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Multiple herbicide resistance in California Italian ryegrass (Lolium perenne ssp. multiflorum): characterization of ALS-inhibiting herbicide resistance

Published online by Cambridge University Press:  29 March 2019

Parsa Tehranchian Open the ORCID record for Parsa Tehranchian [Opens in a new window] ,
Vijay K. Nandula Open the ORCID record for Vijay K. Nandula [Opens in a new window] ,
Maor Matzrafi Open the ORCID record for Maor Matzrafi [Opens in a new window]  and
Marie Jasieniuk Open the ORCID record for Marie Jasieniuk [Opens in a new window]
Parsa Tehranchian*
Affiliation:
Former Postdoctoral Research Fellow, Department of Plant Sciences, University of California–Davis, Davis, CA, USA
Vijay K. Nandula
Affiliation:
Research Plant Physiologist, Crop Production Systems Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA
Maor Matzrafi
Affiliation:
Postdoctoral Research Associate, Department of Plant Sciences, University of California–Davis, Davis, CA, USA
Marie Jasieniuk
Affiliation:
Professor, Department of Plant Sciences, University of California–Davis, Davis, CA, USA
*
Author for correspondence: Parsa Tehranchian, Email: ptehranchian@syntechresearch.com
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Abstract

Multiple resistance to glyphosate, sethoxydim, and paraquat was previously confirmed in two Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot] populations, MR1 and MR2, in northern California. Preliminary greenhouse studies revealed that both populations were also resistant to imazamox and mesosulfuron, both of which are acetolactate synthase (ALS)-inhibiting herbicides. In this study, three subpopulations, MR1-A (from seed of MR1 plants that survived a 16X rate of sethoxydim), MR1-P (from seed of MR1 plants that survived a 2X rate of paraquat), and MR2 (from seed of MR2 plants that survived a 16X rate of sethoxydim), were investigated to determine the resistance level to imazamox and mesosulfuron, evaluate other herbicide options for the control of these multiple resistant L. perenne ssp. multiflorum, and characterize the underlying ALS-inhibitor resistance mechanism(s). Based on LD50 values, the MR1-A, MR1-P, and MR2 subpopulations were 38-, 29-, 8-fold and 36-, 64-, and 3-fold less sensitive to imazamox and mesosulfuron, respectively, relative to the susceptible (Sus) population. Only MR1-P and MR2 plants were cross-resistant to rimsulfuron, whereas both MR1 subpopulations were cross-resistant to imazethapyr. Pinoxaden (ACCase inhibitor [phenylpyrazoline 'DEN']) only controlled MR2 and Sus plants at the labeled field rate. However, all plants were effectively controlled (>99%) with the labeled field rate of glufosinate. Based on I50 values, MR1-A, MR-P, and MR2 plants were 712-, 1,104-, and 3-fold and 10-, 18-, and 5-fold less responsive to mesosulfuron and imazamox, respectively, than the Sus plants. Sequence alignment of the ALS gene of resistant plants revealed a missense single-nucleotide polymorphism resulting in a Trp-574-Leu substitution in MR1-A and MR1-P plants, heterozygous in both, but not in the MR2 plants. An additional homozygous substitution, Asp-376-Glu, was identified in the MR1-A plants. Addition of malathion or piperonyl butoxide did not alter the efficacy of mesosulfuron on MR2 plants. In addition, the presence of 2,4-D had no effect on the response of mesosulfuron on the MR2 and Sus. These results suggest an altered target site is the mechanism of resistance to ALS inhibitors in MR1-A and MR1-P plants, whereas a non–target site based resistance apparatus is present in the MR2 plants.

