Hostname: page-component-7dd5485656-wlg5v Total loading time: 0 Render date: 2025-10-31T05:25:13.611Z Has data issue: false hasContentIssue false
  • English
  • Français

Confirmation of a Three-Way (Glyphosate, ALS, and Atrazine) Herbicide-Resistant Population of Palmer Amaranth (Amaranthus palmeri) in Michigan

Published online by Cambridge University Press:  19 April 2017

Jonathon R. Kohrt ,
Christy L. Sprague ,
Satya Swathi Nadakuduti  and
David Douches
Jonathon R. Kohrt
Affiliation:
Graduate Student, Professor, Postdoctoral Research Associate, and Professor, Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824
Christy L. Sprague*
Affiliation:
Graduate Student, Professor, Postdoctoral Research Associate, and Professor, Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824
Satya Swathi Nadakuduti
Affiliation:
Graduate Student, Professor, Postdoctoral Research Associate, and Professor, Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824
David Douches
Affiliation:
Graduate Student, Professor, Postdoctoral Research Associate, and Professor, Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824
*
*Corresponding author’s E-mail: sprague1@msu.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

The failure of PRE and POST applications of atrazine to control Palmer amaranth in recent field studies prompted further investigation to determine whether this population had evolved resistance to multiple herbicide sites of action, including glyphosate (Group 9), thifensulfuron (Group 2), and atrazine (Group 5). Greenhouse and laboratory experiments were conducted to: (1) confirm the presence of resistance to glyphosate, an ALS inhibitor (thifensulfuron), and atrazine in a single Palmer amaranth population; and (2) establish the molecular basis for resistance to these herbicide sites of action. In the greenhouse, glyphosate+thifensulfuron+atrazine at 1.26 kg ae ha−1+0.0044 kg ai ha−1+1.12 kg ai ha−1 provided 55% control of the suspected multiply resistant (MR) Palmer amaranth population and 93% control of the known susceptible population (S). The decreased sensitivity of the MR population compared with the S population at labeled use rates of these herbicides indicated that this population was likely resistant to three different herbicide site of action groups. The RF values for POST applications of glyphosate, thifensulfuron, and atrazine were 12.2, 42.9, and 9.3 times, respectively, for the MR Palmer amaranth population relative to the S population. The RF value for atrazine PRE for the MR population was 112.2 times. Laboratory experiments confirmed that the mechanisms for resistance to ALS-inhibiting herbicides and glyphosate in the MR Palmer amaranth population were target-site based, via amino acid substitution and amplified EPSPS copy number, respectively. There was a Pro to Leu substitution at site 197 in the ALS inhibitor–resistant plants, and there was a greater than 50-fold increase in EPSPS copy number in the glyphosate-resistant plants. There were no nucleotide changes in the psbA gene; therefore, atrazine resistance in this population was not target-site mediated. The evolution of this multiple herbicide-resistant Palmer amaranth population poses significant management challenges to Michigan farmers.

Keywords

AtrazineglyphosatethifensulfuronPalmer amaranth, Amaranthus palmeri S. Wats.Acetolactate synthasegene amplificationmechanism of resistancemolecular analysisresistance factorthree-way resistance

Information

Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 65 , Issue 3 , May 2017 , pp. 327 - 338
Copyright
© Weed Science Society of America, 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

Footnotes

Associate Editor for this paper: Ramon G. Leon, University of Florida.

