Hostname: page-component-8448b6f56d-xtgtn Total loading time: 0 Render date: 2024-04-23T06:41:37.944Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution of Herbicide Resistances and Molecular Mechanisms Conferring Resistance in Missouri Waterhemp (Amaranthus rudis Sauer) Populations

Published online by Cambridge University Press:  20 January 2017

John L. Schultz ,
Laura A. Chatham ,
Chance W. Riggins ,
Patrick J. Tranel  and
Kevin W. Bradley
John L. Schultz
Affiliation:
Division of Plant Sciences, 201 Waters Hall, University of Missouri, Columbia, MO 65211
Laura A. Chatham
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Chance W. Riggins
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Patrick J. Tranel
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Kevin W. Bradley*
Affiliation:
Division of Plant Sciences, 201 Waters Hall, University of Missouri, Columbia, MO 65211
*
Corresponding author's E-mail: bradleyke@missouri.edu
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A survey of soybean fields containing waterhemp was conducted just prior to harvest in 2012 to determine the scope and extent of herbicide resistance and multiple herbicide resistances among a sample of Missouri waterhemp populations. Resistance was confirmed to glyphosate and to acetolactate synthase (ALS), protoporphyrinogen oxidase (PPO), photosystem II (PSII), and 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors, but not to 2,4-D. Of the 187 populations tested, 186 exhibited resistance to chlorimuron. The proportions of populations with atrazine or glyphosate resistance were similar, with 30 and 29% of the populations surviving the 3× rates. Lactofen resistance was observed in 5% of the populations, whereas mesotrione resistance was only found in 1.6% of the populations. All populations tested were susceptible to 2,4-D at the 3× rate. At least 52% of the waterhemp populations tested exhibited resistance to herbicides from two mechanism of action. Resistance to atrazine plus chlorimuron as well as glyphosate plus chlorimuron was present in 29% of the populations. Three-way resistance, primarily comprised of resistance to atrazine plus chlorimuron plus glyphosate, was present in 11% of the populations. Resistance to herbicides from four mechanisms of action was found in 2% of the populations, and one population exhibited resistance to herbicides from five mechanisms of action. DNA analysis of a subsample of plants revealed that previously documented mechanisms of resistance in waterhemp, including the ΔG210 deletion conferring PPO-inhibitor resistance, the Trp574Leu amino acid substitution conferring ALS-inhibitor resistance, and elevated 5-enolypyruvyl-shikimate-3-phosphate synthase copy number and the Pro106Ser amino acid substitution resulting in glyphosate resistance, explained survival in many, but not all, instances. Atrazine resistance was not explained by the Ser264Gly D1 protein substitution. Overall, results from these experiments indicate that Missouri soybean fields contain waterhemp populations with resistance to glyphosate, ALS-, PPO-, PSII-, and HPPD-inhibiting herbicides, which are some of the most common mechanisms of action currently utilized for the control of this species in corn and soybean production systems. Additionally, these results indicate that slightly more than half of the populations tested exhibit resistance to more than one herbicide mechanisms of action. Managing the current resistance levels in existing populations is of utmost importance. The use of multiple, effective herbicide modes of action, both preemergence and postemergence, and the integration of optimum cultural and mechanical control practices will be vital in the management of Missouri waterhemp populations in the future.

Keywords

2,4-Datrazinechlorimuronglyphosatelactofenmesotrionewaterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer)corn, Zea mays L.soybean, Glycine max (L.) Merr.Herbicide resistancesurvey
Type
Weed Management
Information
Weed Science , Volume 63 , Issue 1 , March 2015 , pp. 336 - 345
Copyright
Copyright © Weed Science Society of America 