Keywords

Prashant Jha, Montana State UniversityCross-resistanceimazamoxmesosulfuronmultiple resistance mechanism
Type
Research Article
Information
Weed Science , Volume 67 , Issue 3 , May 2019 , pp. 273 - 280
Copyright
© Weed Science Society of America, 2019 

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References

Avila-Garcia, WV, Mallory-Smith, C (2011) Glyphosate-resistant Italian ryegrass (Lolium perenne) populations also exhibit resistance to glufosinate. Weed Sci 59:305–309CrossRefGoogle Scholar
Busi, R, Vila-Aiub, MM, Powles, SB (2011) Genetic control of a cytochrome P450 metabolism-based herbicide resistance mechanism in Lolium rigidum. Heredity 106:817–824CrossRefGoogle ScholarPubMed
DiTomaso, JM, Healy, EA (2007) Weeds of California and Other Western States. Oakland, CA: University of California Department of Agriculture and Natural Resources Publication No. 3488. Volume 1. 848 pGoogle Scholar
Donaldson, RP, Luster, DG (1991) Multiple forms of plant cytochromes P-450. Plant Physiol 96:669–674CrossRefGoogle ScholarPubMed
Ellis, AT, Morgan, GD, Mueller, TC (2008) Mesosulfuron-resistant Italian ryegrass (Lolium multiflorum) biotype from Texas. Weed Technol 22:431–434CrossRefGoogle Scholar
Han, H, Yu, Q, Cawthray, GR, Powles, SB (2013) Enhanced herbicide metabolism induced by 2, 4-D in herbicide susceptible Lolium rigidum provides protection against diclofop-methyl. Pest Manag Sci 69:996–1000CrossRefGoogle ScholarPubMed
Hanson, BD, Wright, S, Sosnoskie, LM, Fischer, AJ, Jasieniuk, M, Roncoroni, JA, Hembree, KJ, Orloff, S, Shrestha, A, Al-Khatib, K (2014) Herbicide-resistant weeds challenge some signature cropping systems. Calif Agric 68:142–152CrossRefGoogle Scholar
Heap, I (2018) The International Survey of Herbicide Resistant Weeds. www.weedscience.org. Accessed: May 5, 2018Google Scholar
Hulting, AG, Dauer, JT, Hinds-Cook, B, Curtis, D, Koepke-Hill, RM, Mallory-Smith, C (2012) Management of Italian ryegrass (Lolium perenne ssp. multiflorum) in western Oregon with preemergence applications of pyroxasulfone in winter wheat. Weed Technol 26:230–235CrossRefGoogle Scholar
Jasieniuk, M, Ahmad, R, Sherwood, AM, Firestone, JL, Perez-Jones, A, Lanini, WT, Mallory-Smith, C, Stednick, Z (2008) Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: distribution, response to glyphosate, and molecular evidence for an altered target enzyme. Weed Sci 56:496–502CrossRefGoogle Scholar
Karn, E, Beffa, R, Jasieniuk, M (2018) Variation in response and resistance to glyphosate and glufosinate in California populations of Italian ryegrass (Lolium perenne ssp. multiflorum). Weed Sci 66: 168–179CrossRefGoogle Scholar
Kreuz, K, Fonné-Pfister, R (1992) Herbicide-insecticide interaction in maize: malathion inhibits cytochrome P450-dependent primisulfuron metabolism. Pestic Biochem Physiol 43:232–240CrossRefGoogle Scholar
Kuk, YI, Burgos, NR (2007) Cross-resistance profile of mesosulfuron-methyl-resistant Italian ryegrass in the southern United States. Pest Manag Sci 63:349–357CrossRefGoogle ScholarPubMed
Kuk, YI, Burgos, NR, Scott, RC (2008) Resistance profile of diclofop-resistant Italian ryegrass (Lolium multiflorum) to ACCase- and ALS-inhibiting herbicides in Arkansas, USA. Weed Sci 56:614–623CrossRefGoogle Scholar
Liu, M, Hulting, AG, Mallory-Smith, C (2014) Characterization of multiple-herbicide-resistant Italian ryegrass (Lolium perenne ssp. multiflorum). Pest Manag Sci 70:1145–1150CrossRefGoogle Scholar