References

Literature Cited

Anderson, MP, Gronwald, JW (1991) Atrazine resistance in a velvetleaf (Abutilon theophrasti) biotype due to enhanced glutathione S-transferase activity. Plant Physiol 96:104–109 CrossRefGoogle Scholar
Ashigh, J, Corbett, CL, Smith, PJ, Laplante, J, Tardif, FJ (2009) Characterization and diagnostic tests of resistance to acetohydroxyacid synthase inhibitors due to an Asp376Glu substitution in Amaranthus powellii . Pestic Biochem Physiol 95:38–46 CrossRefGoogle Scholar
Betha, S, Thompson, CR, Peterson, DE, Jugulam, M (2015) Mechanism(s) of resistance to PS II- and ALS-inhibitors in mesotrione-resistant Palmer amaranth. Pages 36–37 in Proceedings of 70th Annual Meeting of the North Central Weed Science Society. Indianapolis, IN: North Central Weed Science SocietyGoogle Scholar
Burgos, NR, Tranel, PJ, Streibig, JC, Davis, VM, Shaner, D, Norsworthy, JK, Ritz, C (2013) Review: conformation of resistance to herbicides and evaluation of resistance levels. Weed Sci 61:4–20 CrossRefGoogle Scholar
Chandi, A, Jordan, DL, York, AC, Milla-Lewis, SR, Burton, JD, Culpepper, AS, Whitiker, JR (2013) Interference and control of glyphosate-resistant and -susceptible Palmer amaranth (Amaranthus palmeri) populations under greenhouse conditions. Weed Sci 61:259–266 CrossRefGoogle Scholar
Culpepper, AS, Grey, TL, Vencill, WK, Kichler, JM, Webster, TM, Brown, SM, York, AC, Davis, JW, Hanna, WW (2006) Glyphosate-resistant Palmer amaranth (Amaranthus palmeri) confirmed in Georgia. Weed Sci 54:620–626 CrossRefGoogle Scholar
Devine, MD, Shukla, A (2000) Altered target sites as a mechanism of herbicide resistance. Crop Prot 19:881–889 CrossRefGoogle Scholar
Diebold, RS, McNaughton, KE, Lee, EA, Tardif, FJ (2003) Multiple resistance to imazethapyr and atrazine in Powell amaranth (Amaranthus powellii). Weed Sci 51:312–318 CrossRefGoogle Scholar
Ehleringer, J (1983) Ecophysiology of Amaranthus palmeri, a Sonoran Desert summer annual. Oecologia 57:10–112 CrossRefGoogle Scholar
Evans, CM, Tranel, P, Riechers, DE, Davis, AS, Maxwell, D, Gonzini, L, Hager, AG (2015) Characterization of a novel five-way resistant population of waterhemp Amaranthus tuberculatus. Page 61 in Proceeding of the 70th Annual Meeting of the North Central Weed Science Society. Indianapolis, IN: North Central Weed Science SocietyGoogle Scholar
Fedtke, C, Schmidt, RR (1983) Behavior of metribuzin in tolerant and susceptible soybean varieties. Pages 177–182 in Miyamoto J & Kearney PC, eds. Pesticide Chemistry: Human Welfare and the Environment Volume 3. Elmsford, NY: Pergamon Google Scholar
Foes, MJ, Liu, L, Vigue, G, Stoller, EW, Wax, LM, Tranel, PJ (1999) A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides. Weed Sci 47:20–27 CrossRefGoogle Scholar
Foes, MJ, Tranel, PJ, Wax, LM, Stoller, EW (1998) A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci, 514–520 CrossRefGoogle Scholar
Franssen, AS, Skinner, DZ, Al-Khatib, K, Horak, MJ, Kulakow, PA (2001) Interspecific hybridization and gene flow of ALS resistance in Amaranthus species. Weed Sci 49:598–606 CrossRefGoogle Scholar
Fuerst, EP, Barrett, M, Penner, D (1986) Control of triazine-resistant common lambsquarters (Chenopodium album) and two pigweed species (Amaranthus spp.) in corn (Zea mays). Weed Sci 34:440–443 CrossRefGoogle Scholar
