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Target Enzyme-Based Resistance to Clethodim in Lolium rigidum Populations in Australia

Published online by Cambridge University Press:  20 January 2017

Rupinder Kaur Saini ,
Jenna Malone ,
Christopher Preston  and
Gurjeet Gill
Rupinder Kaur Saini*
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Jenna Malone
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Christopher Preston
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Gurjeet Gill
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
*
Corresponding author's E-mail: rupinder.saini@adelaide.edu.au
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Abstract

Clethodim resistance was identified in 12 rigid ryegrass populations from winter cropping regions in four different states of Australia. Clethodim had failed to provide effective control of these populations in the field and resistance was suspected. Dose–response experiments confirmed resistance to clethodim and butroxydim in all populations. During 2012, the LD50 of resistant populations ranged from 10.2 to 89.3 g ha−1, making them 3 to 34–fold more resistant to clethodim than the susceptible population. Similarly, GR50 of resistant population varied from 8 to 37.1 g ha−1, which is 3 to 13.9–fold higher than the susceptible population. In 2013, clethodim-resistant populations were 7.8 to 35.3–fold more resistant to clethodim than the susceptible population. The higher resistance factor in 2013, especially in moderately resistant populations, could have been associated with lower ambient temperatures during the winter of 2013. These resistant populations had also evolved cross-resistance to butroxydim. The resistant populations required 1.3 to 6.6–fold higher butroxydim dose to achieve 50% mortality and 3 to 27–fold more butroxydim for 50% biomass reduction compared to the standard susceptible population. Sequencing of the target-site ACCase gene identified five known ACCase substitutions (isoleucine-1781-leucine, isoleucine-2041-asparagine, aspartate-2078-glycine, and cysteine-2088-arginine, and glycine-2096-alanine) in these populations. In nine populations, multiple ACCase mutations were present in different individuals. Furthermore, two alleles with different mutations were present in a single plant of rigid ryegrass in two populations.

Keywords

Clethodimrigid ryegrassLolium rigidum Gaudin.ACCase genebutroxydimclethodimresistance mechanismtarget-site mutation
Type
Weed Management
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 946 - 953
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Associate Editor for this paper: William Vencill, University of Georgia.

References

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