References

Literature Cited

Beckie, HJ, Heap, IM, Smeda, RJ, Hall, LM (2000) Screening for herbicide resistance in weeds. Weed Technol 14:428–445 Google Scholar
Bell, MS, Hager, AG, Tranel, PJ (2013) Multiple resistance to herbicides from four site-of-action groups in waterhemp (Amaranthus tuberculatus). Weed Sci. 61:460–468 Google Scholar
Bradley, KW (2013) Herbicide-resistance in the Midwest: current status and impacts. Weed Sci Soc Am Abstr 271 Google Scholar
Bradley, KW, Legleiter, T, Hunter, L, Nichols, C, Foresman, C (2007) The status of glyphosate-resistant waterhemp in Missouri. Champaign, IL North Central Weed Sci Soc Abstr 192Google Scholar
Chatham, LA, Riggins, CW, Martin, JR, Kruger, GR, Bradley, KW, Peterson, DE, Jugulam, M, Tranel, P (2013) A multi-state study of the association between glyphosate resistance and EPSPS amplification in waterhemp. Champaign, IL North Central Weed Sci Soc Abstr 127Google Scholar
Cordes, JC, Johnson, WG, Scharf, P, Smeda, RJ (2004) Late-emerging common waterhemp (Amaranthus rudis) interference in conventional tillage corn. Weed Technol 18:999–1005 Google Scholar
Costea, M, Weaver, SE, Tardif, FJ (2005) The biology of invasive alien plants in Canada. 3. Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif. Can J Plant Sci. 85:507–522 Google Scholar
Délye, C, Duhoux, A, Pernin, F, Riggins, C, Tranel, P (2014) Molecular mechanisms of herbicide resistance. Weed Sci. 63:91–115 Google Scholar
Doyle, JJ, Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15 Google Scholar
Foes, MJ, Liu, L, Bigue, G, Stoller, EW, Wax, LM, Tranel, PJ (1999) A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides. Weed Sci. 47:20–27 Google Scholar
Foes, MJ, Liu, L, Tranel, PJ, Wax, LM, Stoller, EW (1998) A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci. 46:514–520 Google Scholar
Givens, WA, Shaw, DR, Johnson, WG, Weller, SC, Young, BG, Wilson, RG, Owen, MDK, Jordan, D (2009) A grower survey of herbicide use patterns in glyphosate-resistant cropping systems. Weed Technol 23:156–161 Google Scholar
Guo, J, Riggins, CW, Hausman, N, Hager, AG, Riechers, DE, Tranel, P (2013) Non–target-site resistance to ALS inhibitors in waterhemp. Champaign, IL North Central Weed Sci Soc Abstr 128Google Scholar
Hager, A, Wax, L, Simmons, W, Sprague, C (2000) Waterhemp management in Illinois agronomic crops. 2000 Illinois Agricultural Pest Management Handbook. Champaign, IL University of Illinois Extension. Pp 91–100 Google Scholar
Hager, AG, Wax, LM, Stoller, EW, Bollero, GA (2002) Common waterhemp (Amaranthus rudis) interference in soybean. Weed Sci. 50:607–610 Google Scholar
Hartzler, RG, Battles, BA, Nordby, D (2004) Effect of common waterhemp (Amaranthus rudis) emergence date on growth and fecundity in soybean. Weed Sci. 52:242–245 Google Scholar
Hartzler, RG, Buhler, DD, Stoltenberg, DE (1999) Emergence characteristics of four annual weed species. Weed Sci. 47:578–584 Google Scholar
Hausman, NE, Singh, S, Tranel, PJ, Riechers, DE, Kaundun, SS, Polge, ND, Thomas, DA, Hager, AG (2011) Resistance to HPPD-inhibiting herbicides in a population of waterhemp (Amaranthus tuberculatus) from Illinois, United States. Pest Manag Sci. 67:253–261 Google Scholar
Heap, I (2014) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org/summary/home.aspx. Accessed February 2, 2014Google Scholar
Johnson, WG, Gibson, KD (2006) Glyphosate-resistant weeds and resistance management strategies: an Indiana grower perspective. Weed Technol 20:768–772 Google Scholar
Lee, RM, Hager, AG, Tranel, PJ (2008) Prevalence of a novel resistance mechanism to PPO-inhibiting herbicides in waterhemp (Amaranthus tuberculatus). Weed Sci. 56:371–375 Google Scholar
Legleiter, TR, Bradley, KW (2008) Glyphosate and multiple herbicide resistance in common waterhemp (Amaranthus rudis) populations from Missouri. Weed Sci. 56:582–587 Google Scholar