Gaines, TA, Shaner, DL, Ward, SM, Leach, JE, Preston, C, Westra, P (2011) Mechanism of resistance of evolved glyphosate-resistant Palmer amaranth (Amaranthus palmeri). J Agric Food Chem 59:5886–5889 CrossRefGoogle ScholarPubMed
Gaines, TA, Zhang, W, Wang, D, Bukun, B, Chisholm, ST, Shaner, DL, Nissen, SJ, Patzoldt, WL, Tranel, PJ, Culpepper, AS, Grey, TL, Webster, TM, Vencill, WK, Sammons, RD, Jiang, J, Preston, C, Leach, JE, Westra, P (2010) Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc Natl Acad Sci USA 107:1029–1034 CrossRefGoogle ScholarPubMed
Ge, X, d’Avignon, DA, Ackerman, JJ, Sammons, RD (2010) Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag Sci 66:345–348 CrossRefGoogle ScholarPubMed
Giacomini, D, Westra, P, Ward, SM (2014) Impact of genetic background in fitness cost studies: an example from glyphosate-resistant Palmer amaranth. Weed Sci 62:29–37 CrossRefGoogle Scholar
Gossett, BJ, Murdock, EC, Toler, JE (1992) Resistance of Palmer amaranth (Amaranthus palmeri) to the dinitroaniline herbicides. Weed Technol 6:587–591 CrossRefGoogle Scholar
Gronwald, JW, Anderson, RN, Yee, C (1989) Atrazine resistance in velvetleaf (Abutilon theophrasti) due to enhanced atrazine detoxification. Pestic Biochem Physiol 34:149–163 CrossRefGoogle Scholar
Heap, I (2014) Herbicide resistant weeds. Pages 281–301 in Pimentel D & Peshin R, eds. Integrated Pest Management. Dordrecht, Netherlands: Springer CrossRefGoogle Scholar
Heap, I (2016) The International Survey of Herbicide Resistant Weeds. www.weedscience.com. Accessed: June 24, 2016Google Scholar
Horak, MJ, Peterson, DE (1995) Biotypes of Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) are resistant to imazethapyr and thifensulfuron. Weed Technol 9:192–195 CrossRefGoogle Scholar
Huang, Z, Chen, J, Zhang, C, Huang, H, Wei, S, Zhou, X, Chen, J, Wang, X (2016) Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.) Pestic Biochem Physiol 128:10–15 CrossRefGoogle ScholarPubMed
Jhala, AJ, Sandell, LD, Rana, N, Kruger, GR, Knezevic, SZ (2014) Confirmation and control of triazine and 4-hydroxyphenylpyruvate dioxygenase-inhibiting herbicide-resistant Palmer amaranth (Amaranthus palmeri) in Nebraska. Weed Technol 28:28–38 CrossRefGoogle Scholar
Johnson, WG, Chahal, GS, Regehr, DL (2012) Efficacy of various corn herbicides applied preplant incorporated and preemergence. Weed Technol 26:220–229 CrossRefGoogle Scholar
Koger, CH, Reddy, KN (2005) Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis). Weed Sci 55:84–89 CrossRefGoogle Scholar
Livak, KJ, Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and 2−∆∆CT method. Methods 25:402–408 CrossRefGoogle Scholar
Lorraine-Colwill, DF, Powles, SB, Hawkes, TR, Hollinshead, PH, Warner, SAJ, Preston, C (2002) Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic Biochem Physiol 74:62–72 CrossRefGoogle Scholar
Ma, R, Kaundun, SS, Tranel, PJ, Riggins, CW, McGinness, DL, Hager, AG, Hawkes, T, McIndoe, E, Riechers, DE (2013) Distinct detoxification mechanisms confer resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol 163:363–377 CrossRefGoogle Scholar
Maertens, KD, Sprague, CL, Tranel, PJ, Hines, RA (2004) Amaranthus hybridus populations resistant to atrazine and acetolactate synthase-inhibiting herbicides. Weed Res 44:21–26 CrossRefGoogle Scholar
McNaughton, KE, Letarte, J, Lee, EA, Tardif, FJ (2005) Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci 53:17–22 CrossRefGoogle Scholar
