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Influence of Simazine and Atrazine on Free Fatty Acid Content in Isolated Chloroplasts

Published online by Cambridge University Press:  12 June 2017

A. E. Smith  and
R. E. Wilkinson
A. E. Smith
Affiliation:
Agron. Univ. of Ga. College of Agr. Exp. Sta., Ga. Sta., Experiment, Georgia 30212
R. E. Wilkinson
Affiliation:
Agron. Univ. of Ga. College of Agr. Exp. Sta., Ga. Sta., Experiment, Georgia 30212
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Abstract

Chloroplasts, isolated from fresh spinach (Spinacia oleracea L.) were treated with four concentrations of each 2-chloro-4,6-bis(ethylamino)-s-triazine (simazine) or 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine) to determine the influence of these herbicides on the free fatty acid content in isolated chloroplasts. The treated chloroplast solutions were sampled at 0, 5, 15, 30, 60, and 120 min following treatment. Results indicated that most herbicide treatments increased free fatty acid content in isolated chloroplasts when compared with the zero concentration treatments. The increased fatty acid content was similar for most concentrations of simazine and atrazine. Results of individual fatty acid analyses indicated that the increase in total fatty acids was a result of increases in concentration of unsaturated rather than saturated fatty acids.

Type
Research Article
Information
Weed Science , Volume 21 , Issue 1 , January 1973 , pp. 57 - 60
Copyright
Copyright © 1973 Weed Science Society of America 

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References

Literature Cited

1. Arnon, D. E. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris . Plant Physiol. 24:1–15.Google Scholar
2. Ashton, F. M. 1965. Physiological, biochemical, and structural modifications of plants induced by atrazine and monuron. Proc. S. Weed Conf. 18:596–602.Google Scholar
3. Ashton, F. M., Zweig, G., and Mason, G. W. 1960. The effect of certain triazines on C14O2 fixation in red kidney beans. Weeds 8:448–451.Google Scholar
4. Ashton, F. M., Uribe, E. G., and Zweig, G. 1961. Effect of monuron on C14O2 fixation in red kidney bean leaves. Weeds 9:575–579.Google Scholar
5. Ashton, F. M., Gifford, E. M. Jr., and Bisalputra, T. 1963. Structural changes in Phaseolus vulgaris induced by atrazine. I. Histological changes. Bot. Gaz. 124:329–335.Google Scholar
6. Ashton, F. M., Gifford, E. M. Jr., and Bisalputra, T. 1963. Structural changes in Phaseolus vulgaris induced by atrazine. II. Effects on fine structure of chloroplasts. Bot. Gaz. 124:336–343.Google Scholar
7. Chen, T. M., Brown, R. H., and Black, C. C. Jr. 1969. Photosynthetic activity of chloroplasts isolated from Bermudagrass (Cynodon dactylon L.), a species with a high photosynthetic capacity. Plant Physiol. 44:649–654.Google Scholar
8. Constantopoulos, G. and Kenyon, C. N. 1968. Release of free fatty acids and loss of Hill activity by ageing spinach chloroplasts. Plant Physiol. 43:531–536.CrossRefGoogle Scholar
9. Cooke, A. R. 1956. A possible mechanism of action of the urea type herbicides. Weeds 4:397–398.Google Scholar
10. Jagendorf, A. T. 1958. The relationship between electron transport and phosphorylation in spinach chloroplasts. Brookhaven Symp. Biol. 11:236–257.Google Scholar
11. Jellum, M. D. and Worthington, R. E. 1967. Rapid gas-liquid chromatographic procedure for the analysis of methyl esters of long chain fatty acids. J. Amer. Oil Chem. Soc. 43:661–664.Google Scholar
12. Krogman, D. W. and Jagendorf, A. T. 1959. Inhibition of the Hill reaction by fatty acids and metal chelating agents. Arch. Biochem. Biophys. 80:421–430.Google Scholar
13. Malotkovsky, Y. G. and Zheskova, I. M. 1965. The influence of heating on the morphology and photochemical activity of isolated chloroplasts. Biochem. Biophys. Res. Commun. 20:411–415.Google Scholar
14. Moreland, D. E., Gentner, W. A., Hill, K. L., and Hilton, J. L. 1959. Studies on the mechanism of herbicidal action of 2-chloro-4,6-bis(ethylamino)-s-triazine. Plant Physiol. 34:432–435.Google Scholar
15. McCarty, R. E. and Jagendorf, A. T. 1965. Chloroplast damage due to enzymatic hydrolysis of endogenous lipids. Plant Physiol. 40:725–735.Google Scholar
16. Sastry, P. S. and Kates, M. 1963. Lipid components of leaves III. Isolation and characterization of mono- and digalactosyl digylcerides and lecithin. Biochem. Biophys. Acta. 70:214–216.Google Scholar
17. Sastry, P. S. and Kates, M. 1964. Hydrolysis of monogalactosyl and digalactosyl diglycerides by specific enzymes in runner-bean leaves. Biochemistry 3:1280–1287.Google Scholar
18. Steel, R. G. D. and Torrie, J. H. 1960. Principles and procedures of statistics. McGraw-Hill Book Co., New York, N. Y. 481 p.Google Scholar
19. Sweetser, P. B. and Todd, C. W. 1961. The effect of monuron and oxygen liberation in photosynthesis. Biochem. Biophys. Acta. 51:504–508.Google Scholar
20. Wessels, J. S. C. and Van der Veen, R. 1956. The action of some derivatives of phenylurethan and 3-phenyl-1-dimethylurea on the Hill reaction. Biochem. Biophys. Acta. 19:548–549.CrossRefGoogle ScholarPubMed
21. Zweig, G. and Ashton, F. M. 1962. The effect of 2-chloro-4-ethylamino-6-isopropylamino-s-triazine (atrazine) in distribution of C14O2 fixation in excised kidney bean leaves. J. Exp. Bot. 13:5–11.Google Scholar