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Rigid Ryegrass (Lolium rigidum) Populations Containing a Target Site Mutation in EPSPS and Reduced Glyphosate Translocation Are More Resistant to Glyphosate

  • Yazid Bostamam (a1), Jenna M. Malone (a1), Fleur C. Dolman (a1), Peter Boutsalis (a1) and Christopher Preston (a1)...
Abstract

Glyphosate is widely used for weed control in the grape growing industry in southern Australia. The intensive use of glyphosate in this industry has resulted in the evolution of glyphosate resistance in rigid ryegrass. Two populations of rigid ryegrass from vineyards, SLR80 and SLR88, had 6- to 11-fold resistance to glyphosate in dose-response studies. These resistance levels were higher than two previously well-characterized glyphosate-resistant populations of rigid ryegrass (SLR77 and NLR70), containing a modified target site or reduced translocation, respectively. Populations SLR80 and SLR88 accumulated less glyphosate, 12 and 17% of absorbed glyphosate, in the shoot in the resistant populations compared with 26% in the susceptible population. In addition, a mutation within the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) where Pro106 had been substituted by either serine or threonine was identified. These two populations are more highly resistant to glyphosate as a consequence of expressing two different resistance mechanisms concurrently.

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Corresponding author's E-mail: jenna.malone@adelaide.edu.au
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L. Arnaud , F. Nurit , P. Ravanel , and M. Tissut 1994. Distribution of glyphosate and of its target enzyme inside wheat plants. Pestic. Sci. 40:217223.

S. R. Baerson , D. J. Rodriguez , M. Tran , Y. Feng , N. A. Biest , and G. M. Dill 2002. Glyphosate-resistant goosegrass: identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:12651275.

R. L. Chase and A. P. Appleby 1979. Effects of humidity and moisture stress on glyphosate control of Cyperus rotundus L. Weed Res. 19:241246.

H. Cruz-Hipolito , M. D. Osuna , A. Heredia , J. P. Ruiz-Santaella , and R. De Prado 2009. Nontarget mechanisms involved in glyphosate tolerance found in Canavalia ensiformis plants. J. Agric. Food Chem. 57:48444848.

T. A. Gaines , W. Zhang , D. Wang , B. Bukun , S. T. Chisholm , D. L. Shaner , S. J. Nissen , W. L. Patzoldt , P. J. Tranel , A. S. Culpepper , T. L. Grey , T. M. Webster , W. K. Vencill , R. D. Sammons , J. Jiang , C. Preston , J. E. Leach , and P. Westra 2010. Gene amplification confers glyphosate resistance in Amaranthus palmeri. Proc. Natl. Acad. Sci. USA. 107:10291034.

M. L. Healy-Fried , T. Funke , M. A. Priestman , H. Han , and E. Schonbrunn 2007. Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase. J. Biol. Chem. 282:3294932955.

M. Jasieniuk , R. Ahmad , A. M. Sherwood , J. L. Firestone , A. Perez-Jones , W. T. Lanini , C. Mallory-Smith , and Z. Stednick 2008. Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: distribution, response to glyphosate and molecular evidence for an altered target enzyme. Weed Sci. 56:496502.

S. S. Kaundun , I. A. Zelaya , R. P. Dale , A. J. Lycett , P. Carter , K. R. Sharples , and E. McIndoe 2008. Importance of the P106S target-site mutation in conferring resistance to glyphosate in a goosegrass (Eleusine indica) population from the Philippines. Weed Sci. 56:637646.

D. F. Lorraine-Colwill , S. B. Powles , T. R. Hawkes , and C. Preston 2001. Inheritance of evolved glyphosate resistance in Lolium rigidum (Gaud.). Theor. Appl. Genet. 102:545550.

V. K. Nandula , K. N. Reddy , D. H. Poston , A. M. Rimando , and S. O. Duke 2008. Glyphosate tolerance mechanism in Italian ryegrass (Lolium multiflorum) from Mississippi. Weed Sci. 56:344349.

C. H. Ng , R. Wickneswary , S. Salmijah , Y. T. Teng , and B. S. Ismail 2003. Gene polymorphisms in glyphosate-resistant and susceptible biotypes of Eleusine indica from Malaysia. Weed Res. 43:108115.

C. H. Ng , R. Wickneswary , S. Salmijah , Y. T. Teng , and B. S. Ismail 2004. Glyphosate resistance in Eleusine indica (L.) Gaertn. from different origins and polymerase chain reaction amplification of specific alleles. Aust. J. Agric. Res. 55:407414.

A. Perez-Jones , K. W. Park , N. Polge , J. Colquhoun , and C. Mallory-Smith 2007. Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta. 226:395404.

C. Preston 2010. Glyphosate-resistant rigid ryegrass in Australia. Pages 233247 in V. K. Nandula , ed. Glyphosate Resistance in Crops and Weeds: History, Development, and Management. Hoboken, NJ John Wiley & Sons.

C. Preston , A. M. Wakelin , F. C. Dolman , Y. Bostamam , and P. Boutsalis 2009. A decade of glyphosate-resistant Lolium around the world: mechanisms, genes, fitness and agronomic management. Weed Sci. 57:435441.

H. C. Steinrucken and N. Amrhein 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic-acid-3-phosphate synthase. Biochem. Biophys. Res. Commun. 94:12071212.

A. M. Wakelin , D. F. Lorraine-Colwill , and C. Preston 2004. Glyphosate resistance in four different populations of Lolium rigidum is associated zwith reduced translocation of glyphosate to meristematic zones. Weed Res. 44:453459.

A. M. Wakelin and C. Preston 2006. A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432440.

Q. Yu , A. Cairns , and S. B. Powles 2007. Glyphosate, paraquat and ACCase multiple herbicide resistance evolved in a Lolium rigidum biotype. Planta. 225:499513.

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Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
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