Ma, R, Kaundun, SS, Tranel, PJ, Riggins, CW, McGinness, DL, Hager, AG, Hawkes, T, McIndoe, E, Riechers, DE (2013) Distinct detoxification mechanisms confer resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol 163:363–377 Google Scholar
Mechant, E, De Marez, T, Hermann, O, Bulcke, R (2008) Resistance of Chenopodium album to photosystem II-inhibitors. Commun Agric Appl Biol Sci. 73:913–917 Google Scholar
Nandula, VK, Ray, JD, Ribeiro, DN, Pan, RZ, Reddy, KN (2013) Glyphosate resistance in tall waterhemp (Amaranthus tuberculatus) from Mississippi is due to both altered target-site and nontarget-site mechanisms. Weed Sci. 61:374–383 Google Scholar
Patzoldt, WL, Dixon, BS, Tranel, PJ (2003) Triazine resistance in Amaranthus tuberculatus (Moq) Sauer that is not site-of-action mediated. Pest Manag Sci. 59:1134–1142 Google Scholar
Patzoldt, WL, Hager, AG, McCormick, Tranel PJ (2006) A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase. Proc Natl Acad Sci U S A 103:12329–12334 Google Scholar
Patzoldt, WL, Tranel, PJ (2007) Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Sci. 55:421–428 Google Scholar
Patzoldt, WL, Tranel, PJ, Hager, AG (2002) Variable herbicide responses among Illinois waterhemp (Amaranthus rudis and A. tuberculatus) populations. Crop Prot 21:707–712 Google Scholar
Patzoldt, WL, Tranel, PJ, Hager, AG (2005) A waterhemp (Amaranthus tuberculatus) biotype with multiple resistance across three herbicide sites of action. Weed Sci. 53:30–36 Google Scholar
Powles, SB (2008) Evolved glyphosate-resistant weeds around the world: lessons to be learnt. Pest Manag Sci. 64:360–365 Google Scholar
Powles, SB (2010) Gene amplification delivers glyphosate-resistant weed evolution. Proc Natl Acad Sci U S A 107:955–956 Google Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Ann Rev Plant Biol. 64:360–365 Google Scholar
Rosenbaum, KK, Bradley, KW (2013) A survey of glyphosate-resistant waterhemp in Missouri soybean fields and prediction of glyphosate resistance in future waterhemp populations based on in-field observations and management practices. Weed Technol 27:656–663 Google Scholar
Sauer, J (1957) Recent migration and evolution of the dioecious amaranths. Evolution 11:11–31 Google Scholar
Shoup, DE, Al-Khatib, K, Peterson, DE (2003) Common waterhemp (Amaranthus rudis) resistance to protoporphyrinogen oxidase–inhibiting herbicides. Weed Sci. 51:145–150 Google Scholar
Steckel, LE (2007) The dioecious Amaranthus spp.: here to stay. Weed Technol 21:567–570 Google Scholar
Tamura, K, Stecher, G, Peterson, D, Filipski, A, Kumar, S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729 Google Scholar
Teaster, ND, Hoagland, RE (2014) Characterization of glyphosate resistance in cloned Amaranthus palmeri plants. Weed Biol Manag. 14:1–10 Google Scholar
Thinglum, KA, Riggins, CW, Davis, AS, Bradley, KW, Al-Khatib, K, Tranel, PJ (2011) Wide distribution of the waterhemp (Amaranthus tuberculatus) ΔG210 PPX2 mutation, which confers resistance to PPO-inhibiting herbicides. Weed Sci. 59:22–27 Google Scholar
Tranel, PJ, Riggins, CW, Bell, MS, Hager, AG (2011) Herbicide resistance in Amaranthus tuberculatus: a call for new options. J Agric Food Chem. 59:5808–5812 Google Scholar
Waggoner, BS, Bradley, KW (2011) A survey of weed incidence and severity in response to management practices in Missouri soybean production fields. Champaign, IL North Central Weed Sci Soc Abstr 80Google Scholar
Warwick, SI, Sauder, CA, Beckie, HJ (2010) Acetolactate synthase (ALS) target-site mutations in ALS inhibitor-resistant Russian thistle (Salsola tragus). Weed Sci. 58:244–251 Google Scholar
Young, BG (2006) Changes in herbicide use patterns and production practices resulting from glyphosate resistant crops. Weed Technol 20:301–307 Google Scholar