Mengistu, LW, Christoffers, MJ, Lym, RG (2005) A psbA in Kochia scoparia (L) Schrad from railroad rights-of-way with resistance to diuron, tebuthiuron and metribuzin. Pest Manag Sci 61:1035–1042 CrossRefGoogle ScholarPubMed
Mengistu, LW, Mueller-Warrant, GW, Liston, A, Barker, RE (2000) psbA mutation (valine219 to isoleucine) in Poa annua resistant to metribuzin and diuron. Pest Manag Sci 56:209–217 3.0.CO;2-8>CrossRefGoogle Scholar
Mohseni-Moghadam, M, Schroeder, J, Ashigh, J (2013) Mechanism of resistance and inheritance in glyphosate resistant Palmer amaranth (Amaranthus palmeri) populations from New Mexico, USA. Weed Sci 61:517–525 CrossRefGoogle Scholar
Nandula, VK, Reddy, KN, Koger, CH, Poston, DH, Rimando, AM, Duke, SO, Bond, JA, Ribeiro, DN (2012) Multiple resistance to glyphosate and pyrithiobac in Palmer amaranth (Amaranthus palmeri) from Mississippi and response to flumiclorac. Weed Sci 60:179–188 CrossRefGoogle Scholar
Norsworthy, JK, Griffith, GM, Scott, RC, Smith, KL, Oliver, LR (2008) Confirmation and control of glyphosate-resistant Palmer amaranth (Amaranthus palmeri) in Arkansas. Weed Technol 22:108–113 CrossRefGoogle Scholar
Owen, MDK (2008) Weed species shifts in glyphosate‐resistant crops. Pest Manag Sci 64:377–387 CrossRefGoogle ScholarPubMed
Patzoldt, WL, Dixon, BS, Tranel, PJ (2003) Triazine resistance in Amaranthus tuberculatus (Moq) Sauer that is not site-of-action mediated. Pest Manag Sci 59:1134–1142 CrossRefGoogle Scholar
Patzoldt, WL, Tranel, PJ (2007) Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Sci 55:421–428 CrossRefGoogle Scholar
Perez-Jones, A, Park, K-W, Polge, N, Colquhoun, , Mallory-Smith, CA (2007) Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta 226:395–404 CrossRefGoogle ScholarPubMed
Powles, SB, Preston, C (2006) Evolved glyphosate resistance in plants: biochemical and genetic basis of resistance. Weed Technol 20:282–289 CrossRefGoogle Scholar
Ribeiro, DN (2013) Mechanisms and Variability of Glyphosate Resistance in Amaranthus palmeri and Ipomoea lacunose Ph.D dissertation. Starkville, MS: Mississippi State University. 127 pGoogle Scholar
Ryan, GF (1970) Resistance of common groundsel to simazine and atrazine. Weed Sci 18:614–616 CrossRefGoogle Scholar
Salas, RA, Burgos, NR, Tranel, PJ, Singh, S, Glasgow, L, Scott, RC, Nichols, RL (2016) Resistance to PPO-inhibiting herbicide Palmer amaranth from Arkansas. Pest Manag Sci 72:864–869 CrossRefGoogle ScholarPubMed
Salas, RA, Dayan, FE, Pan, Z, Watson, SB, Dickson, JW, Scott, RC, Burgos, NR (2012) EPSPS gene amplification in glyphosate-resistant Italian ryegrass (Lolium perenne spp. multiflorum) from Arkansas. Pest Manag Sci 68:1223–1230 CrossRefGoogle Scholar
Salas, RA, Scott, RC, Dayan, FE, Burgos, NR (2015) EPSPS gene amplification in glyphosate-resistant Italian ryegrass (Lolium perenne spp. multiflorum) populations from Arkansas (United States). J Agric Food Chem 63:5885–5893 CrossRefGoogle Scholar
Sauer, J (1957) Recent migration and evolution of the dioecious amaranths. Evolution 11:11–31 CrossRefGoogle Scholar
Sellers, BA, Smeda, RJ, Johnson, WG, Kendig, JA, Ellersieck, MR (2003) Comparative growth of six Amaranthus species in Missouri. Weed Sci 51:329–333 CrossRefGoogle Scholar
Sibony, M, Michel, A, Haas, HU, Rubin, B, Hurle, K (2001) Sulfometuron-resistant Amaranthus retroflexus: cross-resistance and molecular basis for resistance to acetolactate synthase-inhibiting herbicides. Weed Res 41:509–522 CrossRefGoogle Scholar
