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Molecular Mechanism of Mesosulfuron-Methyl Resistance in Multiply-Resistant American Sloughgrass (Beckmannia syzigachne)

Published online by Cambridge University Press:  20 January 2017

Lingxu Li ,
Weitang Liu ,
Yucheng Chi ,
Wenlei Guo ,
Xiaoyong Luo  and
Jinxin Wang
Lingxu Li
Affiliation:
College of Agronomy and Plant Protection, 424 Chemistry Building, Qingdao Agricultural University, Shandong, Qingdao 266109, PR China
Weitang Liu
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, 305 Pesticide Building, Shandong Agricultural University, Shandong, Tai'an 271018, PR China
Yucheng Chi
Affiliation:
Shandong Peanut Research Institute, 126 Wannianquan Road, Shandong, Qingdao 266100, PR China
Wenlei Guo
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, 305 Pesticide Building, Shandong Agricultural University, Shandong, Tai'an 271018, PR China
Xiaoyong Luo
Affiliation:
College of Agronomy and Plant Protection, 424 Chemistry Building, Qingdao Agricultural University, Shandong, Qingdao 266109, PR China
Jinxin Wang*
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, 305 Pesticide Building, Shandong Agricultural University, Shandong, Tai'an 271018, PR China
*
Corresponding author's E-mail: wangjx@sdau.edu.cn
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Abstract

American sloughgrass is a troublesome grass weed in winter wheat fields after rice in China. Mesosulfuron-methyl failed to control American sloughgrass in Danyang County in 2012. The purpose of this research was to determine the resistance level to mesosulfuron and other herbicides in American sloughgrass and to identify the molecular basis of resistance. Dose–response experiments indicated that this population was moderately resistant to mesosulfuron-methyl (7.6-fold) and pyroxsulam (6.0-fold), highly resistant to flucarbazone-sodium (20.3-fold), fenoxaprop-p-ethyl (565.0-fold), clodinafop-proargyl (19.5-fold), and pinoxaden (45.9-fold), and susceptible to isoproturon. Part of the acetolactate sythase (ALS) gene was cloned and sequenced to confirm the molecular mechanism of resistance to ALS-inhibiting herbicides. A Pro197Ser substitution was identified. This substitution is likely the molecular mechanism of resistance to mesosulfuron-methyl in the Danyang population in which it is cross-resistant to flucarbazone-sodium and pyroxsulam. This study established the first report of mesosulfuron-methyl resistance likely caused by a Pro197 substitution in American sloughgrass and a potential herbicide to control this resistant weed.

Keywords

Clodinafop-propargylfenoxaprop-p-ethylflucarbazone-sodiumisoproturonmesosulfuron-methylpinoxadenpyroxsulamAmerican sloughgrass, Beckmannia syzigachne Steudmaize, Zea mays L.rice, Oryza sativa L.winter wheat, Triticum aestivum L.ALScross-resistanceresistancesubstitution
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 781 - 787
Copyright
Copyright © Weed Science Society of America 

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