Hostname: page-component-848d4c4894-wzw2p Total loading time: 0 Render date: 2024-05-14T06:45:01.192Z Has data issue: false hasContentIssue false
  • English
  • Français

Inhibition of Chlorophyll Accumulation by Glyphosate

Published online by Cambridge University Press:  12 June 2017

Lynn M. Kitchen ,
William W. Witt  and
Charles E. Rieck
Lynn M. Kitchen
Affiliation:
Agron. Dep., Univ. of Kentucky, Lexington, KY 40546
William W. Witt
Affiliation:
Agron. Dep., Univ. of Kentucky, Lexington, KY 40546
Charles E. Rieck
Affiliation:
Agron. Dep., Univ. of Kentucky, Lexington, KY 40546
Get access Rights & Permissions [Opens in a new window]

Abstract

Glyphosate [N-(phosphonomethyl)glycine] significantly decreased the chlorophyll content of field-grown soybeans (Glycine max L. Merr. ‘Williams’) within 48 h after a 2.24-kg/ha treatment. In laboratory studies, 0.0001, 0.001, 0.01, 0.1, and 1 mM glyphosate reduced chlorophyll content of 7-day-old etiolated barley (Hordeum vulgare L. ‘Barsoy’) shoots 8, 12, 25, 49, and 77%, respectively, following 8 h dark incubation and 24 h illumination. Reduction of chlorophyll accumulation ranged from 6% with 0.001 mM glyphosate to 82% with 1.0 mM glyphosate. In studies with 8-day-old etiolated corn (Zea mays L. ‘Pioneer Brand 3535’) shoots, 0.1, 1.0, and 10.0 mM glyphosate decreased chlorophyll content of corn shoots 24, 42, and 50%, respectively, after 12 h of illumination. The rate of chlorophyll accumulation in corn shoots was significantly reduced 64% by 1.0 mM glyphosate over a 15-h illumination period. These rapid and substantial effects on chlorophyll accumulation suggest that interference with greening may be important in the mechanism of action of glyphosate.

Keywords

Mode-of-actionchlorosisillumination
Type
Research Article
Information
Weed Science , Volume 29 , Issue 4 , July 1981 , pp. 513 - 516
Copyright
Copyright © 1981 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Abu-Irmaileh, B. E. and Jordan, L. S. 1978. Some aspects of glyphosate action in purple nutsedge (Cyperus rotundus . Weed Sci. 26:700–703.CrossRefGoogle Scholar
2. Amrhein, N., Deus, Brigette, Gehrke, P., and Steinrücken, H. C. 1980. The site of the inhibition of the shikimate pathway by glyphosate. II. Interference of glyphosate with chorismate formation in vivo and in vitro. Plant Physiol. 66:830–834.CrossRefGoogle ScholarPubMed
3. Beale, S. I. and Castelfranco, P. A. 1974. The biosynthesis of δ-aminolevulinic acid in higher plants. I. Accumulation of δ-aminolevulinic acid in greening plant tissues. Plant Physiol. 53: 291–296.CrossRefGoogle ScholarPubMed
4. Bogorad, L. 1966. The biosynthesis of chlorophylls. Pages 481–510 in Vernon, L. P. and Seely, G. R., eds. The Chlorophylls. Academic Press, New York.CrossRefGoogle Scholar
5. Campbell, W. F., Evans, S. O., and Reed, S. C. 1976. Effects of glyphosate on chlorophyll ultrastructure of quackgrass mesophyll cells. Weed Sci. 24:22–25.CrossRefGoogle Scholar
6. Duke, S. O. and Hoagland, R. E. 1979. Glyphosate and light effects on the induction of phenylalanine ammonia-lyase activity in cotton and soybean seedlings. Abstr., Weed Sci. Soc. Am. p. 96.Google Scholar
7. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1979. Effects of glyphosate on metabolism of phenolic compounds. IV. Phenylalanine ammonia-lyase activity, free amino acids, and soluble hydroxy-phenolic compounds in axes of light-grown soybeans. Physiol. Plant 46:307–317.CrossRefGoogle Scholar
8. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1980. Effects of glyphosate on metabolism of phenolic compounds. V. L-α-aminooxy-β-phenylpropionic acid and glyphosate effects on phenylalanine ammonia-lyase in soybean seedlings. Plant Physiol. 65:17–21.CrossRefGoogle ScholarPubMed
9. Fernandez, C. H. and Bayer, D. E. 1977. Penetration, translocation, and toxicity of glyphosate in bermudagrass (Cynodon dactylon . Weed Sci. 25:396–400.CrossRefGoogle Scholar
10. Hoagland, R. E. 1980. Effects of glyphosate on metabolism of phenolic compounds: VI. Effects of glyphosine and glyphosate metabolites on phenylalanine ammonia-lyase activity, growth, and protein, chlorophyll, and anthocyanin levels in soybean (Glycine max) seedlings. Weed Sci. 28:393–399.CrossRefGoogle Scholar
11. Hoagland, R. E., Duke, S. O., and Elmore, D. C. 1979. Effects of glyphosate on metabolism of phenolic compounds. III. Phenylalanine ammonia-lyase activity, free amino acids, soluble protein and hydroxyphenolic compounds in axes of dark-grown soybeans. Physiol. Plant 46:357–366.CrossRefGoogle Scholar
12. Hoagland, R. E. and Paul, R. 1978. Ultrastructural effects of glyphosate on Lemma gibba L. Abstr., Weed Sci. Soc. Am. p. 78.Google Scholar
13. Hollander, Heike and Amrhein, N. 1980. The site of the inhibition of the shikimate pathway by glyphosate. I. Inhibition by glyphosate of phenylpropanoid synthesis in buckwheat (Fagopyrum esculentum Moench). Plant Physiol. 66:823–829.CrossRefGoogle ScholarPubMed
14. Hull, H. M., Bleckmann, C. A., and Morton, H. L. 1977. Some effects of glyphosate on the foliar ultrastructure of velvet mesquite seedlings. Proc. West. Weed Sci. Soc. 30:18.Google Scholar
15. Jaworski, E. G. 1972. Mode of action of N-phosphonomethyl glycine: inhibition of aromatic amino acid synthesis. J. Agric. Food Chem. 20:1195–1198.CrossRefGoogle Scholar
16. Parochetti, J. V., Wilson, H. P., and Burt, G. W. 1975. Activity of glyphosate on johnsongrass. Weed Sci. 23:395–400.CrossRefGoogle Scholar
17. Sestak, Z. 1971. Determination of chlorophyll a and b. Pages 672–701 in Sestak, Z., Catdky, J., and Jarvis, P. G., eds. Plant Photosynthetic Production – Manual of Methods. Dr. W. Junk N.W., Publishers.Google Scholar
18. Sprankle, P., Sandberg, C. L., Meggit, W. F., and Penner, D. 1978. Separation of glyphosate and possible metabolites by thin-layer chromatography. Weed Sci. 26:673–674.CrossRefGoogle Scholar
19. Zandstra, B. H. and Nishimoto, R. K. 1977. Movement and activity of glyphosate in purple nutsedge. Weed Sci. 25: 268–274.CrossRefGoogle Scholar