Hostname: page-component-848d4c4894-5nwft Total loading time: 0 Render date: 2024-06-07T05:45:07.913Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of Isocitric Lyase Activity in Germinating Prickly Sida Seed

Published online by Cambridge University Press:  12 June 2017

Bonnie J. Reger  and
E. Wayne Smith
Bonnie J. Reger
Affiliation:
S. Weed Sci. Lab., Agr. Res. Serv., U.S. Dep. Agr., Stoneville, MS 38776
E. Wayne Smith
Affiliation:
S. Weed Sci. Lab.
Get access Rights & Permissions [Opens in a new window]

Abstract

Maximum activity of isocitric lyase (EC 4.1.3.1) was reached at 24 hr incubation of afterripened (nondormant) prickly sida (Sida spinosa L.) seed. Enzyme activity declined gradually with incubation times in excess of 24 hr. Actinomycin D (Act D) at 5 μg/ml had essentially no effect on 24-hr germination but inhibited development of isocitric lyase activity 83%. Cycloheximide (CH) at 10 μg/ml inhibited 24-hr germination of punctured seed only 7% but inhibited development of isocitric lyase activity 76%. Seed incubated in water 12 hr before being transferred to Act D at 5 μg/ml for an additional 12 hr did not escape sensitivity to the antibiotic. Isocitric lyase activity was inhibited when assayed at 24 hr total incubation. In the reverse experiment, seed incubated in Act D 12 hr before being transferred to water, isocitric lyase activity at 24 hr was not affected. Apparently prickly sida seed lacked a performed mRNA for isocitric lyase and transcription and translation of the mRNA occurred shortly after initiation of radicle protrusion (~8 hr).

Type
Research Article
Information
Weed Science , Volume 24 , Issue 3 , May 1976 , pp. 298 - 301
Copyright
Copyright © 1976 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Beevers, H. 1969. Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann. N.Y. Acad. Sci. 168:313–324.Google Scholar
2. Breidenbach, W. and Beevers, H. 1967. Association of the glyoxylate enzymes in a novel subcellular particle from castor bean endosperm. Biochem. Biophys. Res. Commun. 27:462–469.CrossRefGoogle Scholar
3. Carpenter, W.D. and Beevers, H. 1959. Distribution and properties of isocitritase in plants. Plant Physiol. 34:403–409.Google Scholar
4. Dixon, G.H. and Kornberg, H.L. 1959. Assay methods for key enzymes of the glyoxylate cycle. Biochem. J. 72:3P.Google Scholar
5. Dure, L. and Waters, L. 1965. Long-lived messenger RNA: evidence from cotton seed germination. Science 147:410–412.Google Scholar
6. Egley, G.H. 1976. Germination of developing prickly sida seed. Weed Sci. 24:In Press.Google Scholar
7. Gientka-Rychter, A. and Cherry, J.H. 1968. De novo synthesis of isocitritase in peanut (Arachis hypogaea L.) cotyledons. Plant Physiol. 43:653–659.Google Scholar
8. Hock, B. and Beevers, H. 1966. Development and decline of glyoxylate-cycle enzymes in watermelon seedlings (Citrullus vulgaris Schrad)–effects of dactinomycin and cycloheximide. Z. Pflanzenphysiol. 55:405–414.Google Scholar
9. Ihle, J.N. and Dure, L. III. 1969. Synthesis of a protease in germinating cotton cotyledons catalyzed by mRNA synthesized during embryogenesis. Biochem. Biophys. Res. Commun. 36:705–710.CrossRefGoogle Scholar
10 Ihle, J.N. and Dure, L. III. 1972. The developmental biochemistry of cottonseed embryogenesis and germination. J. Biol. Chem. 247:5048–5055.Google Scholar
11. Kagawa, T., McGregor, D.I., and Beevers, H. 1973. Development of enzymes in the cotyledons of watermelon seedlings. Plant Physiol. 51:66–71.Google Scholar
12. Lam, T.H. and Shaw, M. 1970. Removal of phenolics from plant extracts by grinding with anion exchange resin. Biochem. Biophys. Res. Commun. 39:965–968.Google Scholar
13. Longo, C.P. 1968. Evidence for de novo synthesis of isocitritase and malate synthetase in germinating peanut cotyledons. Plant Physiol. 43:660–664.CrossRefGoogle Scholar
14. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.Google Scholar
15. Schnarrenberger, C., Oeser, A., and Tolbert, N.E. 1971. Development of microbodies in sunflower and castor bean endosperm during germination. Plant Physiol. 48:566–574.CrossRefGoogle ScholarPubMed
16. Smith, R.H., Schubert, A.M., and Benedict, C.R. 1974. The development of isocitric lyase activity in germinating cottonseed. Plant Physiol. 54:197–200.CrossRefGoogle Scholar
17. Tolbert, N.E. 1971. Microbodies–peroxisomes and glyoxysomes. Ann. Rev. Plant Physiol. 22:45–74.Google Scholar