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Resistance to glyphosate in Lolium rigidum. II. Uptake, translocation, and metabolism

Published online by Cambridge University Press:  12 June 2017

Paul C.C. Feng ,
James E. Pratley  and
Joseph A. Bohn
James E. Pratley
Affiliation:
Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
Joseph A. Bohn
Affiliation:
Monsanto Co., St. Louis, MO 63198
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Abstract

Experiments were conducted to determine potential mechanisms of glyphosate resistance in Lolium rigidum from Australia. 14C-Glyphosate uptake, translocation, and metabolism were compared between resistant (R) and sensitive (S) biotypes. The R seed (48118a) represented the F1 progenies of plants having survived a 1.73-kg ae ha−1 (4.8 L ha−1) application of a Roundup® formulation. The S seed was a sensitive biotype of L. rigidum from Australia. Plants (one to four tillers, 2 to 4 wk old) were presprayed with a high (1.26 kg ae ha−1) or a low (0.28 kg ae ha−1) dose of formulated glyphosate. The first leaf of the first tiller, which was shielded from the spray, was immediately treated with a 14C-glyphosate solution via manual application to the adaxial surface. Harvest was made 6 days after treatment (DAT), and glyphosate residues in the leaf wash, treated leaf, roots, and shoots were quantified based on radioactivity as percentage of applied dose. The overall radioactivity recoveries were very good (90.2 to 97.3% of applied dose). R and S plants showed comparable uptake at the high (79.2 vs. 78.0%) or the low (64.0 vs. 64.7%) doses of glyphosate. About one-half of the absorbed glyphosate in both R and S (32.9 to 38.3% appl.) was translocated into the plant and distributed almost equally into roots (13.6 to 16.0% appl.) and shoots (18.1 to 22.6% appl.). Autoradiography studies demonstrated no difference in tissue localization of glyphosate between the R and S plants. For metabolism studies, tissues from individual plants were homogenized in water, and extracts were analyzed by anion exchange high-pressure liquid chromatography (HPLC) with radioactivity detection. There was little to no metabolism of glyphosate in extracts from various tissues of either R or S plants. Based on these results, we conclude that neither uptake, translocation, nor metabolism play a major role in glyphosate resistance in L. rigidum.

Keywords

GlyphosateLolium rigidum, LOLRI, rigid ryegrassHerbicide resistanceglyphosate resistanceLOLRI
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 47 , Issue 4 , August 1999 , pp. 412 - 415
Copyright
Copyright © 1999 by the Weed Science Society of America 

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References

Literature Cited

Barry, G. E., Kishore, G. M., Padgette, S. R., et al. 1992. Inhibitors of amino acid biosynthesis: strategies for imparting glyphosate tolerance to crop plants. Pages 139–145 in Singh, B. K., Flores, H. E., and Shannon, J. C., eds. Biosynthesis and Molecular Regulation of Amino Acids in Plants. Rockville, MD: American Society of Plant Physiologists.Google Scholar
Bradshaw, L. D., Padgette, S. R., Kimball, S. K., and Wells, B. H. 1997. Perspectives on glyphosate resistance. Weed Technol. 11:189–198.CrossRefGoogle Scholar
Feng, P.C.C., Ryerse, J. S., and Sammons, R. D. 1998. Correlation of leaf damage with uptake and translocation of glyphosate in velvetleaf (Abutilon theophrasti). Weed Technol. 12:300–307.CrossRefGoogle Scholar
Franz, J. E., Mao, M. K., and Sikorski, J. A., 1997. Uptake, transport, and metabolism of glyphosate in plants. Pages 143–186 in Glyphosate: A Unique Global Herbicide. Washington, DC: American Chemical Society Monogr. 189.Google Scholar
Heap, I. M. 1997. The occurrence of herbicide-resistant weeds worldwide. Pestic. Sci. 51:235–243.3.0.CO;2-N>CrossRefGoogle Scholar
Holt, J. S., Powles, S. B., and Holtum, J.A.M. 1993. Mechanisms and agronomic aspects of herbicide resistance. Annu. Rev. Plant Physiol. Mol. Biol. 44:203–209.CrossRefGoogle Scholar
Johnston, D. T. and Faulkner, J. S. 1991. Herbicide resistance in the Graminaceae—a plant breeder's view. Pages 319–330 in Caseley, J. C., Cussans, G. W., and Atkin, R. T., eds. Herbicide Resistance in Weeds and Crops. Oxford: Butterworth-Heinemann.CrossRefGoogle Scholar
Klevorn, T. B. and Wyse, D. L. 1984. Effects of leaf girdling and rhizome girdling on glyphosate and photoassimilate distribution in quackgrass (Agropyron repens). Weed Sci. 32:402–407.CrossRefGoogle Scholar
Moss, S. R. and Rubin, B. 1993. Herbicide-resistant weeds: a worldwide perspective. J. Agric. Sci. 120:141–148.CrossRefGoogle Scholar
Powles, S. B., Holtum, J.A.M., Matthews, J. M., and Liljegren, D. R. 1990. Herbicide cross-resistance in rigid ryegrass (Lolium rigidum Gaud.). Pages 394–406 in Managing Resistance to Agrochemicals. Washington, DC: American Chemical Society Symposium Ser. 421.CrossRefGoogle Scholar
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci. 46:604–607.CrossRefGoogle Scholar
Pratley, J. E., Baines, P., Eberbach, R., Incerti, M., and Broster, J. 1996. Glyphosate resistance in annual ryegrass. Page 126 in Virgona, J. and Michalk, D., eds. Proceedings of the 11th Annual Conference of the Grasslands Society of New South Wales. Wagga Wagga, Australia: The Grasslands Society of NSW.Google Scholar
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Krueger, R. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci. 47:405–411.CrossRefGoogle Scholar
Preston, C., Tardif, F. J., Christopher, J. T., and Powles, S. B. 1996. Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic. Biochem. Physiol. 54:123–134.CrossRefGoogle Scholar
Tardif, F. J., Preston, C., and Powles, S. B. 1997. Mechanisms of herbicide multiple resistance in Lolium rigidum . Pages 117–124 in Prado, R. D., Jorrin, J., and Torres, L. G., eds. Weed and Crop Resistance to Herbicides. The Hague, The Netherlands: Kluwer.CrossRefGoogle Scholar
Wallace, R. W. and Bellinder, R. R. 1995. Glyphosate absorption and translocation in rust-infected quackgrass (Elytrigia repens). Weed Sci. 43:1–6.CrossRefGoogle Scholar
Westwood, J. H. and Weller, S. C. 1997. Cellular mechanisms influence differential glyphosate sensitivity in field bindweed (Convolvulus arvensis) biotypes. Weed Sci. 45:2–11.CrossRefGoogle Scholar
Westwood, J. H., Yerkes, C. N., and Weller, S. C. 1997. Absorption and translocation of glyphosate in tolerant and susceptible biotypes of field bindweed (Convolvulus arvensis). Weed Sci. 45:658–663.CrossRefGoogle Scholar