Liu, M, Hulting, AG, Mallory-Smith, C (2016) Characterization of multiple herbicide-resistant Italian ryegrass (Lolium perenne ssp. multiflorum) populations from winter wheat fields in Oregon. Weed Sci 64:331–338CrossRefGoogle Scholar
Menegat, A, Bailly, GC, Aponte, R, Heinrich, GMT, Sievernich, B, Gerhards, R (2016) Acetohydroxyacid synthase (AHAS) amino acid substitution Asp376Glu in Lolium perenne: effect on herbicide efficacy and plant growth. J Plant Dis Prot 123:145–153CrossRefGoogle Scholar
Nandula, VK, Messersmith, CG (2000) Mechanism of wild oat (Avena fatua L.) resistance to imazamethabenz-methyl. Pestic Biochem Physiol 68:148–155CrossRefGoogle Scholar
Nandula, VK, Reddy, KN, Poston, DH, Rimando, AM, Duke, SO (2008) Glyphosate tolerance mechanism in Italian ryegrass (Lolium multiflorum) from Mississippi. Weed Sci 56:344–349CrossRefGoogle Scholar
Rauch, TA, Thill, DC, Gersdorf, SA, Price, WJ (2010) Widespread occurrence of herbicide-resistant ryegrass (Lolium multiflorum) in northern Idaho and eastern Washington. Weed Technol 24:281–288CrossRefGoogle Scholar
Ray, TB (1984) Site of action of chlorsulfuron. Plant Physiol 75:827–831CrossRefGoogle ScholarPubMed
Riar, DS, Norsworthy, JK, Srivastava, V, Nandula, V, Bond, JA, Scott, RC (2013) Physiological and molecular basis of acetolactate synthase-inhibiting herbicide resistance in barnyardgrass (Echinochloa crus-galli). Agric Food Chem 61: 278–289CrossRefGoogle Scholar
Saari, LL, Cotterman, JC, Smith, WF, Primiani, MM (1992) Sulfonylurea herbicide resistance in common chickweed, perennial ryegrass and Russian thistle. Pestic Biochem Physiol 42:110–118CrossRefGoogle Scholar
Simarmata, M, Kaufmann, JE, Penner, D (2003) Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum). Weed Sci 5:678–682CrossRefGoogle Scholar
Tan, MK, Preston, C, Wang, GX (2007) Molecular basis of multiple resistance to ACCase-inhibiting and ALS-inhibiting herbicides in Lolium rigidum. Weed Res 47:534–541CrossRefGoogle Scholar
Tehranchian, P, Nandula, V, Jugulam, M, Putta, K, Jasieniuk, M (2018) Multiple resistance to glyphosate, paraquat and ACCase-inhibiting herbicides in L. perenne ssp. multiflorum populations from California: confirmation and mechanisms of resistance. Pest Manag Sci 74:868–877CrossRefGoogle Scholar
Tranel, PJ, Wright, TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci 50:700–712CrossRefGoogle Scholar
Tranel, PJ, Wright, TR, Heap, IM (2018) Mutations in Herbicide-Resistant Weeds to ALS Inhibitors. www.weedscience.com. Accessed: July 31, 2018Google Scholar
Westerfield, WW (1945) A colorimetric determination of blood acetoin. J Biol Chem 161:495–502Google ScholarPubMed
Whitcomb, CE (1999) An introduction to ALS-inhibiting herbicides. Toxicol Ind Health 15:232–240.CrossRefGoogle ScholarPubMed
Wright, TR, Bascomb, NF, Sturner, SF, Penner, D (1998) Biochemical mechanism and molecular basis for ALS-inhibiting herbicide resistance in sugarbeet (Beta vulgaris) somatic cell selections. Weed Sci 46:13–23Google Scholar
Yu, Q, Han, H, Powles, SB (2008) Mutations of the ALS gene endowing resistance to ALS-inhibiting herbicides in Lolium rigidum populations. Pest Manag Sci 64:1229–1236CrossRefGoogle ScholarPubMed
Yu, Q, Powles, SB (2014) Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci 70:1340–1350CrossRefGoogle ScholarPubMed