Sibony, M, Rubin, B (2003) The ecological fitness of Amaranthus retroflexus and A. blitoides resistant to acetolactate synthase (ALS) inhibitors and atrazine. Weed Res 43:40–47 CrossRefGoogle Scholar
Shaner, DL (2000) The impact of glyphosate-tolerant crops on the use of other herbicides and on resistance management. Pest Manag Sci 56:320–326 3.0.CO;2-B>CrossRefGoogle Scholar
Soltani, N, Vyn, JD, Swanton, CJ, Weaver, S, Sikkema, PH (2008) Growth and fitness of triazine-susceptible and triazine-resistant common waterhemp (Amaranthus tuberculatus var. rudis). Weed Biol Manag 8:73–77 CrossRefGoogle Scholar
Sosnoskie, LM, Kichler, JM, Wallace, RD, Culpepper, AS (2011) Multiple resistance in Palmer amaranth to glyphosate and pyrithiobac confirmed in Georgia. Weed Sci 59:321–325 CrossRefGoogle Scholar
Sprague, CL (2011) Glyphosate-resistant Palmer amaranth confirmed in Michigan. MSU Weed Science. http://msuweeds.com/newsletter-holder/emerging-weed-issues/glyphosate-resistant-palmer-amaranth-confirmed-in-southwest-michigan. Accessed: July 11, 2016Google Scholar
Sprague, CL, Stoller, EW, Wax, LM, Horak, MJ (1997) Palmer amaranth (Amaranthus palmeri) and common waterhemp (Amaranthus rudis) resistance to selected ALS-inhibiting herbicides. Weed Sci 45:192–197 CrossRefGoogle Scholar
Steckel, LE, Main, CL, Ellis, AT, Mueller, TC (2008) Palmer amaranth (Amaranthus palmeri) in Tennessee has low level glyphosate resistance. Weed Technol 22:119–123 CrossRefGoogle Scholar
Tranel, PJ, Wright, TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712 CrossRefGoogle Scholar
Vencill, WK, Nichols, RL, Webster, TM, Soteres, JK, Mallory-Smith, C, Burgos, NR, Johnson, WG, McClelland, MR (2012) Herbicide resistance: toward an understanding of resistance development and the impact of herbicide-resistant crops. Weed Sci 60:2–30 CrossRefGoogle Scholar
Vogel, C, Marcotte, EM (2012) Insights into the regulation of protein abundance from proteomic and transcriptome analyses. Nat Rev Genet 13:227–232 CrossRefGoogle Scholar
Wakelin, AM, Preston, C (2006) A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res 46:432–440 CrossRefGoogle Scholar
Webster, TM, Coble, HD (1997) Changes in the weed species composition of the Southern United States: 1974 to 1995. Weed Technol 11:308–317 CrossRefGoogle Scholar
Webster, TM, Nichols, RL (2012) Changes in the prevalence of weed species in the major agronomic crops of the Southern United States: 1994/1995 to 2008/2009. Weed Sci 60:145–157 CrossRefGoogle Scholar
Whaley, CM, Wilson, HP, Westwood, JH (2007) A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides. Weed Sci 55:83–90 CrossRefGoogle Scholar
Wiersma, AT, Gaines, TA, Preston, C, Hamilton, JP, Giacomini, D, Buell, CR, Leach, JE, Westra, P (2015) Gene amplification of 5-enol-pyruvylshikimate-3-phosphate synthase in glyphosate-resistant Kochia scoparia . Planta 241:463–474 CrossRefGoogle ScholarPubMed
Wiggins, MS, McClure, MA, Hayes, RM, Steckel, LE (2015) Integrating cover crops and POST herbicides for glyphosate-resistant Palmer amaranth control in corn. Weed Technol 29:412–418 CrossRefGoogle Scholar
Yaacoby, T, Schonfeld, M, Rubin, B (1986) Characteristics of atrazine-resistant biotypes of three grass weeds. Weed Sci 34:181–184 CrossRefGoogle Scholar
Young, BG (2006) Changes in herbicide use patterns and production practices resulting from glyphosate-resistant crops 1. Weed Technol 20:301–307 CrossRefGoogle Scholar