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Tuber development and propagation are inhibited by GA3 effects on the DELLA-dependent pathway in purple nutsedge (Cyperus rotundus)

Published online by Cambridge University Press:  04 September 2023

Chen-Yang Wei ,
Dan-Li Fan ,
Shu-Yu Liu ,
Shan-Chi Yi ,
Shi-Xian Yu ,
Guo-Chao Zhao Open the ORCID record for Guo-Chao Zhao [Opens in a new window] ,
Xiao-Liang Liu  and
Wen-Wei Tang Open the ORCID record for Wen-Wei Tang [Opens in a new window]
Chen-Yang Wei
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Dan-Li Fan
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Shu-Yu Liu
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Shan-Chi Yi
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Shi-Xian Yu
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Guo-Chao Zhao
Affiliation:
Undergraduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Xiao-Liang Liu
Affiliation:
Assistant Professor, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Wen-Wei Tang*
Affiliation:
Professor, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
*
Corresponding author: Wen-Wei Tang; Email: wenweitg@163.com
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Abstract

Purple nutsedge (Cyperus rotundus L.) is a globally distributed noxious weed that poses a significant challenge for control due to its fast and efficient propagation through the tuber, which is the primary reproductive organ. Gibberellic acid (GA3) has proven to be crucial for tuberization in tuberous plants. Therefore, understanding the relationship between GA3 and tuber development and propagation of C. rotundus will provide valuable information for controlling this weed. This study shows that the GA3 content decreases with tuber development, which corresponds to lower expression of bioactive GA3 synthesis genes (CrGA20ox, two CrGA3ox genes) and two upregulated GA3 catabolism genes (CrGA2ox genes), indicating that GA3 is involved in tuber development. Simultaneously, the expression of two CrDELLA genes and CrGID1 declines with tuber growth and decreased GA3, and yeast two-hybrid assays confirm that the GA3 signaling is DELLA-dependent. Furthermore, exogenous application of GA3 markedly reduces the number and the width of tubers and represses the growth of the tuber chain, further confirming the negative impact that GA3 has on tuber development and propagation. Taken together, these results demonstrate that GA3 is involved in tuber development and regulated by the DELLA-dependent pathway in C. rotundus and plays a negative role in tuber development and propagation.

Keywords

tuber chaintuberizationgibberellic acidDELLA protein
Type
Research Article
Information
Weed Science , Volume 71 , Issue 5 , September 2023 , pp. 453 - 461
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Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of the Weed Science Society of America

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Footnotes

Associate Editor: William Vencill, University of Georgia

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