and Donald Penner (a1)
Abstract

The occurrence of glyphosate-resistant weeds has been reported after more than 20 yr of extensive use. Rigid ryegrass that evolved resistance to glyphosate was found in Australia and in California. Glyphosate-resistant rigid ryegrass plants were collected from northern California and selected through generations 8 and 5 to segregate the most resistant (R) and sensitive (S) biotypes. The eighth generation of R and the fifth generation of S biotypes survived 6.72 and died from 0.11 kg ae ha−1 glyphosate, respectively. The objectives of this study were to evaluate the role of metabolism in the observed resistance, to study the effect of glyphosate on the activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), and to characterize the EPSPS gene in R and S rigid ryegrass. Neither quantitative nor qualitative difference was observed in the metabolism of 14C-glyphosate between the biotypes. Activity of constitutive EPSPS decreased more significantly in the S than R biotype in the presence of 5, 50, 500, and 5,000 µM glyphosate. Inhibition of 50% (I50) of the EPSPS activity by glyphosate was more than 90-fold in S compared to R biotype. Decreased EPSPS sensitivity in the R biotype appeared to be a major contributor to glyphosate resistance in rigid ryegrass from California. Fragments of the EPSPS gene containing 1,320 nucleotides were isolated from mRNA of S and R biotypes. A single nucleotide mutation from cytosine (C) to thymine (T) was identified at nucleotide 301 of the truncated EPSPS gene of the R biotype. This mutation changed the amino acid code from proline (Pro) to serine (Ser), which was similar to that reported for the glyphosate-resistant goosegrass from Malaysia and correlated with glyphosate insensitivity of EPSPS.

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Corresponding author's E-mail: pennerd@msu.edu
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S. R. Baerson , D. J. Rodriguez , M. Tran , J. You , R. W. Kreuger , G. M. Dill , J. E. Pratley , and K. J. Gruys 2002a. Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum). Weed Sci. 50:721730.

S. R. Baerson , D. J. Rodriguez , M. Tran , Y. Feng , N. A. Biest , and G. M. Dill 2002b. Glyphosate resistance goosegrass: identification of mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:12651275.

M. M. Bradford 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem. 72:248254.

P. Chomczynski and N. Sacchi 1987. Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156159.

R. H. Don , P. T. Cox , B. J. Wainwright , K. Baker , and J. S. Mattick 1991. Touchdown PCR circumvents spurious priming during gene amplification. Nucleic Acids Res. 19 (14):4008.

P. C. C. Feng , M. Tran , T. Chiu , R. D. Sammons , G. R. Heck , and C. A. CaJacob 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci. 52:498505.

D. F. Lorraine-Colwill , T. R. Hawkes , P. H. Williams , S. A. J. Warner , P. B. Sutton , S. B. Powles , and C. Preston 1999. Resistance to glyphosate in Lolium rigidum . Pestic. Sci. 55:486503.

D. F. Lorraine-Colwill , S. B. Powles , T. R. Hawkes , P. H. Hollinshead , S. A. J. Warner , and C. Preston 2003. Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic. Biochem. Physiol. 74:6272.

C. H. Ng , R. Wickneswari , S. Salmijah , Y. T. Teng , and B. Ismail 2003. Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. Weed Res. 43:107115.

A. Perez-Jones , K. W. Park , J. Colquhoun , C. Mallory-Smith , and D. Shaner 2005. Identification of glyphosate-resistant Italian ryegrass (Lolium multiflorum) in Oregon. Weed Sci. 53:775779.

C. L. Sandberg , W. F. Meggitt , and D. Penner 1980. Absorption, translocation, and metabolism of 14C-glyphosate in several weed species. Weed Res. 20:195200.

M. Simarmata , J. E. Kaufmann , and D. Penner 2003. Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum). Weed Sci. 51:678682.

A. M. Wakelin and C. Preston 2006. A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432440.

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Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
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